Cysteine-reactive probes and their use in chemical proteomics

Activity-based probes for the multicatalytic proteasome

(2017) David S. Hewings et al.   FEBS Journal

Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics

(2017) Yiqing Zhou et al.   Molecular BioSystems

Isotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling

(2017) Masahiro Abo et al.   MOLECULAR PHARMACEUTICS

Global profiling of lysine reactivity and ligandability in the human proteome

(2017) Stephan M. Hacker et al.   Nature Chemistry

Chemoproteomics-Enabled Discovery of a Potent and Selective Inhibitor of the DNA Repair Protein MGMT

(2016) Chao Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optimization of Caged Electrophiles for Improved Monitoring of Cysteine Reactivity in Living Cells

(2016) Masahiro Abo et al.   CHEMBIOCHEM

Covalent Modulators of the Vacuolar ATPase

(2016) Ying-Chu Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Strained Cyclic Disulfides Enable Cellular Uptake by Reacting with the Transferrin Receptor

(2016) Daniel Abegg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Proteome-wide covalent ligand discovery in native biological systems

(2016) Keriann M. Backus et al.   NATURE

The MaxQuant computational platform for mass spectrometry-based shotgun proteomics

(2016) Stefka Tyanova et al.   Nature Protocols

A systematic study of protein labeling by fluorogenic probes using cysteine targeting vinyl sulfone-cyclooctyne tags

(2016) B. Söveges et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Chemical proteomic map of dimethyl fumarate–sensitive cysteines in primary human T cells

(2016) Megan M. Blewett et al.   Science Signaling

Divergent synthesis and identification of the cellular targets of deoxyelephantopins

(2016) Roman Lagoutte et al.   Nature Communications

Chemical proteomic map of dimethyl fumarate–sensitive cysteines in primary human T cells

(2016) Megan M. Blewett et al.   Science Signaling

Proteome-Wide Profiling of Targets of Cysteine reactive Small Molecules by Using Ethynyl Benziodoxolone Reagents

(2015) Daniel Abegg et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cellular uptake: lessons from supramolecular organic chemistry

(2015) Giulio Gasparini et al.   CHEMICAL COMMUNICATIONS

A Caged Electrophilic Probe for Global Analysis of Cysteine Reactivity in Living Cells

(2015) Masahiro Abo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Substrate-Competitive Activity-Based Profiling of Ester Prodrug Activating Enzymes

(2015) Hao Xu et al.   MOLECULAR PHARMACEUTICS

Organometallic palladium reagents for cysteine bioconjugation

(2015) Ekaterina V. Vinogradova et al.   NATURE

Fast and Highly Chemoselective Alkynylation of Thiols with Hypervalent Iodine Reagents Enabled through a Low Energy Barrier Concerted Mechanism

(2014) Reto Frei et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Design of Reversible, Cysteine-Targeted Michael Acceptors Guided by Kinetic and Computational Analysis

(2014) Shyam Krishnan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Investigating the Proteome Reactivity and Selectivity of Aryl Halides

(2014) D. Alexander Shannon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ

(2014) Jürgen Cox et al.   MOLECULAR & CELLULAR PROTEOMICS

Rapid, Stable, Chemoselective Labeling of Thiols with Julia-Kocieński-like Reagents: A Serum-Stable Alternative to Maleimide-Based Protein Conjugation

(2013) Narihiro Toda et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Covalent inhibition of SUMO and ubiquitin-specific cysteine proteases by an in situ thiol–alkyne addition

(2013) Stefanie Sommer et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Cleavable trifunctional biotin reagents for protein labelling, capture and release

(2013) Yinliang Yang et al.   CHEMICAL COMMUNICATIONS

Chemical Proteomics with Sulfonyl Fluoride Probes Reveals Selective Labeling of Functional Tyrosines in Glutathione Transferases

(2013) Christian Gu et al.   CHEMISTRY & BIOLOGY

1,3,5-Triazine as a Modular Scaffold for Covalent Inhibitors with Streamlined Target Identification

(2013) Ranjan Banerjee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases

(2013) Reggy Ekkebus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles

(2013) Chu Wang et al.   NATURE METHODS

Cysteine-reactive chemical probes based on a modular 4-aminopiperidine scaffold

(2013) Shalise M. Couvertier et al.   MedChemComm

Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present

(2012) Marcus Bantscheff et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Activity-Based Probes for Studying the Activity of Flavin-Dependent Oxidases and for the Protein Target Profiling of Monoamine Oxidase Inhibitors

(2012) Joanna M. Krysiak et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rugulactone and its Analogues Exert Antibacterial Effects through Multiple Mechanisms Including Inhibition of Thiamine Biosynthesis

(2012) Matthew B. Nodwell et al.   CHEMBIOCHEM

How Chemoproteomics Can Enable Drug Discovery and Development

(2012) Raymond E. Moellering et al.   CHEMISTRY & BIOLOGY

A Periodate-Cleavable Linker for Functional Proteomics under Slightly Acidic Conditions: Application for the Analysis of Intracellular Aspartic Proteases

(2012) Andreas Maurer et al.   JOURNAL OF PROTEOME RESEARCH

Confirming Target Engagement for Reversible Inhibitors in Vivo by Kinetically Tuned Activity-Based Probes

(2012) Alexander Adibekian et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Simple and Effective Cleavable Linker for Chemical Proteomics Applications

(2012) Yinliang Yang et al.   MOLECULAR & CELLULAR PROTEOMICS

Target profiling of 4-hydroxyderricin in S. aureus reveals seryl-tRNA synthetase binding and inhibition by covalent modification

(2012) Oliver A. Battenberg et al.   Molecular BioSystems

Methylsulfonyl Benzothiazole (MSBT): A Selective Protein Thiol Blocking Reagent

(2012) Dehui Zhang et al.   ORGANIC LETTERS

Antibiotic activity and target discovery of three-membered natural product-derived heterocycles in pathogenic bacteria

(2012) Maximilian Pitscheider et al.   Chemical Science

Vibralactone as a Tool to Study the Activity and Structure of the ClpP1P2 Complex from Listeria monocytogenes

(2011) Evelyn Zeiler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein palmitoylation and subcellular trafficking

(2011) Clara Aicart-Ramos et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Potent and Selective Inhibitors of GlutathioneS-Transferase Omega 1 That Impair Cancer Drug Resistance

(2011) Katsunori Tsuboi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Alkynes as an eco-compatible “on-call” functionality orthogonal to biological conditions in water

(2011) Nick Uhlig et al.   Chemical Science

Quantitation in Mass-Spectrometry-Based Proteomics

(2010) Waltraud X. Schulze et al.   Annual Review of Plant Biology

Comparative Analysis of Cleavable Azobenzene-Based Affinity Tags for Bioorthogonal Chemical Proteomics

(2010) Yu-Ying Yang et al.   CHEMISTRY & BIOLOGY

Cysteine Function Governs Its Conservation and Degeneration and Restricts Its Utilization on Protein Surfaces

(2010) Stefano M. Marino et al.   JOURNAL OF MOLECULAR BIOLOGY

Showdomycin as a Versatile Chemical Tool for the Detection of Pathogenesis-Associated Enzymes in Bacteria

(2010) Thomas Böttcher et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Quantitative reactivity profiling predicts functional cysteines in proteomes

(2010) Eranthie Weerapana et al.   NATURE

Palmitoylome profiling reveals S-palmitoylation–dependent antiviral activity of IFITM3

(2010) Jacob S Yount et al.   Nature Chemical Biology

Approaches to labeling and identification of active site residues in glycosidases

(2010) Stephen G. Withers et al.   PROTEIN SCIENCE

Molecular Mechanisms of 4-Hydroxy-2-nonenal and Acrolein Toxicity: Nucleophilic Targets and Adduct Formation

(2009) Richard M. LoPachin et al.   CHEMICAL RESEARCH IN TOXICOLOGY

A Suite of Activity-Based Probes for Human Cytochrome P450 Enzymes

(2009) Aaron T. Wright et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Activity-Based Proteome Profiling of Potential Cellular Targets of Orlistat − An FDA-Approved Drug with Anti-Tumor Activities

(2009) Peng-Yu Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enrichment and Site Mapping ofO-LinkedN-Acetylglucosamine by a Combination of Chemical/Enzymatic Tagging, Photochemical Cleavage, and Electron Transfer Dissociation Mass Spectrometry

(2009) Zihao Wang et al.   MOLECULAR & CELLULAR PROTEOMICS

Relative Quantification of Proteins in Human Cerebrospinal Fluids by MS/MS Using 6-Plex Isobaric Tags

(2008) Loïc Dayon et al.   ANALYTICAL CHEMISTRY

Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry

(2008) Benjamin F. Cravatt et al.   Annual Review of Biochemistry

Mitochondrial Protein Targets of Thiol-Reactive Electrophiles

(2008) Hansen L. Wong et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Identification of Protein Targets of 4-Hydroxynonenal Using Click Chemistry for ex Vivo Biotinylation of Azido and Alkynyl Derivatives

(2008) Andrew Vila et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Expanding the functional diversity of proteins through cysteine oxidation

(2008) Khalilah G Reddie et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

β-Lactams as Selective Chemical Probes for the in Vivo Labeling of Bacterial Enzymes Involved in Cell Wall Biosynthesis, Antibiotic Resistance, and Virulence

(2008) Isabell Staub et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Disparate proteome reactivity profiles of carbon electrophiles

(2008) Eranthie Weerapana et al.   Nature Chemical Biology

Iodoacetamide-induced artifact mimics ubiquitination in mass spectrometry

(2008) Michael L Nielsen et al.   NATURE METHODS

Deoxyelephantopin inhibits cancer cell proliferation and functions as a selective partial agonist against PPARγ

(2007) Gang Zou et al.   BIOCHEMICAL PHARMACOLOGY

Posterior Error Probabilities and False Discovery Rates: Two Sides of the Same Coin

(2007) Lukas Käll et al.   JOURNAL OF PROTEOME RESEARCH