Amino Acid-Selective Segmental Isotope Labeling of Multidomain Proteins for Structural Biology

Cell-free expression of disulfide-containing eukaryotic proteins for structural biology

(2012) Erich Michel et al.   FEBS Journal

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

(2012) Yuichi Minato et al.   JOURNAL OF BIOMOLECULAR NMR

High-yield Escherichia coli-based cell-free expression of human proteins

(2012) Erich Michel et al.   JOURNAL OF BIOMOLECULAR NMR

A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution

(2011) Jun Yokoyama et al.   ANALYTICAL BIOCHEMISTRY

Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O

(2011) Xun-Cheng Su et al.   JOURNAL OF BIOMOLECULAR NMR

Solution NMR spectroscopy of supra-molecular systems, why bother? A methyl-TROSY view

(2011) Lewis E. Kay   JOURNAL OF MAGNETIC RESONANCE

Protein trans-splicing and its use in structural biology: opportunities and limitations

(2010) Gerrit Volkmann et al.   Molecular BioSystems

Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR

(2010) T. L. Religa et al.   SCIENCE

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins

(2009) Lenka Skrisovska et al.   JOURNAL OF BIOMOLECULAR NMR

The GAIT system: a gatekeeper of inflammatory gene expression

(2009) Rupak Mukhopadhyay et al.   TRENDS IN BIOCHEMICAL SCIENCES

A simple method for amino acid selective isotope labeling of recombinant proteins in E. coli

(2008) Kit I. Tong et al.   JOURNAL OF BIOMOLECULAR NMR

A stress-responsive RNA switch regulates VEGFA expression

(2008) Partho Sarothi Ray et al.   NATURE

Transmembrane segment enhanced labeling as a tool for the backbone assignment of  -helical membrane proteins

(2008) S. Reckel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improved Segmental Isotope Labeling Methods for the NMR Study of Multidomain or Large Proteins: Application to the RRMs of Npl3p and hnRNP L

(2007) Lenka Skrisovska et al.   JOURNAL OF MOLECULAR BIOLOGY