An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression inEscherichia colicombined with intein-mediated protein ligation

Nanobody-based cancer therapy of solid tumors

(2015) Marta Kijanka et al.   Nanomedicine

Cloning, expression and purification of the human Islet Amyloid Polypeptide (hIAPP) from Escherichia coli

(2015) Diana C. Rodriguez Camargo et al.   PROTEIN EXPRESSION AND PURIFICATION

Cell-free identification of novel N-myristoylated proteins from complementary DNA resources using bioorthogonal myristic acid analogues

(2014) Emi Takamitsu et al.   ANALYTICAL BIOCHEMISTRY

New Generation of Bioorthogonally Applicable Fluorogenic Dyes with Visible Excitations and Large Stokes Shifts

(2014) András Herner et al.   BIOCONJUGATE CHEMISTRY

Regio- and Chemoselective Immobilization of Proteins on Gold Surfaces

(2014) Seoung-ryoung Choi et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Labeling of Cysteine-Tagged Camelid Single-Domain Antibody-Fragments for Use in Molecular Imaging

(2014) Sam Massa et al.   BIOCONJUGATE CHEMISTRY

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Orientation of llama antibodies strongly increases sensitivity of biosensors

(2014) Anke K. Trilling et al.   BIOSENSORS & BIOELECTRONICS

Recombinant expression of antimicrobial peptides using a novel self-cleaving aggregation tag in Escherichia coli

(2014) Chao Luan et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Intein Applications: From Protein Purification and Labeling to Metabolic Control Methods

(2014) David W. Wood et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Advances in fluorescence labeling strategies for dynamic cellular imaging

(2014) Kevin M Dean et al.   Nature Chemical Biology

Site-Specific Bioconjugation of a Murine Dihydrofolate Reductase Enzyme by Copper(I)-Catalyzed Azide-Alkyne Cycloaddition with Retained Activity

(2014) Sung In Lim et al.   PLoS One

An optimized intein-mediated protein ligation approach for the efficient cyclization of cysteine-rich proteins

(2014) Katsiaryna Tarasava et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Nanobody: The “Magic Bullet” for Molecular Imaging?

(2014) Rubel Chakravarty et al.   Theranostics

SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS AND THEIR APPLICATIONS TO THERAPEUTIC ANTIBODIES

(2014) Remko van Vught et al.   Computational and Structural Biotechnology Journal

Site-Specific Epitope Tagging of G Protein-Coupled Receptors by Bioorthogonal Modification of a Genetically Encoded Unnatural Amino Acid

(2013) Saranga Naganathan et al.   BIOCHEMISTRY

Protein Engineering For Directed Immobilization

(2013) Erik Steen Redeker et al.   BIOCONJUGATE CHEMISTRY

Bioorthogonal, two-component delivery systems based on antibody and drug-loaded nanocarriers for enhanced internalization of nanotherapeutics

(2013) Sudath Hapuarachchige et al.   BIOMATERIALS

Improving bioorthogonal protein ubiquitylation by click reaction

(2013) Daniel Schneider et al.   BIOORGANIC & MEDICINAL CHEMISTRY

A Pretargeted PET Imaging Strategy Based on Bioorthogonal Diels-Alder Click Chemistry

(2013) B. M. Zeglis et al.   JOURNAL OF NUCLEAR MEDICINE

Nanobody-Functionalized Polymersomes for Tumor-Vessel Targeting

(2013) Marjoke F. Debets et al.   MACROMOLECULAR BIOSCIENCE

High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

(2013) Kristof Zarschler et al.   Microbial Cell Factories

Nanobodies and their potential applications

(2013) Gholamreza Hassanzadeh-Ghassabeh et al.   Nanomedicine

Bioorthogonal labelling of biomolecules: new functional handles and ligation methods

(2013) Marjoke F. Debets et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification

(2013) Tiana D. Warren et al.   PROTEIN EXPRESSION AND PURIFICATION

Nanobodies Targeting Mouse/Human VCAM1 for the Nuclear Imaging of Atherosclerotic Lesions

(2012) Alexis Broisat et al.   CIRCULATION RESEARCH

Recent contributions in the field of the recombinant expression of disulfide bonded protein in bacteria

(2012) Ario de Marco   Microbial Cell Factories

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

(2012) Kathrin Lang et al.   Nature Chemistry

A novel ligand delivery system to non-invasively visualize and therapeutically exploit the IL13Rα2 tumor-restricted biomarker

(2012) Van Nguyen et al.   NEURO-ONCOLOGY

Pre-diagnostic levels of adiponectin and soluble vascular cell adhesion molecule-1 are associated with colorectal cancer risk

(2012) Mathilde Touvier   WORLD JOURNAL OF GASTROENTEROLOGY

Bioconjugation with Strained Alkenes and Alkynes

(2011) Marjoke F. Debets et al.   ACCOUNTS OF CHEMICAL RESEARCH

Introducing Bioorthogonal Functionalities into Proteins in Living Cells

(2011) Ziyang Hao et al.   ACCOUNTS OF CHEMICAL RESEARCH

Piperidine Acetic Acid Based γ-Secretase Modulators Directly Bind to Presenilin-1

(2011) Christina J. Crump et al.   ACS Chemical Neuroscience

Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study

(2011) Christen Besanceney-Webler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Simultaneous “One Pot” Expressed Protein Ligation and CuI-Catalyzed Azide/Alkyne Cycloaddition for Protein Immobilization

(2011) Max Steinhagen et al.   CHEMBIOCHEM

Bioorthogonal chemical tagging of protein cholesterylation in living cells

(2011) William P. Heal et al.   CHEMICAL COMMUNICATIONS

Preclinical screening of anti-HER2 nanobodies for molecular imaging of breast cancer

(2011) Ilse Vaneycken et al.   FASEB JOURNAL

A llama-derived gelsolin single-domain antibody blocks gelsolin–G-actin interaction

(2010) Anske Van den Abbeele et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Click Chemistry and Bioorthogonal Reactions: Unprecedented Selectivity in the Labeling of Biological Molecules

(2009) Michael D. Best   BIOCHEMISTRY

Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester

(2009) Sanne W.A. Reulen et al.   BMC BIOTECHNOLOGY

Differential tumor-targeting abilities of three single-domain antibody formats

(2009) Andrea Bell et al.   CANCER LETTERS

Protein structure prediction on the Web: a case study using the Phyre server

(2009) Lawrence A Kelley et al.   Nature Protocols

Cu-Catalyzed Azide−Alkyne Cycloaddition

(2008) Morten Meldal et al.   CHEMICAL REVIEWS

Camelid immunoglobulins and nanobody technology

(2008) S. Muyldermans et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Single-domain antibody fragments with high conformational stability

(2002) Mireille Dumoulin et al.   PROTEIN SCIENCE