Compartmentalization of an all-E. coliCell-Free Expression System for the Construction of a Minimal Cell

A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system

(2015) Filippo Caschera et al.   METABOLIC ENGINEERING

In Vitro Synthesis of theE. coliSec Translocon from DNA

(2014) Hideaki Matsubayashi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

Cell-free metabolic engineering: Biomanufacturing beyond the cell

(2014) Quentin M. Dudley et al.   Biotechnology Journal

The Evolutionary Enhancement of Genotype-Phenotype Linkages in the Presence of Multiple Copies of Genetic Material

(2014) Keisuke Uno et al.   CHEMBIOCHEM

Evolutionary Bayesian Network design for high dimensional experiments

(2014) Debora Slanzi et al.   CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS

Reconstitution of cytoskeletal protein assemblies for large-scale membrane transformation

(2014) Germán Rivas et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Integration of biological parts toward the synthesis of a minimal cell

(2014) Filippo Caschera et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Liposome display for in vitro selection and evolution of membrane proteins

(2014) Satoshi Fujii et al.   Nature Protocols

The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction

(2014) Brian R. Fritz et al.   NUCLEIC ACIDS RESEARCH

Liposome-Based Liquid Handling Platform Featuring Addition, Mixing, and Aliquoting of Femtoliter Volumes

(2014) Hideaki Shiomi et al.   PLoS One

Stochasticity in Gene Expression in a Cell-Sized Compartment

(2014) Kazuya Nishimura et al.   ACS Synthetic Biology

Environmental Influence on the Evolution of Morphological Complexity in Machines

(2014) Joshua E. Auerbach et al.   PLoS Computational Biology

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

(2014) Christopher W. Coyne et al.   Jove-Journal of Visualized Experiments

A Remarkable Self-Organization Process as the Origin of Primitive Functional Cells

(2013) Pasquale Stano et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

(2013) Zachary Z. Sun et al.   Jove-Journal of Visualized Experiments

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

Coping with complexity: Machine learning optimization of cell-free protein synthesis

(2011) Filippo Caschera et al.   BIOTECHNOLOGY AND BIOENGINEERING

Spontaneous Crowding of Ribosomes and Proteins inside Vesicles: A Possible Mechanism for the Origin of Cell Metabolism

(2011) Tereza Pereira de Souza et al.   CHEMBIOCHEM

Programmed Vesicle Fusion Triggers Gene Expression

(2011) Filippo Caschera et al.   LANGMUIR

Development of an artificial cell, from self-organization to computation and self-reproduction

(2011) Vincent Noireaux et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly of MreB Filaments on Liposome Membranes: A Synthetic Biology Approach

(2011) Yusuke T. Maeda et al.   ACS Synthetic Biology

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Spontaneous Protein Crowding in Liposomes: A New Vista for the Origin of Cellular Metabolism

(2010) Pier Luigi Luisi et al.   CHEMBIOCHEM

Giant Vesicles: Preparations and Applications

(2010) Peter Walde et al.   CHEMBIOCHEM

Automated Discovery of Novel Drug Formulations Using Predictive Iterated High Throughput Experimentation

(2010) Filippo Caschera et al.   PLoS One

Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70

(2010) Jonghyeon Shin et al.   Journal of Biological Engineering

Time-Resolved Tracking of a Minimum Gene Expression System Reconstituted in Giant Liposomes

(2009) Hirohide Saito et al.   CHEMBIOCHEM

Biology under construction: in vitro reconstitution of cellular function

(2009) Allen P. Liu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Macromolecular Crowding and Confinement: Biochemical, Biophysical, and Potential Physiological Consequences

(2008) Huan-Xiang Zhou et al.   Annual Review of Biophysics

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Evolution and self-assembly of protocells

(2008) Ricard V. Solé   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY