Specificin vivoknockdown of protein function by intrabodies

Viral suppression function of intracellular antibody against C-terminal domain of rabies virus phosphoprotein

(2015) Yang Liu et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

The Translocon Protein Sec61 Mediates Antigen Transport from Endosomes in the Cytosol for Cross-Presentation to CD8+ T Cells

(2015) Matthias Zehner et al.   IMMUNITY

Intracellular Acidosis Promotes Mitochondrial Apoptosis Pathway: Role of EMMPRIN Down-regulation via Specific Single-chain Fv Intrabody

(2015) Patcharin Thammasit et al.   Journal of Cancer

Early Endosomal Escape of a Cyclic Cell-Penetrating Peptide Allows Effective Cytosolic Cargo Delivery

(2014) Ziqing Qian et al.   BIOCHEMISTRY

Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells

(2014) Philipp D. Kaiser et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Mutational approaches to improve the biophysical properties of human single-domain antibodies

(2014) Dae Young Kim et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2

(2014) S Sangboonruang et al.   CANCER GENE THERAPY

Toxicity of targeted therapy: Implications for response and impact of genetic polymorphisms

(2014) Sariah Liu et al.   CANCER TREATMENT REVIEWS

Small-Molecule Inhibitors of the Receptor Tyrosine Kinases: Promising Tools for Targeted Cancer Therapies

(2014) Mohammad Hojjat-Farsangi   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Functional inhibition of β-catenin-mediatedWnt signaling by intracellular VHHantibodies

(2014) Laura E Newnham et al.   mAbs

Delivery of antibodies to the cytosol

(2014) Andrea LJ Marschall et al.   mAbs

Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies

(2014) Andrea LJ Marschall et al.   mAbs

Directed Evolution of Human Heavy Chain Variable Domain (VH) Using In Vivo Protein Fitness Filter

(2014) Dong-Sik Kim et al.   PLoS One

In-Cell Intrabody Selection from a Diverse Human Library Identifies C12orf4 Protein as a New Player in Rodent Mast Cell Degranulation

(2014) Elsa Mazuc et al.   PLoS One

Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

(2014) Dujduan Waraho-Zhmayev et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Conformational targeting of intracellular Aβ oligomers demonstrates their pathological oligomerization inside the endoplasmic reticulum

(2014) Giovanni Meli et al.   Nature Communications

Current Progress in Gene Delivery Technology Based on Chemical Methods and Nano-carriers

(2014) Lian Jin et al.   Theranostics

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Insertional Mutagenesis by Retroviral Vectors: Current Concepts and Methods of Analysis

(2013) Sean Knight et al.   CURRENT GENE THERAPY

The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

(2013) Anne-Sophie Rinaldi et al.   EXPERIMENTAL CELL RESEARCH

History of gene therapy

(2013) Thomas Wirth et al.   GENE

RNA-based mechanisms regulating host-virus interactions

(2013) Rui Zhou et al.   IMMUNOLOGICAL REVIEWS

A bispecific transmembrane antibody simultaneously targeting intra- and extracellular epitopes of the epidermal growth factor receptor inhibits receptor activation and tumor cell growth

(2013) Nina Müller et al.   INTERNATIONAL JOURNAL OF CANCER

In vivoantitumor effect of an intracellular single-chain antibody fragment against the E7 oncoprotein of human papillomavirus 16

(2013) Luisa Accardi et al.   INTERNATIONAL JOURNAL OF CANCER

Antibody light chain variable domains and their biophysically improved versions for human immunotherapy

(2013) Dae Young Kim et al.   mAbs

Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells

(2013) Guillaume Freund et al.   mAbs

Targeting of ADAMTS5's ancillary domain with the recombinant mAb CRB0017 ameliorates disease progression in a spontaneous murine model of osteoarthritis

(2013) R. Chiusaroli et al.   OSTEOARTHRITIS AND CARTILAGE

Specific In Vivo Labeling of Tyrosinated α-Tubulin and Measurement of Microtubule Dynamics Using a GFP Tagged, Cytoplasmically Expressed Recombinant Antibody

(2013) Lynne Cassimeris et al.   PLoS One

A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions

(2013) Ole Aalund Mandrup et al.   PLoS One

Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment

(2012) Francesca Paoletti et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Fc-fusion proteins: new developments and future perspectives

(2012) Daniel M. Czajkowsky et al.   EMBO Molecular Medicine

Antibodies for all: The case for genome-wide affinity reagents

(2012) Sachdev S. Sidhu   FEBS LETTERS

Understanding lentiviral vector chromatin targeting: working to reduce insertional mutagenic potential for gene therapy

(2012) C Papayannakos et al.   GENE THERAPY

Fusion to a highly charged proteasomal retargeting sequence increases soluble cytoplasmic expression and efficacy of diverse anti-synuclein intrabodies

(2012) Shubhada N. Joshi et al.   mAbs

Dual targeting strategies with bispecific antibodies

(2012) Roland Kontermann   mAbs

Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells

(2012) Congcong Zhang et al.   PLoS One

The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated

(2012) Sreejith Raran-Kurussi et al.   PLoS One

Potent and Selective Phosphopeptide Mimetic Prodrugs Targeted to the Src Homology 2 (SH2) Domain of Signal Transducer and Activator of Transcription 3

(2011) Pijus K. Mandal et al.   JOURNAL OF MEDICINAL CHEMISTRY

Engineering Antibody Fitness and Function Using Membrane-Anchored Display of Correctly Folded Proteins

(2011) Amy J. Karlsson et al.   JOURNAL OF MOLECULAR BIOLOGY

Targeting antibodies to the cytoplasm

(2011) Andrea L.J. Marschall et al.   mAbs

Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum

(2011) Thomas Böldicke et al.   METHODS

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

A roadmap to generate renewable protein binders to the human proteome

(2011) Karen Colwill et al.   NATURE METHODS

State-of-the-art gene-based therapies: the road ahead

(2011) Mark A. Kay   NATURE REVIEWS GENETICS

Bifunctional Anti-Huntingtin Proteasome-Directed Intrabodies Mediate Efficient Degradation of Mutant Huntingtin Exon 1 Protein Fragments

(2011) David C. Butler et al.   PLoS One

Defining the Role of Essential Genes in Human Disease

(2011) Jonathan E. Dickerson et al.   PLoS One

Selection for intrabody solubility in mammalian cells using GFP fusions

(2011) Laurence Guglielmi et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Targeting the hepatitis B virus precore antigen with a novel IgNAR single variable domain intrabody

(2011) Renae Walsh et al.   VIROLOGY

Treatment with a Toll-like receptor inhibitory GpG oligonucleotide delays and attenuates lupus nephritis in NZB/W mice

(2010) Kareem L. Graham et al.   AUTOIMMUNITY

Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation

(2010) Carsten J Kirschning et al.   BMC BIOTECHNOLOGY

A human scFv antibody generation pipeline for proteome research

(2010) Michael Hust et al.   JOURNAL OF BIOTECHNOLOGY

A Single-Domain Llama Antibody Potently Inhibits the Enzymatic Activity of Botulinum Neurotoxin by Binding to the Non-Catalytic α-Exosite Binding Region

(2010) Jianbo Dong et al.   JOURNAL OF MOLECULAR BIOLOGY

Constitutive and conditional RNAi transgenesis in mice

(2010) Aljoscha Kleinhammer et al.   METHODS

Minimum information about a protein affinity reagent (MIAPAR)

(2010) Julie Bourbeillon et al.   NATURE BIOTECHNOLOGY

Dissecting the involvement of tropomyosin-related kinase A and p75 neurotrophin receptor signaling in NGF deficit-induced neurodegeneration

(2010) S. Capsoni et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Camelid single domain antibodies (VHHs) as neuronal cell intrabody binding agents and inhibitors of Clostridium botulinum neurotoxin (BoNT) proteases

(2010) Jacqueline M. Tremblay et al.   TOXICON

Generating recombinant antibodies to the complete human proteome

(2010) Stefan Dübel et al.   TRENDS IN BIOTECHNOLOGY

Enhanced internalization and endosomal escape of dual-functionalized poly(ethyleneimine)s polyplex with diphtheria toxin T and R domains

(2009) Shinji Kakimoto et al.   BIOMEDICINE & PHARMACOTHERAPY

Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9

(2009) Nutjeera Intasai et al.   IMMUNOBIOLOGY

Intracellular Expression of a Single Domain Antibody Reduces Cytotoxicity of 15-Acetyldeoxynivalenol in Yeast

(2009) Patrick J. Doyle et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selection and affinity maturation of human antibodies against rabies virus from a scFv gene library using ribosome display

(2009) Xiao-Ling Zhao et al.   JOURNAL OF BIOTECHNOLOGY

Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies

(2009) B. Serruys et al.   JOURNAL OF GENERAL VIROLOGY

Direct in Vivo Intracellular Selection of Conformation-sensitive Antibody Domains Targeting Alzheimer's Amyloid-β Oligomers

(2009) Giovanni Meli et al.   JOURNAL OF MOLECULAR BIOLOGY

Generating Differentially Targeted Amyloid-β Specific Intrabodies as a Passive Vaccination Strategy for Alzheimer's Disease

(2009) Kelly L Sudol et al.   MOLECULAR THERAPY

Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

(2009) Aimee L. Jackson et al.   NATURE REVIEWS DRUG DISCOVERY

Towards proteome scale antibody selections using phage display

(2009) Michael Mersmann et al.   New Biotechnology

Conformational Targeting of Fibrillar Polyglutamine Proteins in Live Cells Escalates Aggregation and Cytotoxicity

(2009) Erik Kvam et al.   PLoS One

Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism

(2009) D. Waraho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation and Functional Characterization of Single Domain Antibody Modulators of Caspase-3 and Apoptosis

(2008) Katrina McGonigal et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Cloning of apoB intrabodies: Specific knockdown of apoB in HepG2 cells

(2008) Wei Liao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

(2008) Khajornsak Tragoolpua et al.   BMC BIOTECHNOLOGY

Delivery Vehicles for Small Interfering RNA In Vivo

(2008) Antonin R. de Fougerolles   HUMAN GENE THERAPY

In vivo selection of intrabodies specifically targeting protein–protein interactions: A general platform for an “undruggable” class of disease targets☆

(2008) Michela Visintin et al.   JOURNAL OF BIOTECHNOLOGY

Functional knockdown of VCAM-1 at the posttranslational level with ER retained antibodies

(2008) N. Strebe et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The Human Combinatorial Antibody Library HuCAL GOLD Combines Diversification of All Six CDRs According to the Natural Immune System with a Novel Display Method for Efficient Selection of High-Affinity Antibodies

(2008) Christine Rothe et al.   JOURNAL OF MOLECULAR BIOLOGY

Efficient Isolation of Soluble Intracellular Single-chain Antibodies using the Twin-arginine Translocation Machinery

(2008) Adam C. Fisher et al.   JOURNAL OF MOLECULAR BIOLOGY

Isolation of human monoclonal antibodies by mammalian cell display

(2008) R. R. Beerli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA