A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo

(2010) Maria B. Laursen et al.   Molecular BioSystems

UNA (unlocked nucleic acid): A flexible RNA mimic that allows engineering of nucleic acid duplex stability

(2009) Niels Langkjær et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Application of small interfering RNAs modified by unlocked nucleic acid (UNA) to inhibit the heart-pathogenic coxsackievirus B3

(2009) Denise Werk et al.   FEBS LETTERS

Analysis of acyclic nucleoside modifications in siRNAs finds sensitivity at position 1 that is restored by 5′-terminal phosphorylation both in vitro and in vivo

(2009) Denise M. Kenski et al.   NUCLEIC ACIDS RESEARCH

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity

(2009) Jesper B. Bramsen et al.   NUCLEIC ACIDS RESEARCH

Off-target effects of siRNA specific for GFP

(2008) Cordula Tschuch et al.   BMC MOLECULAR BIOLOGY

Preparation of Zwitterionic Ribonucleoside Phosphoramidites for Solid-Phase siRNA Synthesis

(2008) Romualdas Smicius et al.   JOURNAL OF ORGANIC CHEMISTRY

The impact of microRNAs on protein output

(2008) Daehyun Baek et al.   NATURE

Widespread changes in protein synthesis induced by microRNAs

(2008) Matthias Selbach et al.   NATURE

Structure of the guide-strand-containing argonaute silencing complex

(2008) Yanli Wang et al.   NATURE

Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect

(2008) Kumiko Ui-Tei et al.   NUCLEIC ACIDS RESEARCH

Synthesis of 2′-O-modified adenosine building blocks and application for RNA interference

(2007) Dalibor Odadzic et al.   BIOORGANIC & MEDICINAL CHEMISTRY