Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities

A Versatile Approach for the Site-Specific Modification of Recombinant Antibodies Using a Combination of Enzyme-Mediated Bioconjugation and Click Chemistry

(2015) Karen Alt et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Antibody–Drug Conjugates: The Nexus of Bioorthogonal Chemistry, Protein Engineering, and Drug Development

(2015) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells

(2015) Guan-Yu Chen et al.   CHEMISTRY-A EUROPEAN JOURNAL

HypE-specific Nanobodies as Tools to Modulate HypE-mediated Target AMPylation

(2015) Matthias C. Truttmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phase I Study of 68Ga-HER2-Nanobody for PET/CT Assessment of HER2 Expression in Breast Carcinoma

(2015) M. Keyaerts et al.   JOURNAL OF NUCLEAR MEDICINE

Noninvasive imaging of immune responses

(2015) Mohammad Rashidian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use of 18F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer

(2015) Mohammad Rashidian et al.   ACS Central Science

Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency

(2015) Roger R. Beerli et al.   PLoS One

Enzyme-Mediated Site-Specific Bioconjugation of Metal Complexes to Proteins: Sortase-Mediated Coupling of Copper-64 to a Single-Chain Antibody

(2014) Brett M. Paterson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Labeling of Cysteine-Tagged Camelid Single-Domain Antibody-Fragments for Use in Molecular Imaging

(2014) Sam Massa et al.   BIOCONJUGATE CHEMISTRY

Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy

(2014) Markus Ritzefeld   CHEMISTRY-A EUROPEAN JOURNAL

Sortase-mediated ligations for the site-specific modification of proteins

(2014) Lena Schmohl et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Radiolabeled nanobodies as theranostic tools in targeted radionuclide therapy of cancer

(2014) Matthias D’Huyvetter et al.   Expert Opinion on Drug Delivery

Site-Specific Trastuzumab Maytansinoid Antibody–Drug Conjugates with Improved Therapeutic Activity through Linker and Antibody Engineering

(2014) Thomas H. Pillow et al.   JOURNAL OF MEDICINAL CHEMISTRY

Single-Chain Antibody Conjugated to a Cage Amine Chelator and Labeled with Positron-Emitting Copper-64 for Diagnostic Imaging of Activated Platelets

(2014) Karen Alt et al.   MOLECULAR PHARMACEUTICS

Targeted Radionuclide Therapy with A 177Lu-labeled Anti-HER2 Nanobody

(2014) Matthias D'Huyvetter et al.   Theranostics

Nanobody: The “Magic Bullet” for Molecular Imaging?

(2014) Rubel Chakravarty et al.   Theranostics

Rapid optical imaging of human breast tumour xenografts using anti-HER2 VHHs site-directly conjugated to IRDye 800CW for image-guided surgery

(2013) Marta Kijanka et al.   EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING

Camelid single-domain antibody-fragment engineering for (pre)clinicalin vivomolecular imaging applications: adjusting the bullet to its target

(2013) Jens De Vos et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Synthesis, Preclinical Validation, Dosimetry, and Toxicity of 68Ga-NOTA-Anti-HER2 Nanobodies for iPET Imaging of HER2 Receptor Expression in Cancer

(2013) C. Xavier et al.   JOURNAL OF NUCLEAR MEDICINE

Sortase-catalyzed in vitro functionalization of a HER2-specific recombinant Fab for tumor targeting of the plant cytotoxin gelonin

(2013) Petra Kornberger et al.   mAbs

Bio-Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications

(2012) Melissa K. M. Leung et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Design of Ca2+-independentStaphylococcus aureussortase A mutants

(2012) Hidehiko Hirakawa et al.   BIOTECHNOLOGY AND BIOENGINEERING

Novel Single-Chain Antibody-Targeted Microbubbles for Molecular Ultrasound Imaging of Thrombosis

(2012) Xiaowei Wang et al.   CIRCULATION

Development of177Lu-nanobodies for radioimmunotherapy of HER2-positive breast cancer: evaluation of different bifunctional chelators

(2012) Matthias D'Huyvetter et al.   Contrast Media & Molecular Imaging

Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates

(2012) Ben-Quan Shen et al.   NATURE BIOTECHNOLOGY

Preparation of unnatural N-to-N and C-to-C protein fusions

(2012) Martin D. Witte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Making and Breaking Peptide Bonds: Protein Engineering Using Sortase

(2011) Maximilian Wei-Lin Popp et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering of an anti-epidermal growth factor receptor antibody to single chain format and labeling by sortase A-mediated protein ligation

(2011) Mariusz P. Madej et al.   BIOTECHNOLOGY AND BIOENGINEERING

The two faces of His-tag: Immune response versus ease of protein purification

(2011) Theodore W. Randolph   Biotechnology Journal

Enzymatic Single-Chain Antibody Tagging

(2011) H.T. Ta et al.   CIRCULATION RESEARCH

Preclinical screening of anti-HER2 nanobodies for molecular imaging of breast cancer

(2011) Ilse Vaneycken et al.   FASEB JOURNAL

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure ofStreptococcus pyogenesSortase A

(2009) Paul R. Race et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY