A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment
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Title
A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment
Authors
Keywords
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Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 112, Issue 5, Pages 977-986
Publisher
Wiley
Online
2014-12-12
DOI
10.1002/bit.25514
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Note: Only part of the references are listed.- Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene withBcl-xL
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- (2014) Aaron B. Bos et al. JOURNAL OF BIOTECHNOLOGY
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- (2013) Katharine Cain et al. BIOTECHNOLOGY PROGRESS
- A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression
- (2013) Olalekan Daramola et al. BIOTECHNOLOGY PROGRESS
- Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter
- (2013) Lianchun Fan et al. JOURNAL OF BIOTECHNOLOGY
- Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
- (2012) Yashas Rajendra et al. BIOTECHNOLOGY AND BIOENGINEERING
- Transient recombinant protein expression in a human amniocyte cell line: The CAP-T® cell system
- (2012) Simon Fischer et al. BIOTECHNOLOGY AND BIOENGINEERING
- Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells
- (2012) Amelie Croset et al. JOURNAL OF BIOTECHNOLOGY
- Mammalian cell protein expression for biopharmaceutical production
- (2011) Jianwei Zhu BIOTECHNOLOGY ADVANCES
- Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells
- (2011) Lianchun Fan et al. BIOTECHNOLOGY AND BIOENGINEERING
- A simple high-yielding process for transient gene expression in CHO cells
- (2011) Yashas Rajendra et al. JOURNAL OF BIOTECHNOLOGY
- A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications
- (2011) Céline Raymond et al. METHODS
- Design of Experiment in CHO and HEK transient transfection condition optimization
- (2011) Frederic Bollin et al. PROTEIN EXPRESSION AND PURIFICATION
- Biopharmaceutical benchmarks 2010
- (2010) Gary Walsh NATURE BIOTECHNOLOGY
- Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure
- (2010) Ka Fai Suen et al. PROTEIN EXPRESSION AND PURIFICATION
- Junk DNA enhances pEI-based non-viral gene delivery
- (2009) Ethlinn V.B. van Gaal et al. INTERNATIONAL JOURNAL OF PHARMACEUTICS
- Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
- (2008) Gaurav Backliwal et al. NUCLEIC ACIDS RESEARCH
- Reflections on more than 10 years of TGE approaches
- (2008) Sabine Geisse PROTEIN EXPRESSION AND PURIFICATION
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