Nanobodies: Chemical Functionalization Strategies and Intracellular Applications

N-terminal chemical protein labeling using the naturally split GOS-TerL intein

(2017) Anne-Lena Bachmann et al.   JOURNAL OF PEPTIDE SCIENCE

Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide

(2017) Misao Akishiba et al.   Nature Chemistry

Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells

(2017) Henry D. Herce et al.   Nature Chemistry

Nanobody-mediated resistance to Grapevine fanleaf virus in plants

(2017) Caroline Hemmer et al.   PLANT BIOTECHNOLOGY JOURNAL

Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling

(2017) Dominik Schumacher et al.   Chemical Science

Generation of Small Single Domain Nanobody Binders for Sensitive Detection of Testosterone by Electrochemical Impedance Spectroscopy

(2016) Guanghui Li et al.   ACS Applied Materials & Interfaces

Delivery of Non-Native Cargo into Mammalian Cells Using Anthrax Lethal Toxin

(2016) Amy E. Rabideau et al.   ACS Chemical Biology

From Anthramycin to Pyrrolobenzodiazepine (PBD)-Containing Antibody-Drug Conjugates (ADCs)

(2016) Julia Mantaj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer  -amyloid peptide

(2016) S. Rauth et al.   BIOCHEMICAL JOURNAL

Chemical Protein Modification through Cysteine

(2016) Smita B. Gunnoo et al.   CHEMBIOCHEM

Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities

(2016) Sam Massa et al.   Contrast Media & Molecular Imaging

Targeting the replisome with transduced monoclonal antibodies triggers lethal DNA replication stress in cancer cells

(2016) Dominique Desplancq et al.   EXPERIMENTAL CELL RESEARCH

Emerging site-specific bioconjugation strategies for radioimmunotracer development

(2016) Sam Massa et al.   Expert Opinion on Drug Delivery

Current Status: Site-Specific Antibody Drug Conjugates

(2016) Dominik Schumacher et al.   JOURNAL OF CLINICAL IMMUNOLOGY

Peptide-tags for site-specific protein labelling in vitro and in vivo

(2016) Jonathan Lotze et al.   Molecular BioSystems

Selective chemical labeling of proteins

(2016) Xi Chen et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Intracellular Delivery of Nanobodies for Imaging of Target Proteins in Live Cells

(2016) Ruth Röder et al.   PHARMACEUTICAL RESEARCH

Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery

(2016) Virginia J. Bruce et al.   PROTEIN SCIENCE

Nanobodies as Versatile Tools to Understand, Diagnose, Visualize and Treat Cancer

(2016) Isabel Van Audenhove et al.   EBioMedicine

Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time

(2016) Hsin-Yi Chiu et al.   Scientific Reports

Conformational Nanobodies Reveal Tethered Epidermal Growth Factor Receptor Involved in EGFR/ErbB2 Predimers

(2015) Damien Nevoltris et al.   ACS Nano

Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes

(2015) Peter C. Fridy et al.   ANALYTICAL BIOCHEMISTRY

Enzyme-Mediated Modification of Single-Domain Antibodies for Imaging Modalities with Different Characteristics

(2015) Mohammad Rashidian et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase

(2015) Dominik Schumacher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Locked by Design: A Conformationally Constrained Transglutaminase Tag Enables Efficient Site-Specific Conjugation

(2015) Vanessa Siegmund et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy

(2015) Andreas Plückthun   Annual Review of Pharmacology and Toxicology

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates

(2015) Atsushi Yamaguchi et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugates: The Nexus of Bioorthogonal Chemistry, Protein Engineering, and Drug Development

(2015) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

Construction of a biotinylated cameloid-like antibody for lable-free detection of apolipoprotein B-100

(2015) Henan Li et al.   BIOSENSORS & BIOELECTRONICS

Efficient Intracellular Delivery of Native Proteins

(2015) Diego S. D’Astolfo et al.   CELL

A Nanobody Activation Immunotherapeutic that Selectively Destroys HER2-Positive Breast Cancer Cells

(2015) Melissa A. Gray et al.   CHEMBIOCHEM

Microbial Transglutaminase and c-myc-Tag: A Strong Couple for the Functionalization of Antibody-Like Protein Scaffolds from Discovery Platforms

(2015) Patrick Dennler et al.   CHEMBIOCHEM

An emerging playbook for antibody–drug conjugates: lessons from the laboratory and clinic suggest a strategy for improving efficacy and safety

(2015) Penelope M Drake et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Camelid nanobodies: killing two birds with one stone

(2015) Aline Desmyter et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Visualizing posttranslational and epigenetic modifications of endogenous proteins in vivo

(2015) Hiroshi Kimura et al.   HISTOCHEMISTRY AND CELL BIOLOGY

HypE-specific Nanobodies as Tools to Modulate HypE-mediated Target AMPylation

(2015) Matthias C. Truttmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fully Human VHSingle Domains That Rival the Stability and Cleft Recognition of Camelid Antibodies

(2015) Romain Rouet et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nanobodies as Probes for Protein Dynamicsin Vitroand in Cells

(2015) Oleg Y. Dmitriev et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nanobodies and recombinant binders in cell biology

(2015) Jonas Helma et al.   JOURNAL OF CELL BIOLOGY

A Novel 111In-Labeled Anti-Prostate-Specific Membrane Antigen Nanobody for Targeted SPECT/CT Imaging of Prostate Cancer

(2015) K. L. S. Chatalic et al.   JOURNAL OF NUCLEAR MEDICINE

Specificin vivoknockdown of protein function by intrabodies

(2015) Andrea LJ Marschall et al.   mAbs

Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders

(2015) Evgenia Platonova et al.   METHODS

Monitoring Interactions and Dynamics of Endogenous Beta-catenin With Intracellular Nanobodies in Living Cells

(2015) Bjoern Traenkle et al.   MOLECULAR & CELLULAR PROTEOMICS

Hypoxia-Targeting Fluorescent Nanobodies for Optical Molecular Imaging of Pre-Invasive Breast Cancer

(2015) Aram S. A. van Brussel et al.   MOLECULAR IMAGING AND BIOLOGY

An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression inEscherichia colicombined with intein-mediated protein ligation

(2015) Duy Tien Ta et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode

(2015) Seongsu Kang et al.   Scientific Reports

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Use of 18F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer

(2015) Mohammad Rashidian et al.   ACS Central Science

Finding the Right (Bioorthogonal) Chemistry

(2014) David M. Patterson et al.   ACS Chemical Biology

A nanobody-based electrochemiluminescent immunosensor for sensitive detection of human procalcitonin

(2014) Henan Li et al.   ANALYST

Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability

(2014) Nicole Nischan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Labeling of Cysteine-Tagged Camelid Single-Domain Antibody-Fragments for Use in Molecular Imaging

(2014) Sam Massa et al.   BIOCONJUGATE CHEMISTRY

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

The Uptake of Arginine-Rich Cell-Penetrating Peptides: Putting the Puzzle Together

(2014) Roland Brock   BIOCONJUGATE CHEMISTRY

Promises and Pitfalls of Intracellular Delivery of Proteins

(2014) Ailing Fu et al.   BIOCONJUGATE CHEMISTRY

Development of nanobody-based flow injection chemiluminescence immunoassay for sensitive detection of human prealbumin

(2014) Lei Ma et al.   BIOSENSORS & BIOELECTRONICS

Delivery of Antibody Mimics into Mammalian Cells via Anthrax Toxin Protective Antigen

(2014) Xiaoli Liao et al.   CHEMBIOCHEM

More than add-on: chemoselective reactions for the synthesis of functional peptides and proteins

(2014) Dominik Schumacher et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1

(2014) Min Zhu et al.   Journal of Translational Medicine

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

Design of Synthetic Autonomous VH Domain Libraries and Structural Analysis of a VH Domain Bound to Vascular Endothelial Growth Factor

(2013) Xiaolei Ma et al.   JOURNAL OF MOLECULAR BIOLOGY

A Nanobody Binding to Non-Amyloidogenic Regions of the Protein Human Lysozyme Enhances Partial Unfolding but Inhibits Amyloid Fibril Formation

(2013) Erwin De Genst et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells

(2013) Guillaume Freund et al.   mAbs

Nanobody-Functionalized Polymersomes for Tumor-Vessel Targeting

(2013) Marjoke F. Debets et al.   MACROMOLECULAR BIOSCIENCE

A Highly Specific Gold Nanoprobe for Live-Cell Single-Molecule Imaging

(2013) Cécile Leduc et al.   NANO LETTERS

Site-specific N-terminal labeling of proteins using sortase-mediated reactions

(2013) Christopher S Theile et al.   Nature Protocols

Recombinant Probes for Visualizing Endogenous Synaptic Proteins in Living Neurons

(2013) Garrett G. Gross et al.   NEURON

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

(2013) A. B. Ward et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A conserved threonine spring-loads precursor for intein splicing

(2013) Albert K. Dearden et al.   PROTEIN SCIENCE

Visualization and targeted disruption of protein interactions in living cells

(2013) Henry D. Herce et al.   Nature Communications

Genetically encoded system to track histone modification in vivo

(2013) Yuko Sato et al.   Scientific Reports

Expression of Recombinant Antibodies

(2013) André Frenzel et al.   Frontiers in Immunology

Use of Fluorescent Probes: Their Effect on Cell Biology and Limitations

(2012) Ellen C. Jensen   Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology

Pharmacokinetic, Biodistribution, and Biophysical Profiles of TNF Nanobodies Conjugated to Linear or Branched Poly(ethylene glycol)

(2012) Yulia Vugmeyster et al.   BIOCONJUGATE CHEMISTRY

Nanobody-induced perturbation of LFA-1/L-plastin phosphorylation impairs MTOC docking, immune synapse formation and T cell activation

(2012) Sarah De Clercq et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Site-Specific Protein Modification Using Lipoic Acid Ligase and Bis-Aryl Hydrazone Formation

(2012) Justin D. Cohen et al.   CHEMBIOCHEM

Antibody Phage Display Libraries: Contributions to Oncology

(2012) Carmela Dantas-Barbosa et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Targeting breast carcinoma with radioiodinated anti-HER2 Nanobody

(2012) Marek Pruszynski et al.   NUCLEAR MEDICINE AND BIOLOGY

Quantitative Live Imaging of Endogenous DNA Replication in Mammalian Cells

(2012) Andrew Burgess et al.   PLoS One

Direct and Dynamic Detection of HIV-1 in Living Cells

(2012) Jonas Helma et al.   PLoS One

Preparation of unnatural N-to-N and C-to-C protein fusions

(2012) Martin D. Witte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Magnetosome Expression of Functional Camelid Antibody Fragments (Nanobodies) in Magnetospirillum gryphiswaldense

(2011) Anna Pollithy et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Two-in-One Antibody against HER3 and EGFR Has Superior Inhibitory Activity Compared with Monospecific Antibodies

(2011) Gabriele Schaefer et al.   CANCER CELL

Combined MUC1-specific nanobody-tagged PEG-polyethylenimine polyplex targeting and transcriptional targeting of tBid transgene for directed killing of MUC1 over-expressing tumour cells

(2011) Elham Sadeqzadeh et al.   JOURNAL OF CONTROLLED RELEASE

Teaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 Scaffold

(2011) Akiko Koide et al.   JOURNAL OF MOLECULAR BIOLOGY

Targeting antibodies to the cytoplasm

(2011) Andrea L.J. Marschall et al.   mAbs

Protein engineering: navigating between chance and reason

(2011) Monya Baker   NATURE METHODS

Fluorescent fusion protein knockout mediated by anti-GFP nanobody

(2011) Emmanuel Caussinus et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic  2-microglobulin variant

(2011) K. Domanska et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cortical Constriction During Abscission Involves Helices of ESCRT-III-Dependent Filaments

(2011) J. Guizetti et al.   SCIENCE

Potent Delivery of Functional Proteins into Mammalian Cellsin Vitroandin VivoUsing a Supercharged Protein

(2010) James J. Cronican et al.   ACS Chemical Biology

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications

(2010) J. Löfblom et al.   FEBS LETTERS

Structure and function of immunoglobulins

(2010) Harry W. Schroeder et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Fluorescent proteins at a glance

(2010) G.-J. Kremers et al.   JOURNAL OF CELL SCIENCE

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

(2010) Dmitriy M. Chudakov et al.   PHYSIOLOGICAL REVIEWS

Structural basis for high-affinity HER2 receptor binding by an engineered protein

(2010) C. Eigenbrot et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Effect of GFP tags on the localization of EB1 and EB1 fragments in vivo

(2009) Susan B. Skube et al.   Cytoskeleton

Yeast Display Evolution of a Kinetically Efficient 13-Amino Acid Substrate for Lipoic Acid Ligase

(2009) Sujiet Puthenveetil et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Origin and evolution of the adaptive immune system: genetic events and selective pressures

(2009) Martin F. Flajnik et al.   NATURE REVIEWS GENETICS

Selection of TNF-α binding affibody molecules using a β-lactamase protein fragment complementation assay

(2009) P.-Å. Löfdahl et al.   New Biotechnology

A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding

(2009) Carina Frauer et al.   NUCLEIC ACIDS RESEARCH

Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins

(2009) B. R. McNaughton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Functional diversity of ankyrin repeats in microbial proteins

(2009) Souhaila Al-Khodor et al.   TRENDS IN MICROBIOLOGY

Chemoselective Ligation and Modification Strategies for Peptides and Proteins

(2008) Christian P. R. Hackenberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Efficient Site-Specific Labeling of Proteins via Cysteines

(2008) Younggyu Kim et al.   BIOCONJUGATE CHEMISTRY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes

(2008) Laura Trinkle-Mulcahy et al.   JOURNAL OF CELL BIOLOGY

Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus

(2008) Anita Y. Lam et al.   JOURNAL OF STRUCTURAL BIOLOGY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

A Functional GFP Fusion for Imaging Clathrin-Mediated Endocytosis

(2008) Joshua Z. Rappoport et al.   TRAFFIC