Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Intracellular Trafficking Pathways for Nuclear Delivery of Plasmid DNA Complexed with Highly Efficient Endosome Escape Polymers

(2014) Marianne Gillard et al.   BIOMACROMOLECULES

A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression

(2013) Katharine Cain et al.   BIOTECHNOLOGY PROGRESS

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

(2013) David L. Hacker et al.   PROTEIN EXPRESSION AND PURIFICATION

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

(2012) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

(2011) Yashas Rajendra et al.   BIOTECHNOLOGY LETTERS

Cell line specific control of polyethylenimine-mediated transient transfection optimized with “Design of experiments” methodology

(2011) Ben C. Thompson et al.   BIOTECHNOLOGY PROGRESS

A simple high-yielding process for transient gene expression in CHO cells

(2011) Yashas Rajendra et al.   JOURNAL OF BIOTECHNOLOGY

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level

(2009) Dominic J. Glover et al.   JOURNAL OF GENE MEDICINE

Balancing protection and release of DNA: tools to address a bottleneck of non-viral gene delivery

(2009) C. L. Grigsby et al.   Journal of the Royal Society Interface

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

(2008) S. Wulhfard et al.   BIOTECHNOLOGY PROGRESS

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

(2008) Gaurav Backliwal et al.   NUCLEIC ACIDS RESEARCH

Reflections on more than 10 years of TGE approaches

(2008) Sabine Geisse   PROTEIN EXPRESSION AND PURIFICATION