Electron cryomicroscopy as a powerful tool in biomedical research

Miniaturizing EM Sample Preparation: Opportunities, Challenges, and “Visual Proteomics”

(2018) Stefan A. Arnold et al.   PROTEOMICS

Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures

(2017) Elizabeth H. Kellogg et al.   JOURNAL OF MOLECULAR BIOLOGY

Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4 Å

(2017) Beata Turoňová et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron cryo-microscopy structure of the mechanotransduction channel NOMPC

(2017) Peng Jin et al.   NATURE

Phase-plate cryo-EM structure of a class B GPCR–G-protein complex

(2017) Yi-Lynn Liang et al.   NATURE

Cryo-EM structure of a human spliceosome activated for step 2 of splicing

(2017) Karl Bertram et al.   NATURE

Cryo-EM structures of tau filaments from Alzheimer’s disease

(2017) Anthony W. P. Fitzpatrick et al.   NATURE

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Near-atomic structure of jasplakinolide-stabilized malaria parasite F-actin reveals the structural basis of filament instability

(2017) Sabrina Pospich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The molecular basis of Alzheimer's plaques

(2017) Sabrina Pospich et al.   SCIENCE

Fibril structure of amyloid-β(1–42) by cryo–electron microscopy

(2017) Lothar Gremer et al.   SCIENCE

Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

(2017) Maryam Khoshouei et al.   Nature Communications

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

(2017) Toshio Moriya et al.   Jove-Journal of Visualized Experiments

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection

(2016) Xin Gong et al.   CELL

Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating

(2016) Doreen Matthies et al.   CELL

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

CryoEM structure of yeast cytoplasmic exosome complex

(2016) Jun-Jie Liu et al.   CELL RESEARCH

Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study

(2016) Van-Mai Cao-Lormeau et al.   LANCET

Structure of the thermally stable Zika virus

(2016) Victor A. Kostyuchenko et al.   NATURE

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

Structure of spinach photosystem II–LHCII supercomplex at 3.2 Å resolution

(2016) Xuepeng Wei et al.   NATURE

Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body

(2016) L. J. Worrall et al.   NATURE

Cullin–RING ubiquitin E3 ligase regulation by the COP9 signalosome

(2016) Simone Cavadini et al.   NATURE

Structure of promoter-bound TFIID and model of human pre-initiation complex assembly

(2016) Robert K. Louder et al.   NATURE

Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution

(2016) Julian von der Ecken et al.   NATURE

A saposin-lipoprotein nanoparticle system for membrane proteins

(2016) Jens Frauenfeld et al.   NATURE METHODS

Membrane insertion of a Tc toxin in near-atomic detail

(2016) Christos Gatsogiannis et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the human 26S proteasome at a resolution of 3.9 Å

(2016) Andreas Schweitzer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation

(2016) Florian K. M. Schur et al.   SCIENCE

Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer

(2016) J. H. Lee et al.   SCIENCE

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

(2016) J. Kosinski et al.   SCIENCE

Visualizing the molecular sociology at the HeLa cell nuclear periphery

(2016) J. Mahamid et al.   SCIENCE

Structural and molecular basis for Ebola virus neutralization by protective human antibodies

(2016) J. Misasi et al.   SCIENCE

Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy

(2016) Ziao Fu et al.   STRUCTURE

Cryo-EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein

(2016) Monika Bokori-Brown et al.   Nature Communications

Membrane insertion of a Tc toxin in near-atomic detail

(2016) Christos Gatsogiannis et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy

(2015) Vincent Postis et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells

(2015) John C. Whitney et al.   CELL

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins

(2015) Rui Zhang et al.   CELL

Single-Particle Cryo-EM at Crystallographic Resolution

(2015) Yifan Cheng   CELL

Seeing tobacco mosaic virus through direct electron detectors

(2015) Simon A. Fromm et al.   JOURNAL OF STRUCTURAL BIOLOGY

A focused ion beam milling and lift-out approach for site-specific preparation of frozen-hydrated lamellas from multicellular organisms

(2015) Julia Mahamid et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the human 80S ribosome

(2015) Heena Khatter et al.   NATURE

Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase

(2015) Jianhua Zhao et al.   NATURE

Structure of the toxic core of α-synuclein from invisible crystals

(2015) Jose A. Rodriguez et al.   NATURE

Atomic structure of anthrax protective antigen pore elucidates toxin translocation

(2015) Jiansen Jiang et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

Correlated light and electron microscopy: ultrastructure lights up!

(2015) Pascal de Boer et al.   NATURE METHODS

Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization

(2015) L. Zhang et al.   SCIENCE

Structure of the voltage-gated calcium channel Cav1.1 complex

(2015) J. Wu et al.   SCIENCE

2.2 A resolution cryo-EM structure of  -galactosidase in complex with a cell-permeant inhibitor

(2015) A. Bartesaghi et al.   SCIENCE

The structure of the dynactin complex and its interaction with dynein

(2015) L. Urnavicius et al.   SCIENCE

Neurodegeneration and microtubule dynamics: death by a thousand cuts

(2015) Jyoti Dubey et al.   Frontiers in Cellular Neuroscience

Sample preparation of biological macromolecular assemblies for the determination of high-resolution structures by cryo-electron microscopy

(2015) Holger Stark et al.   Microscopy

High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis

(2014) Gregory M. Alushin et al.   CELL

Structure of a mammalian ryanodine receptor

(2014) Ran Zalk et al.   NATURE

Structure of a type IV secretion system

(2014) Harry H. Low et al.   NATURE

Mechanism of Tc toxin action revealed in molecular detail

(2014) Dominic Meusch et al.   NATURE

Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution

(2014) Zhen Yan et al.   NATURE

Structure of the F-actin–tropomyosin complex

(2014) Julian von der Ecken et al.   NATURE

Architecture and conformational switch mechanism of the ryanodine receptor

(2014) Rouslan G. Efremov et al.   NATURE

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrastable gold substrates for electron cryomicroscopy

(2014) C. J. Russo et al.   SCIENCE

Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy

(2014) G. McMullan et al.   ULTRAMICROSCOPY

Microtubules and Their Role in Cellular Stress in Cancer

(2014) Amelia L. Parker et al.   Frontiers in Oncology

A method to achieve homogeneous dispersion of large transmembrane complexes within the holes of carbon films for electron cryomicroscopy

(2013) Martin Cheung et al.   JOURNAL OF STRUCTURAL BIOLOGY

TRPV1 structures in distinct conformations reveal activation mechanisms

(2013) Erhu Cao et al.   NATURE

A syringe-like injection mechanism in Photorhabdus luminescens toxins

(2013) Christos Gatsogiannis et al.   NATURE

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Viral life cycles captured in three-dimensions with electron microscopy tomography

(2011) Chi-yu Fu et al.   Current Opinion in Virology

Negative staining and cryo-negative staining of macromolecules and viruses for TEM

(2010) Sacha De Carlo et al.   MICRON

The structural basis for membrane binding and pore formation by lymphocyte perforin

(2010) Ruby H. P. Law et al.   NATURE

Graphene Oxide: Structural Analysis and Application as a Highly Transparent Support for Electron Microscopy

(2009) Neil R. Wilson et al.   ACS Nano

Electron Crystallography as a Technique to Study the Structure on Membrane Proteins in a Lipidic Environment

(2009) Stefan Raunser et al.   Annual Review of Biophysics

Graphene oxide: A substrate for optimizing preparations of frozen-hydrated samples

(2009) Radosav S. Pantelic et al.   JOURNAL OF STRUCTURAL BIOLOGY

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

(2008) Xuekui Yu et al.   NATURE