Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

Developments in virus-like particle-based vaccines for HIV

(2013) Luigi Buonaguro et al.   Expert Review of Vaccines

Recent advances in mammalian protein production

(2013) Ashok D. Bandaranayake et al.   FEBS LETTERS

Generation of HIV-1 Gag VLPs by transient transfection of HEK 293 suspension cell cultures using an optimized animal-derived component free medium

(2013) Laura Cervera et al.   JOURNAL OF BIOTECHNOLOGY

Development and validation of a quantitation assay for fluorescently tagged HIV-1 virus-like particles

(2013) Sonia Gutiérrez-Granados et al.   JOURNAL OF VIROLOGICAL METHODS

A simple, robust and highly efficient transient expression system for producing antibodies

(2013) Tom Vink et al.   METHODS

CAP, a new human suspension cell line for influenza virus production

(2012) Yvonne Genzel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Transient recombinant protein expression in a human amniocyte cell line: The CAP-T® cell system

(2012) Simon Fischer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Virus-like particles as a highly efficient vaccine platform: Diversity of targets and production systems and advances in clinical development

(2012) Natasha Kushnir et al.   VACCINE

Cell line specific control of polyethylenimine-mediated transient transfection optimized with “Design of experiments” methodology

(2011) Ben C. Thompson et al.   BIOTECHNOLOGY PROGRESS

A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

(2011) Céline Raymond et al.   METHODS

HIV-Gag VLPs presenting trimeric HIV-1 gp140 spikes constitutively expressed in stable double transfected insect cell line

(2011) M. Tagliamonte et al.   VACCINE

Abrogation of contaminating RNA activity in HIV-1 Gag VLPs

(2011) Ziyaad Valley-Omar et al.   Virology Journal

Virus-like particles in vaccine development

(2010) António Roldão et al.   Expert Review of Vaccines

Reactive oxygen species, cellular redox systems, and apoptosis

(2010) Magdalena L. Circu et al.   FREE RADICAL BIOLOGY AND MEDICINE

Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system

(2010) M. Tagliamonte et al.   VACCINE

Fluorescence Fluctuation Spectroscopy on Viral-Like Particles Reveals Variable Gag Stoichiometry

(2009) Yan Chen et al.   BIOPHYSICAL JOURNAL

Optimization of chimeric HIV-1 virus-like particle production in a baculovirus-insect cell expression system

(2009) Sirika Pillay et al.   BIOTECHNOLOGY PROGRESS

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

(2009) Sven Ansorge et al.   JOURNAL OF GENE MEDICINE

High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts

(2009) Nunzia Scotti et al.   PLANTA

Development of a generic transient transfection process at 100 L scale

(2008) Ola Tuvesson et al.   CYTOTECHNOLOGY

Transient Transfection Factors for High-Level Recombinant Protein Production in Suspension Cultured Mammalian Cells

(2008) Chaoting Liu et al.   MOLECULAR BIOTECHNOLOGY

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

(2008) Gaurav Backliwal et al.   NUCLEIC ACIDS RESEARCH

Reflections on more than 10 years of TGE approaches

(2008) Sabine Geisse   PROTEIN EXPRESSION AND PURIFICATION

Influenza-pseudotyped Gag virus-like particle vaccines provide broad protection against highly pathogenic avian influenza challenge

(2008) Joel R. Haynes et al.   VACCINE