Dual modification of biomolecules

Site-Specific Difunctionalization of Structured RNAs Yields Probes for microRNA Maturation

(2016) Ugo Pradère et al.   BIOCONJUGATE CHEMISTRY

Development of Solid-Phase Site-Specific Conjugation and Its Application toward Generation of Dual Labeled Antibody and Fab Drug Conjugates

(2016) Sujiet Puthenveetil et al.   BIOCONJUGATE CHEMISTRY

Dual Unnatural Amino Acid Incorporation and Click-Chemistry Labeling to Enable Single-Molecule FRET Studies of p97 Folding

(2016) Taehyung C. Lee et al.   CHEMBIOCHEM

Chemical Protein Modification through Cysteine

(2016) Smita B. Gunnoo et al.   CHEMBIOCHEM

Erratum: Corrigendum: Recent advances in the construction of antibody–drug conjugates

(2016) Vijay Chudasama et al.   Nature Chemistry

Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides

(2016) Tao Wang et al.   Chemical Science

Next-generation disulfide stapling: reduction and functional re-bridging all in one

(2016) Maximillian T. W. Lee et al.   Chemical Science

The use of 3,6-pyridazinediones in organic synthesis and chemical biology

(2016) Maximillian T. W. Lee et al.   JOURNAL OF CHEMICAL RESEARCH

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

(2015) Michaela Mühlberg et al.   Beilstein Journal of Organic Chemistry

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates

(2015) Atsushi Yamaguchi et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugates: The Nexus of Bioorthogonal Chemistry, Protein Engineering, and Drug Development

(2015) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

A mild TCEP-based para-azidobenzyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates

(2015) Antoine Maruani et al.   CHEMICAL COMMUNICATIONS

Site-selective multi-porphyrin attachment enables the formation of a next-generation antibody-based photodynamic therapeutic

(2015) Antoine Maruani et al.   CHEMICAL COMMUNICATIONS

A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

(2015) Jessica A. Stewart et al.   CHEMICAL COMMUNICATIONS

Advances in Chemical Protein Modification

(2015) Omar Boutureira et al.   CHEMICAL REVIEWS

Molecular features of the sortase enzyme family

(2015) William J. Bradshaw et al.   FEBS Journal

Development of Bioorthogonal Reactions and Their Applications in Bioconjugation

(2015) Mengmeng Zheng et al.   MOLECULES

A plug-and-play approach to antibody-based therapeutics via a chemoselective dual click strategy

(2015) Antoine Maruani et al.   Nature Communications

Bioorthogonal Reactions for Labeling Proteins

(2014) Kathrin Lang et al.   ACS Chemical Biology

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

(2014) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Dual Labeling of Proteins by Using Small Orthogonal Tags at Neutral pH

(2014) Jan Grünewald et al.   CHEMBIOCHEM

A rapid, site-selective and efficient route to the dual modification of DARPins

(2014) Paul Moody et al.   CHEMICAL COMMUNICATIONS

Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation

(2014) Craig S. McKay et al.   CHEMISTRY & BIOLOGY

SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS AND THEIR APPLICATIONS TO THERAPEUTIC ANTIBODIES

(2014) Remko van Vught et al.   Computational and Structural Biotechnology Journal

Chemical Synthesis of Mono- and Bis-Labeled Pre-MicroRNAs

(2013) Ugo Pradère et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Efficient Access to 3′-Terminal Azide-Modified RNA for Inverse Click-Labeling Patterns

(2013) Tobias Santner et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic Bio-orthogonal Chemistry for Site-Specific Double Modification of Recombinant Thrombomodulin

(2013) Rui Jiang et al.   CHEMBIOCHEM

Site-selective three-component reaction for dual-functionalization of peptides

(2013) Henrik K. Munch et al.   CHEMICAL COMMUNICATIONS

Simultaneous Dual Protein Labeling Using a Triorthogonal Reagent

(2013) Mohammad Rashidian et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression

(2013) Andrew Buchanan et al.   mAbs

N-terminal dual protein functionalization by strain-promoted alkyne–nitrone cycloaddition

(2013) Rinske P. Temming et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Bioorthogonal labelling of biomolecules: new functional handles and ligation methods

(2013) Marjoke F. Debets et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

(2013) Ramiz I. Nathani et al.   Chemical Science

Molecular Scaffolds Using Multiple Orthogonal Conjugations: Applications in Chemical Biology and Drug Discovery

(2012) David M. Beal et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific One-Pot Dual Labeling of DNA by Orthogonal Cycloaddition Chemistry

(2012) Juliane Schoch et al.   BIOCONJUGATE CHEMISTRY

Catalyst-Free and Site-Specific One-Pot Dual-Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

(2012) Bo Wu et al.   CHEMBIOCHEM

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

(2012) Montserrat Shelbourne et al.   CHEMICAL COMMUNICATIONS

Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes

(2012) I. Kira Astakhova et al.   CHEMISTRY-A EUROPEAN JOURNAL

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

(2012) Yuichi Minato et al.   JOURNAL OF BIOMOLECULAR NMR

Performance Analysis of Orthogonal Pairs Designed for an Expanded Eukaryotic Genetic Code

(2012) Sebastian Nehring et al.   PLoS One

A “Tag-and-Modify” Approach to Site-Selective Protein Modification

(2011) Justin M. Chalker et al.   ACCOUNTS OF CHEMICAL RESEARCH

Access to Biomolecular Assemblies through One-Pot Triple Orthogonal Chemoselective Ligations

(2011) Mathieu Galibert et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study

(2011) Christen Besanceney-Webler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Facile Double-Functionalization of Designed Ankyrin Repeat Proteins using Click and Thiol Chemistries

(2011) Manuel Simon et al.   BIOCONJUGATE CHEMISTRY

Bromopyridazinedione-mediated protein and peptide bioconjugation

(2011) Vijay Chudasama et al.   CHEMICAL COMMUNICATIONS

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins

(2011) Lucia De Rosa et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Click-enabled heterotrifunctional template for sequential bioconjugations

(2011) David M. Beal et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Dual-Surface Modified Virus Capsids for Targeted Delivery of Photodynamic Agents to Cancer Cells

(2010) Nicholas Stephanopoulos et al.   ACS Nano

Nucleic acid-based fluorescent probes and their analytical potential

(2010) Bernard Juskowiak   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Two-Chain Insulin from a Single-Chain Branched Depsipeptide Precursor: The End of a Long Journey

(2010) Hans-Jürgen Musiol et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Multivalent Display and Receptor-Mediated Endocytosis of Transferrin on Virus-Like Particles

(2010) Deboshri Banerjee et al.   CHEMBIOCHEM

Click chemistry with DNA

(2010) Afaf H. El-Sagheer et al.   CHEMICAL SOCIETY REVIEWS

Vinyl Sulfone Bifunctional Tag Reagents for Single-Point Modification of Proteins

(2010) Julia Morales-Sanfrutos et al.   JOURNAL OF ORGANIC CHEMISTRY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Codon reassignment in the Escherichia coli genetic code

(2010) T. Mukai et al.   NUCLEIC ACIDS RESEARCH

A universal and ready-to-use heterotrifunctional cross-linking reagent for facile synthetic access to sophisticated bioconjugates

(2010) Guillaume Clavé et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A Dual-Mode Fluorescence Strategy for Screening HAT Modulators

(2009) Nan Xie et al.   BIOCONJUGATE CHEMISTRY

Growing Applications of “Click Chemistry” for Bioconjugation in Contemporary Biomedical Research

(2009) Kido Nwe et al.   CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS

Expanding the Scope of ProteinTrans-Splicing to Fragment Ligation of an Integral Membrane Protein: Towards Modulation of Porin-Based Ion Channels by Chemical Modification

(2009) Steffen Brenzel et al.   CHEMBIOCHEM

Synthesis of Mannose and Galactose Oligonucleotide Conjugates by Bi-click chemistry

(2009) Gwladys Pourceau et al.   JOURNAL OF ORGANIC CHEMISTRY

Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity

(2009) John M. Antos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Specific Two-Color Protein Labeling for FRET Studies Using Split Inteins

(2009) Jin-Yi Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

DNA-based nanostructures for molecular sensing

(2009) Jong Bum Lee et al.   Nanoscale

Self-assembly of a nanoscale DNA box with a controllable lid

(2009) Ebbe S. Andersen et al.   NATURE

Click–Click–Click: Single to Triple Modification of DNA

(2008) Philipp M. E. Gramlich et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Advances in Single-Molecule Fluorescence Methods for Molecular Biology

(2008) Chirlmin Joo et al.   Annual Review of Biochemistry

Probing complexes with single fluorophores: factors contributing to dispersion of FRET in DNA/RNA duplexes

(2008) Dmitry I. Cherny et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer

(2008) Eric M. Brustad et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Conjugation of Nucleosides and Oligonucleotides by [3+2] Cycloaddition

(2007) Anup M. Jawalekar et al.   JOURNAL OF ORGANIC CHEMISTRY