Biological applications of xeno nucleic acids

Post-SELEX optimization of aptamers

(2016) Shunxiang Gao et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Structural basis for specific inhibition of Autotaxin by a DNA aptamer

(2016) Kazuki Kato et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for specific inhibition of Autotaxin by a DNA aptamer

(2016) Kazuki Kato et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

siRNAmod: A database of experimentally validated chemically modified siRNAs

(2016) Showkat Ahmad Dar et al.   Scientific Reports

Locked 2′-Deoxy-2′,4′-Difluororibo Modified Nucleic Acids: Thermal Stability, Structural Studies, and siRNA Activity

(2015) Saúl Martínez-Montero et al.   ACS Chemical Biology

An overview of the clinical application of antisense oligonucleotides for RNA-targeting therapies

(2015) Graham McClorey et al.   CURRENT OPINION IN PHARMACOLOGY

Selection of LNA-containing DNA aptamers against recombinant human CD73

(2015) Ida C. Elle et al.   Molecular BioSystems

In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids

(2015) Kenta Hagiwara et al.   Molecular BioSystems

Amido-bridged nucleic acids with small hydrophobic residues enhance hepatic tropism of antisense oligonucleotides in vivo

(2015) Tsuyoshi Yamamoto et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing

(2015) Kazutaka Nishina et al.   Nature Communications

Evaluation of 2’-Deoxy-2’-fluoro Antisense Oligonucleotides for Exon Skipping in Duchenne Muscular Dystrophy

(2015) Silvana M G Jirka et al.   Molecular Therapy-Nucleic Acids

Chemistry, Properties, and in Vitro and in Vivo Applications of 2′-O-Methoxyethyl-4′-thioRNA, a Novel Hybrid Type of Chemically Modified RNA

(2014) Yota Saito et al.   CHEMBIOCHEM

Synthesis and Antisense Properties of 2′-O-(2S-Methoxypropyl)-RNA-Modified Gapmer Antisense Oligonucleotides

(2014) Jinghua Yu et al.   ChemMedChem

Design and evaluation of locked nucleic acid-based splice-switching oligonucleotidesin vitro

(2014) Takenori Shimo et al.   NUCLEIC ACIDS RESEARCH

In Vitro and In Vivo Activity of a Novel Locked Nucleic Acid (LNA)-Inhibitor-miR-221 against Multiple Myeloma Cells

(2014) Maria Teresa Di Martino et al.   PLoS One

Antineoplastic Effect of Decoy Oligonucleotide Derived from MGMT Enhancer

(2014) Tamar Canello et al.   PLoS One

Towards XNA nanotechnology: new materials from synthetic genetic polymers

(2014) Vitor B. Pinheiro et al.   TRENDS IN BIOTECHNOLOGY

Antisense oligonucleotides: modifications and clinical trials

(2014) Vivek K. Sharma et al.   MedChemComm

Nucleic acid therapeutics: basic concepts and recent developments

(2014) Vivek K. Sharma et al.   RSC Advances

What Can We Learn From Clinical Trials of Exon Skipping for DMD?

(2014) Qi-long Lu et al.   Molecular Therapy-Nucleic Acids

Capillary Electrophoresis–Systematic Evolution of Ligands by Exponential Enrichment Selection of Base- and Sugar-Modified DNA Aptamers: Target Binding Dominated by 2′-O,4′-C-Methylene-Bridged/Locked Nucleic Acid Primer

(2013) Yuuya Kasahara et al.   ANALYTICAL CHEMISTRY

2′,4′-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides

(2013) Yuuya Kasahara et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Mipomersen Sodium: First Global Approval

(2013) Philip Hair et al.   DRUGS

XNA marks the spot

(2013) Philip Hunter   EMBO REPORTS

Locked nucleic acid antisense inhibitor targeting apolipoprotein C-III efficiently and preferentially removes triglyceride from large very low-density lipoprotein particles in murine plasma

(2013) Tsuyoshi Yamamoto et al.   EUROPEAN JOURNAL OF PHARMACOLOGY

In vitro optimization of 2′-OMe-4′-thioribonucleoside–modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle

(2013) Mayumi Takahashi et al.   NUCLEIC ACIDS RESEARCH

G-rich VEGF aptamer with locked and unlocked nucleic acid modifications exhibits a unique G-quadruplex fold

(2013) Maja Marušič et al.   NUCLEIC ACIDS RESEARCH

Miravirsen (SPC3649) can inhibit the biogenesis of miR-122

(2013) Luca F. R. Gebert et al.   NUCLEIC ACIDS RESEARCH

Effective Exon Skipping and Dystrophin Restoration by 2′-O-Methoxyethyl Antisense Oligonucleotide in Dystrophin-Deficient Mice

(2013) Lu Yang et al.   PLoS One

5′ Unlocked Nucleic Acid Modification Improves siRNA Targeting

(2013) Nicholas M Snead et al.   Molecular Therapy-Nucleic Acids

Inhibition of IL-1 Signaling by Antisense Oligonucleotide-mediated Exon Skipping of IL-1 Receptor Accessory Protein (IL-1RAcP)

(2013) A Seda Yılmaz-Eliş et al.   Molecular Therapy-Nucleic Acids

Amido-Bridged Nucleic Acids (AmNAs): Synthesis, Duplex Stability, Nuclease Resistance, and in Vitro Antisense Potency

(2012) Aiko Yahara et al.   CHEMBIOCHEM

Designing Chemically Modified Oligonucleotides for Targeted Gene Silencing

(2012) Glen F. Deleavey et al.   CHEMISTRY & BIOLOGY

The XNA world: progress towards replication and evolution of synthetic genetic polymers

(2012) Vitor B Pinheiro et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Towards the Sequence-Selective Recognition of Double-Stranded DNA Containing Pyrimidine-Purine Interruptions by Triplex-Forming Oligonucleotides

(2012) Yoshiyuki Hari et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Synthesis and Antisense Properties of Fluoro Cyclohexenyl Nucleic Acid (F-CeNA), a Nuclease Stable Mimic of 2′-Fluoro RNA

(2012) Punit P. Seth et al.   JOURNAL OF ORGANIC CHEMISTRY

Nucleoside Triphosphates — Building Blocks for the Modification of Nucleic Acids

(2012) Marcel Hollenstein   MOLECULES

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Synthetic oligonucleotides recruit ILF2/3 to RNA transcripts to modulate splicing

(2012) Frank Rigo et al.   Nature Chemical Biology

Darwinian evolution of an alternative genetic system provides support for TNA as an RNA progenitor

(2012) Hanyang Yu et al.   Nature Chemistry

Gene silencing by chemically modified siRNAs

(2012) Joachim W. Engels   New Biotechnology

In vivo screening of modified siRNAs for non-specific antiviral effect in a small fish model: number and localization in the strands are important

(2012) Brian Dall Schyth et al.   NUCLEIC ACIDS RESEARCH

TricycloDNA-modified oligo-2′-deoxyribonucleotides reduce scavenger receptor B1 mRNA in hepatic and extra-hepatic tissues—a comparative study of oligonucleotide length, design and chemistry

(2012) Sue Murray et al.   NUCLEIC ACIDS RESEARCH

Intracellular stability of 2′-OMe-4′-thioribonucleoside modified siRNA leads to long-term RNAi effect

(2012) Mayumi Takahashi et al.   NUCLEIC ACIDS RESEARCH

An efficient antigene activity and antiproliferative effect by targeting the Bcl-2 or survivin gene with triplex forming oligonucleotides containing a W-shaped nucleoside analogue (WNA-βT)

(2012) Yosuke Taniguchi et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A short adaptive path from DNA to RNA polymerases

(2012) C. Cozens et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Therapeutic inhibition of the miR-34 family attenuates pathological cardiac remodeling and improves heart function

(2012) B. C. Bernardo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic Genetic Polymers Capable of Heredity and Evolution

(2012) V. B. Pinheiro et al.   SCIENCE

Structure Activity Relationships of α-L-LNA Modified Phosphorothioate Gapmer Antisense Oligonucleotides in Animals

(2012) Punit P Seth et al.   Molecular Therapy-Nucleic Acids

Antisense 2′-Deoxy, 2′-Fluoroarabino Nucleic Acid (2′F-ANA) Oligonucleotides: In Vitro Gymnotic Silencers of Gene Expression Whose Potency Is Enhanced by Fatty Acids

(2012) Naira Souleimanian et al.   Molecular Therapy-Nucleic Acids

Development of a 2′,4′-BNA/LNA-based siRNA for Dyslipidemia and Assessment of the Effects of Its Chemical Modifications In Vivo

(2012) Shunsuke Wada et al.   Molecular Therapy-Nucleic Acids

Cholesterol-lowering Action of BNA-based Antisense Oligonucleotides Targeting PCSK9 in Atherogenic Diet-induced Hypercholesterolemic Mice

(2012) Tsuyoshi Yamamoto et al.   Molecular Therapy-Nucleic Acids

Unique Gene-Silencing and Structural Properties of 2′-Fluoro-Modified siRNAs

(2011) Muthiah Manoharan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Thermodynamic and biological evaluation of a thrombin binding aptamer modified with several unlocked nucleic acid (UNA) monomers and a 2′-C-piperazino-UNA monomer

(2011) Troels B. Jensen et al.   BIOORGANIC & MEDICINAL CHEMISTRY

An Overview of Sugar-Modified Oligonucleotides for Antisense Therapeutics

(2011) Thazha P. Prakash   CHEMISTRY & BIODIVERSITY

Management of CNV in Angioid Streaks by Intravitreal Use of Specific Anti-VEGF165 Aptamer (Pegaptanib Sodium): Long-Term Results

(2011) Osman Çekiç et al.   CURRENT EYE RESEARCH

CRISPR-based adaptive immune systems

(2011) Michael P Terns et al.   CURRENT OPINION IN MICROBIOLOGY

Antisense drug discovery and development

(2011) Tsuyoshi Yamamoto et al.   Future Medicinal Chemistry

Chemical modification and design of anti-miRNA oligonucleotides

(2011) K A Lennox et al.   GENE THERAPY

Emission Wavelength Prediction of a Full-Color-Tunable Fluorescent Core Skeleton, 9-Aryl-1,2-dihydropyrrolo[3,4-b]indolizin-3-one

(2011) Eunha Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

PCSK9 LNA Antisense Oligonucleotides Induce Sustained Reduction of LDL Cholesterol in Nonhuman Primates

(2011) Marie W Lindholm et al.   MOLECULAR THERAPY

Silencing of microRNA families by seed-targeting tiny LNAs

(2011) Susanna Obad et al.   NATURE GENETICS

Unlocked nucleic acid – an RNA modification with broad potential

(2011) Anna Pasternak et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Xenobiology: A new form of life as the ultimate biosafety tool

(2010) Markus Schmidt   BIOESSAYS

RNA interference with 2′,4′-bridged nucleic acid analogues

(2010) S.M. Abdur Rahman et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Effect of 3′-end capping of aptamer with various 2′,4′-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

(2010) Yuuya Kasahara et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Locked Nucleic Acids: Promising Nucleic Acid Analogs for Therapeutic Applications

(2010) Rakesh N. Veedu et al.   CHEMISTRY & BIODIVERSITY

Bridged Nucleic Acids: Development, Synthesis and Properties

(2010) Satoshi Obika et al.   HETEROCYCLES

Configuration of the 5′-Methyl Group Modulates the Biophysical and Biological Properties of Locked Nucleic Acid (LNA) Oligonucleotides

(2010) Punit P. Seth et al.   JOURNAL OF MEDICINAL CHEMISTRY

Antisense Oligonucleotides Containing Conformationally Constrained 2′,4′-(N-Methoxy)aminomethylene and 2′,4′-Aminooxymethylene and 2′-O,4′-C-Aminomethylene Bridged Nucleoside Analogues Show Improved Potency in Animal Models

(2010) Thazha P. Prakash et al.   JOURNAL OF MEDICINAL CHEMISTRY

An Exocyclic Methylene Group Acts As a Bioisostere of the 2′-Oxygen Atom in LNA

(2010) Punit P. Seth et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Recent Progress in Chemically Modified siRNAs

(2010) M. Gaglione et al.   MINI-REVIEWS IN MEDICINAL CHEMISTRY

Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo

(2010) Maria B. Laursen et al.   Molecular BioSystems

Study on Suitability of KOD DNA Polymerase for Enzymatic Production of Artificial Nucleic Acids Using Base/Sugar Modified Nucleoside Triphosphates

(2010) Masayasu Kuwahara et al.   MOLECULES

Aptamers as therapeutics

(2010) Anthony D. Keefe et al.   NATURE REVIEWS DRUG DISCOVERY

Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing

(2010) Glen F. Deleavey et al.   NUCLEIC ACIDS RESEARCH

Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs

(2010) Narendra Vaish et al.   NUCLEIC ACIDS RESEARCH

Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer

(2010) Anna Pasternak et al.   NUCLEIC ACIDS RESEARCH

A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

(2010) Jesper B. Bramsen et al.   NUCLEIC ACIDS RESEARCH

Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates

(2010) Ellen Marie Straarup et al.   NUCLEIC ACIDS RESEARCH

A Direct Comparison of Anti-microRNA Oligonucleotide Potency

(2010) Kim A. Lennox et al.   PHARMACEUTICAL RESEARCH

Application of small interfering RNAs modified by unlocked nucleic acid (UNA) to inhibit the heart-pathogenic coxsackievirus B3

(2009) Denise Werk et al.   FEBS LETTERS

Locked Nucleic Acid (LNA)-Modified Dinucleotide mRNA Cap Analogue: Synthesis, Enzymatic Incorporation, and Utilization

(2009) Anilkumar R. Kore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthesis and characterization of 2′-modified-4′-thioRNA: a comprehensive comparison of nuclease stability

(2009) Mayumi Takahashi et al.   NUCLEIC ACIDS RESEARCH

Analysis of acyclic nucleoside modifications in siRNAs finds sensitivity at position 1 that is restored by 5′-terminal phosphorylation both in vitro and in vivo

(2009) Denise M. Kenski et al.   NUCLEIC ACIDS RESEARCH

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity

(2009) Jesper B. Bramsen et al.   NUCLEIC ACIDS RESEARCH

2′F-Arabinonucleic acids (2′F-ANA) — History, properties, and new frontiers

(2008) Jonathan K Watts et al.   CANADIAN JOURNAL OF CHEMISTRY

Chemically modified siRNA: tools and applications

(2008) J WATTS et al.   DRUG DISCOVERY TODAY

Short Antisense Oligonucleotides with Novel 2′−4′ Conformationaly Restricted Nucleoside Analogues Show Improved Potency without Increased Toxicity in Animals

(2008) Punit P. Seth et al.   JOURNAL OF MEDICINAL CHEMISTRY

Polymerase Chain Reaction and Transcription Using Locked Nucleic Acid Nucleotide Triphosphates

(2008) Rakesh N. Veedu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2′,4′-bridged nucleosides

(2008) Masayasu Kuwahara et al.   NUCLEIC ACIDS RESEARCH