Cell-free systems in the new age of synthetic biology

Compartmentalization of an all-E. coliCell-Free Expression System for the Construction of a Minimal Cell

(2016) Filippo Caschera et al.   ARTIFICIAL LIFE

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

(2016) V. Georgi et al.   LAB ON A CHIP

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

(2016) Ashty S. Karim et al.   METABOLIC ENGINEERING

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

(2016) Jonathan Garamella et al.   ACS Synthetic Biology

Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption

(2016) Kei Fujiwara et al.   PLoS One

A bulk sub-femtoliter in vitro compartmentalization system using super-fine electrosprays

(2016) Bineet Sharma et al.   Scientific Reports

Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges

(2015) Yi-Heng Percival Zhang   BIOTECHNOLOGY ADVANCES

Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system

(2015) Yonatan Chemla et al.   BIOTECHNOLOGY AND BIOENGINEERING

Performance benchmarking of four cell-free protein expression systems

(2015) Dejan Gagoski et al.   BIOTECHNOLOGY AND BIOENGINEERING

Conceptual and methodological advances in cell-free directed evolution

(2015) Igor Dodevski et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Characterizing and prototyping genetic networks with cell-free transcription–translation reactions

(2015) Melissa K. Takahashi et al.   METHODS

Robust production of recombinant phosphoproteins using cell-free protein synthesis

(2015) Javin P. Oza et al.   Nature Communications

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding Circuit

(2015) Korbinian Kapsner et al.   ACS Synthetic Biology

Rapid cell-free forward engineering of novel genetic ring oscillators

(2015) Henrike Niederholtmeyer et al.   eLife

Cell-free metabolic engineering: Biomanufacturing beyond the cell

(2014) Quentin M. Dudley et al.   Biotechnology Journal

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system

(2014) Yasuaki Kazuta et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins

(2014) Amrita Singh-Blom et al.   JOURNAL OF BIOTECHNOLOGY

Diversity in the dynamical behaviour of a compartmentalized programmable biochemical oscillator

(2014) Maximilian Weitz et al.   Nature Chemistry

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Improved Cell-Free RNA and Protein Synthesis System

(2014) Jun Li et al.   PLoS One

In vitro Fab display: a cell-free system for IgG discovery

(2014) R. L. Stafford et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Programmable on-chip DNA compartments as artificial cells

(2014) E. Karzbrun et al.   SCIENCE

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair

(2013) Cem Albayrak et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Cell-free synthesis system suitable for disulfide-containing proteins

(2013) Takayoshi Matsuda et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Escherichia coli-based cell free production of flagellin and ordered flagellin display on virus-like particles

(2013) Yuan Lu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Why Cells are Microscopic: A Transport-Time Perspective

(2013) Siowling Soh et al.   Journal of Physical Chemistry Letters

Molecular crowding shapes gene expression in synthetic cellular nanosystems

(2013) Cheemeng Tan et al.   Nature Nanotechnology

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

(2013) James Chappell et al.   NUCLEIC ACIDS RESEARCH

In vitro evolution of  -hemolysin using a liposome display

(2013) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Computational codon optimization of synthetic gene for protein expression

(2012) Bevan Chung et al.   BMC Systems Biology

In Vitro Selection of Highly Modified Cyclic Peptides That Act as Tight Binding Inhibitors

(2012) Yollete V. Guillen Schlippe et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Global analysis of chaperone effects using a reconstituted cell-free translation system

(2012) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplexed in Vivo His-Tagging of Enzyme Pathways for in Vitro Single-Pot Multienzyme Catalysis

(2012) Harris H. Wang et al.   ACS Synthetic Biology

Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline

(2011) Katherine Guild et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Assembly of MreB Filaments on Liposome Membranes: A Synthetic Biology Approach

(2011) Yusuke T. Maeda et al.   ACS Synthetic Biology

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Biofuel production by in vitro synthetic enzymatic pathway biotransformation

(2010) Y-H Percival Zhang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System

(2010) Oleksiy Kovtun et al.   PLoS One

Cell-free synthesis of functional aquaporin Z in synthetic liposomes

(2009) Norman T. Hovijitra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Diversity-based, model-guided construction of synthetic gene networks with predicted functions

(2009) Tom Ellis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based

(2008) Jason R Hillebrecht et al.   BMC BIOTECHNOLOGY

An optimized yeast cell-free system: Sufficient for translation of human papillomavirus 58 L1 mRNA and assembly of virus-like particles

(2008) Xiao Wang et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING