Current Status: Site-Specific Antibody Drug Conjugates

Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase

(2015) Dominik Schumacher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Locked by Design: A Conformationally Constrained Transglutaminase Tag Enables Efficient Site-Specific Conjugation

(2015) Vanessa Siegmund et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Exocyclic Olefinic Maleimides: Synthesis and Application for Stable and Thiol-Selective Bioconjugation

(2015) Dimpy Kalia et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Current methods for the synthesis of homogeneous antibody–drug conjugates

(2015) Alicja M. Sochaj et al.   BIOTECHNOLOGY ADVANCES

Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

(2015) Patrick G. Holder et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Novel antibody–antibiotic conjugate eliminates intracellular S. aureus

(2015) Sophie M. Lehar et al.   NATURE

Reducing hydrophobicity of homogeneous antibody-drug conjugates improves pharmacokinetics and therapeutic index

(2015) Robert P Lyon et al.   NATURE BIOTECHNOLOGY

A plug-and-play approach to antibody-based therapeutics via a chemoselective dual click strategy

(2015) Antoine Maruani et al.   Nature Communications

Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency

(2015) Roger R. Beerli et al.   PLoS One

Antibody-Drug Conjugates: An Emerging Concept in Cancer Therapy

(2014) Ravi V. J. Chari et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bridging Disulfides for Stable and Defined Antibody Drug Conjugates

(2014) George Badescu et al.   BIOCONJUGATE CHEMISTRY

Auristatin Antibody Drug Conjugate Physical Instability and the Role of Drug Payload

(2014) Yilma T. Adem et al.   BIOCONJUGATE CHEMISTRY

Statistical Modeling of the Drug Load Distribution on Trastuzumab Emtansine (Kadcyla), a Lysine-Linked Antibody Drug Conjugate

(2014) Michael T. Kim et al.   BIOCONJUGATE CHEMISTRY

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugation through Glycoengineering

(2014) Qun Zhou et al.   BIOCONJUGATE CHEMISTRY

Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes

(2014) Penelope M. Drake et al.   BIOCONJUGATE CHEMISTRY

The tubulin code: Molecular components, readout mechanisms, and functions

(2014) Carsten Janke   JOURNAL OF CELL BIOLOGY

Mechanism-Based Pharmacokinetic/Pharmacodynamic Model for THIOMAB™ Drug Conjugates

(2014) Siddharth Sukumaran et al.   PHARMACEUTICAL RESEARCH

A general approach to site-specific antibody drug conjugates

(2014) Feng Tian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzyme-Mediated Methodology for the Site-Specific Radiolabeling of Antibodies Based on Catalyst-Free Click Chemistry

(2013) Brian M. Zeglis et al.   BIOCONJUGATE CHEMISTRY

Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates

(2013) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

Mass Spectrometric Characterization of Transglutaminase Based Site-Specific Antibody–Drug Conjugates

(2013) Santiago E. Farias et al.   BIOCONJUGATE CHEMISTRY

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

Structural basis of tubulin tyrosination by tubulin tyrosine ligase

(2013) Andrea E. Prota et al.   JOURNAL OF CELL BIOLOGY

Efficient N-Terminal Labeling of Proteins by Use of Sortase

(2012) Daniel J. Williamson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Traceless Coupling of Potent Cytotoxic Drugs to Recombinant Antibodies for Pharmacodelivery

(2012) Giulio Casi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates

(2012) Ben-Quan Shen et al.   NATURE BIOTECHNOLOGY

A Pictet-Spengler ligation for protein chemical modification

(2012) P. Agarwal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro Sortagging of an Antibody Fab Fragment: Overcoming Unproductive Reactions of Sortase with Water and Lysine Side Chains

(2011) Sina Möhlmann et al.   CHEMBIOCHEM

Antibody-Drug Conjugates of Calicheamicin Derivative: Gemtuzumab Ozogamicin and Inotuzumab Ozogamicin

(2011) A. D. Ricart   CLINICAL CANCER RESEARCH

Bypassing glycosylation: engineering aglycosylated full-length IgG antibodies for human therapy

(2011) Sang Taek Jung et al.   CURRENT OPINION IN BIOTECHNOLOGY

Synthesis and Evaluation of Hydrophilic Linkers for Antibody–Maytansinoid Conjugates

(2011) Robert Y. Zhao et al.   JOURNAL OF MEDICINAL CHEMISTRY

Polymeric Dibromomaleimides As Extremely Efficient Disulfide Bridging Bioconjugation and Pegylation Agents

(2011) Mathew W. Jones et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase

(2010) Simone Jeger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins

(2010) Darius Ghaderi et al.   NATURE BIOTECHNOLOGY

Site Specific Conjugation of Fluoroprobes to the Remodeled Fc N-Glycans of Monoclonal Antibodies Using Mutant Glycosyltransferases: Application for Cell Surface Antigen Detection

(2009) Elizabeth Boeggeman et al.   BIOCONJUGATE CHEMISTRY

Metabolic glycoengineering: Sialic acid and beyond

(2009) Jian Du et al.   GLYCOBIOLOGY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY