Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

Production of HIV virus-like particles by transient transfection of CAP-T cells at bioreactor scale avoiding medium replacement

(2017) Sonia Gutiérrez-Granados et al.   JOURNAL OF BIOTECHNOLOGY

Accelerated mass production of influenza virus seed stocks in HEK-293 suspension cell cultures by reverse genetics

(2017) Ernest Milián et al.   VACCINE

Nanoescapology: progress toward understanding the endosomal escape of polymeric nanoparticles

(2017) Laura I. Selby et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

Viral vectors for gene therapy and gene modification approaches

(2016) Otto-Wilhelm Merten et al.   BIOCHEMICAL ENGINEERING JOURNAL

Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells

(2016) Laurence Delafosse et al.   JOURNAL OF BIOTECHNOLOGY

Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector

(2016) Joshua C Grieger et al.   MOLECULAR THERAPY

Gene therapy: Industrial strength

(2016) Eric Bender   NATURE

Cationic liposome–nucleic acid nanoparticle assemblies with applications in gene delivery and gene silencing

(2016) Ramsey N. Majzoub et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES

Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate

(2016) Alina Venereo-Sanchez et al.   VACCINE

Development and Optimization of AAV hFIX Particles by Transient Transfection in an iCELLis®Fixed-Bed Bioreactor

(2016) Alicia D. Powers et al.   Human Gene Therapy Methods

Manufacturing of recombinant adeno-associated viral vectors for clinical trials

(2016) Nathalie Clément et al.   Molecular Therapy-Methods & Clinical Development

Selection and optimization of transfection enhancer additives for increased virus-like particle production in HEK293 suspension cell cultures

(2015) Laura Cervera et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells

(2015) Sonia Gutiérrez-Granados et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Extended gene expression by medium exchange and repeated transient transfection for recombinant protein production enhancement

(2015) Laura Cervera et al.   BIOTECHNOLOGY AND BIOENGINEERING

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Editorial: Can modern vaccine technology pursue the success of traditional vaccine manufacturing?

(2015) Reingard Grabherr et al.   Biotechnology Journal

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors

(2015) Rachael S. Felberbaum   Biotechnology Journal

Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli

(2015) Elisa A. Ramírez et al.   BIOTECHNOLOGY LETTERS

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY PROGRESS

A simple plasmid-based transient gene expression method using High Five cells

(2015) Xiao Shen et al.   JOURNAL OF BIOTECHNOLOGY

Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale

(2015) W. Mark Abbott et al.   PROTEIN EXPRESSION AND PURIFICATION

Editorial: Can modern vaccine technology pursue the success of traditional vaccine manufacturing?

(2015) Reingard Grabherr et al.   Biotechnology Journal

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors

(2015) Rachael S. Felberbaum   Biotechnology Journal

Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene withBcl-xL

(2014) Matthew P. Zustiak et al.   Biotechnology Journal

Towards real-time monitoring of therapeutic protein quality in mammalian cell processes

(2014) Daniel A.M. Pais et al.   CURRENT OPINION IN BIOTECHNOLOGY

Better and faster: improvements and optimization for mammalian recombinant protein production

(2014) Steven C Almo et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

EB66 cell line, a duck embryonic stem cell-derived substrate for the industrial production of therapeutic monoclonal antibodies with enhanced ADCC activity

(2014) Stéphane Olivier et al.   mAbs

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Use of an anti-apoptotic CHO cell line for transient gene expression

(2013) Nichole F. Macaraeg et al.   BIOTECHNOLOGY PROGRESS

A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression

(2013) Katharine Cain et al.   BIOTECHNOLOGY PROGRESS

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

(2013) Volker Jäger et al.   BMC BIOTECHNOLOGY

Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring

(2013) Maria de los Milagros Bassani Molinas et al.   CYTOTECHNOLOGY

Recent advances in mammalian protein production

(2013) Ashok D. Bandaranayake et al.   FEBS LETTERS

Generation of HIV-1 Gag VLPs by transient transfection of HEK 293 suspension cell cultures using an optimized animal-derived component free medium

(2013) Laura Cervera et al.   JOURNAL OF BIOTECHNOLOGY

Virus-free transient protein production in Sf9 cells

(2013) Xiao Shen et al.   JOURNAL OF BIOTECHNOLOGY

A simple, robust and highly efficient transient expression system for producing antibodies

(2013) Tom Vink et al.   METHODS

Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

(2013) Ana Clara Mignaqui et al.   PLoS One

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

(2012) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Transient recombinant protein expression in a human amniocyte cell line: The CAP-T® cell system

(2012) Simon Fischer et al.   BIOTECHNOLOGY AND BIOENGINEERING

PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process

(2012) Qiuling Xie et al.   CYTOTECHNOLOGY

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

(2012) Zuzana Kadlecova et al.   MACROMOLECULAR BIOSCIENCE

The Possible “Proton Sponge ” Effect of Polyethylenimine (PEI) Does Not Include Change in Lysosomal pH

(2012) Rikke V Benjaminsen et al.   MOLECULAR THERAPY

Large-scale plasmid preparation for transient gene expression

(2011) Lu Cheng et al.   BIOTECHNOLOGY LETTERS

Scale-up and manufacturing of clinical-grade self-inactivating γ-retroviral vectors by transient transfection

(2011) J C M van der Loo et al.   GENE THERAPY

Transient gene expression in HEK293 and vero cells immobilised on microcarriers

(2011) Lukas Fliedl et al.   JOURNAL OF BIOTECHNOLOGY

A simple high-yielding process for transient gene expression in CHO cells

(2011) Yashas Rajendra et al.   JOURNAL OF BIOTECHNOLOGY

How to screen non-viral gene delivery systems in vitro?

(2011) Ethlinn V.B. van Gaal et al.   JOURNAL OF CONTROLLED RELEASE

Automation of large scale transient protein expression in mammalian cells

(2011) Yuguang Zhao et al.   JOURNAL OF STRUCTURAL BIOLOGY

A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

(2011) Céline Raymond et al.   METHODS

Design of Experiment in CHO and HEK transient transfection condition optimization

(2011) Frederic Bollin et al.   PROTEIN EXPRESSION AND PURIFICATION

Process optimization and scale-up for production of rabies vaccine live adenovirus vector (AdRG1.3)

(2011) Chun Fang Shen et al.   VACCINE

Mammalian cell transfection: the present and the future

(2010) Tae Kyung Kim et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting

(2010) Johannes Pichler et al.   BIOTECHNOLOGY AND BIOENGINEERING

Large-Scale Manufacture and Characterization of a Lentiviral Vector Produced for Clinical Ex Vivo Gene Therapy Application

(2010) Otto-Wilhelm Merten et al.   HUMAN GENE THERAPY

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

(2010) Seiichi Yamano et al.   MOLECULAR BIOTECHNOLOGY

Polycation cytotoxicity: a delicate matter for nucleic acid therapy—focus on polyethylenimine

(2010) Ladan Parhamifar et al.   Soft Matter

High-level protein expression in scalable CHO transient transfection

(2009) Jianxin Ye et al.   BIOTECHNOLOGY AND BIOENGINEERING

The heterogeneous nature of polyethylenimine-DNA complex formation affects transient gene expression

(2009) Xiangzong Han et al.   CYTOTECHNOLOGY

Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells

(2009) Nathan P. Gabrielson et al.   JOURNAL OF CONTROLLED RELEASE

Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes

(2009) You-Yeon Won et al.   JOURNAL OF CONTROLLED RELEASE

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

(2009) Sven Ansorge et al.   JOURNAL OF GENE MEDICINE

Fusion partners can increase the expression of recombinant interleukins via transient transfection in 2936E cells

(2009) Jane Carter et al.   PROTEIN SCIENCE

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

(2009) Sophie Nallet et al.   VACCINE

Glycan analysis in cell culture-based influenza vaccine production: Influence of host cell line and virus strain on the glycosylation pattern of viral hemagglutinin

(2009) Jana Schwarzer et al.   VACCINE

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

(2008) S. Wulhfard et al.   BIOTECHNOLOGY PROGRESS

Nonviral Vectors for Gene Delivery

(2008) Meredith A. Mintzer et al.   CHEMICAL REVIEWS

Development of a generic transient transfection process at 100 L scale

(2008) Ola Tuvesson et al.   CYTOTECHNOLOGY

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

(2008) Gaurav Backliwal et al.   NUCLEIC ACIDS RESEARCH

Reflections on more than 10 years of TGE approaches

(2008) Sabine Geisse   PROTEIN EXPRESSION AND PURIFICATION

Transient Gene Expression in Suspension HEK-293 Cells: Application to Large-Scale Protein Production

(2005) Lucia Baldi et al.   BIOTECHNOLOGY PROGRESS