Three-dimensional cell culture models for anticancer drug screening: Worth the effort?

In-depth quantitative analysis and comparison of the human hepatocyte and hepatoma cell line HepG2 proteomes

(2016) Jacek R. Wiśniewski et al.   Journal of Proteomics

Dual-crosslinked hydrogel microwell system for formation and culture of multicellular human adipose tissue-derived stem cell spheroids

(2016) Oju Jeon et al.   Journal of Materials Chemistry B

Characterization of a functional C3A liver spheroid model

(2016) Harriet Gaskell et al.   Toxicology Research

Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment

(2016) Su-Yeong Jeong et al.   PLoS One

Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression

(2016) Sharada Sawant et al.   PLoS One

A paper-based invasion assay: Assessing chemotaxis of cancer cells in gradients of oxygen

(2015) Bobak Mosadegh et al.   BIOMATERIALS

On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro

(2015) Mi Jang et al.   Biomicrofluidics

Microfluidic 3D cell culture: from tools to tissue models

(2015) Vincent van Duinen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Co-culture of 3D tumor spheroids with fibroblasts as a model for epithelial–mesenchymal transition in vitro

(2015) Sun-Ah Kim et al.   EXPERIMENTAL CELL RESEARCH

Challenges and opportunities toward enabling phenotypic screening of complex and 3D cell models

(2015) Shane R Horman et al.   Future Medicinal Chemistry

Three-Dimensional Cell Culture: A Breakthrough in Vivo

(2015) Delphine Antoni et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

An analysis of the attrition of drug candidates from four major pharmaceutical companies

(2015) Michael J. Waring et al.   NATURE REVIEWS DRUG DISCOVERY

The $2.6 Billion Pill — Methodologic and Policy Considerations

(2015) Jerry Avorn   NEW ENGLAND JOURNAL OF MEDICINE

Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation

(2015) Asaf Rotem et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

3D cell culture systems modeling tumor growth determinants in cancer target discovery

(2014) Claudio R. Thoma et al.   ADVANCED DRUG DELIVERY REVIEWS

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Caenorhabditis elegans: A Model System for Anti-Cancer Drug Discovery and Therapeutic Target Identification

(2014) Robert A. Kobet et al.   Biomolecules & Therapeutics

3D Cell Culture Systems: Advantages and Applications

(2014) Maddaly Ravi et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatotoxicity

(2014) Kohei Aritomi et al.   JOURNAL OF PHARMACOLOGICAL SCIENCES

Co-culture systems and technologies: taking synthetic biology to the next level

(2014) L. Goers et al.   Journal of the Royal Society Interface

Quantification of Dynamic Morphological Drug Responses in 3D Organotypic Cell Cultures by Automated Image Analysis

(2014) Ville Härmä et al.   PLoS One

Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

(2014) Delyan P. Ivanov et al.   PLoS One

The efficacy of cetuximab in a tissue-engineered three-dimensional in vitro model of colorectal cancer

(2014) Tarig Magdeldin et al.   Journal of Tissue Engineering

Effects of hypoxia on human cancer cell line chemosensitivity

(2013) Sara Strese et al.   BMC CANCER

Physicochemical regulation of endothelial sprouting in a 3D microfluidic angiogenesis model

(2013) Scott S. Verbridge et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

A novel three-dimensional stromal-based model forin vitrochemotherapy sensitivity testing of leukemia cells

(2013) Omar S. Aljitawi et al.   LEUKEMIA & LYMPHOMA

Three-dimensional cell culturing by magnetic levitation

(2013) William L Haisler et al.   Nature Protocols

Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines

(2013) Anna C. Luca et al.   PLoS One

Multi-cell type human liver microtissues for hepatotoxicity testing

(2012) S. Messner et al.   ARCHIVES OF TOXICOLOGY

Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

(2012) Maria Vinci et al.   BMC BIOLOGY

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

(2012) Brendon M. Baker et al.   JOURNAL OF CELL SCIENCE

Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy

(2012) Geeta Mehta et al.   JOURNAL OF CONTROLLED RELEASE

The advantages of co-culture over mono cell culture in simulating in vivo environment

(2012) Yasuhiro Miki et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Vasopressin/Oxytocin-Related Signaling Regulates Gustatory Associative Learning in C. elegans

(2012) I. Beets et al.   SCIENCE

Optimizing a 3D Culture System to Study the Interaction between Epithelial Breast Cancer and Its Surrounding Fibroblasts

(2012) Liyuan Li et al.   Journal of Cancer

The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation

(2011) Yukie Yoshii et al.   BIOMATERIALS

In vitro three-dimensional (3D) models in cancer research: An update

(2011) Lauren C. Kimlin et al.   MOLECULAR CARCINOGENESIS

Calcium alginate microcapsules with spherical liquid cores templated by gelatin microparticles for mass production of multicellular spheroids

(2010) Shinji Sakai et al.   Acta Biomaterialia

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

Bioengineered 3D platform to explore cell–ECM interactions and drug resistance of epithelial ovarian cancer cells

(2010) Daniela Loessner et al.   BIOMATERIALS

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Preclinical development of molecular-targeted agents for cancer

(2010) Alberto Ocana et al.   Nature Reviews Clinical Oncology

Matrigel Improves Functional Properties of Primary Human Salivary Gland Cells

(2010) Ola M. Maria et al.   TISSUE ENGINEERING PART A

Hydrogels as extracellular matrix mimics for 3D cell culture

(2009) Mark W. Tibbitt et al.   BIOTECHNOLOGY AND BIOENGINEERING

Antiangiogenic Therapy Elicits Malignant Progression of Tumors to Increased Local Invasion and Distant Metastasis

(2009) Marta Pàez-Ribes et al.   CANCER CELL

Preclinical activity of palifosfamide lysine (ZIO-201) in pediatric sarcomas including oxazaphosphorine-resistant osteosarcoma

(2009) Pooja Hingorani et al.   CANCER CHEMOTHERAPY AND PHARMACOLOGY

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem Cells

(2008) Sendurai A. Mani et al.   CELL

Cytochrome P450 and Chemical Toxicology

(2007) F. Peter Guengerich   CHEMICAL RESEARCH IN TOXICOLOGY