Bioorthogonal labelling of biomolecules: new functional handles and ligation methods

Two-Color Glycan Labeling of Live Cells by a Combination of Diels-Alder and Click Chemistry

(2013) Andrea Niederwieser et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

BODIPY-Tetrazine Derivatives as Superbright Bioorthogonal Turn-on Probes

(2013) Jonathan C. T. Carlson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Organic Chemistry and Applications for Labeling and Engineering in Live-Cell Systems

(2013) Yousuke Takaoka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Imaging Cell Surface Glycosylation in Vivo Using “Double Click” Chemistry

(2013) André A. Neves et al.   BIOCONJUGATE CHEMISTRY

Metabolic Glycan Imaging by Isonitrile-Tetrazine Click Chemistry

(2013) Shaun Stairs et al.   CHEMBIOCHEM

Fluorescent Live-Cell Imaging of Metabolically Incorporated Unnatural Cyclopropene-Mannosamine Derivatives

(2013) Christian M. Cole et al.   CHEMBIOCHEM

Nanobody-Functionalized Polymersomes for Tumor-Vessel Targeting

(2013) Marjoke F. Debets et al.   MACROMOLECULAR BIOSCIENCE

Evaluation of strained alkynes for Cu-free click reaction in live mice

(2013) S.M. van den Bosch et al.   NUCLEAR MEDICINE AND BIOLOGY

Click Nucleic Acid Ligation: Applications in Biology and Nanotechnology

(2012) Afaf H. El-Sagheer et al.   ACCOUNTS OF CHEMICAL RESEARCH

Thiacycloalkynes for Copper-Free Click Chemistry

(2012) Gabriela de Almeida et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded Cyclopropene Directs Rapid, Photoclick-Chemistry-Mediated Protein Labeling in Mammalian Cells

(2012) Zhipeng Yu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Live-Cell Imaging of Cyclopropene Tags with Fluorogenic Tetrazine Cycloadditions

(2012) Jun Yang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction

(2012) Emine Kaya et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific One-Pot Dual Labeling of DNA by Orthogonal Cycloaddition Chemistry

(2012) Juliane Schoch et al.   BIOCONJUGATE CHEMISTRY

Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation

(2012) Annika Borrmann et al.   CHEMBIOCHEM

Nitrile Oxide/Alkyne Cycloadditions - A Credible Platform for Synthesis of Bioinspired Molecules by Metal-Free Molecular Clicking

(2012) Frances Heaney   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Recent Advance in Copper-Free Click Chemistry Using Cyclooctynes, and Applications for Living Systems

(2012) Yoshiyuki Manabe   JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN

Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions

(2012) Kathrin Lang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Polar Dibenzocyclooctynes for Selective Labeling of Extracellular Glycoconjugates of Living Cells

(2012) Frédéric Friscourt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Fluorogenic Probe for the Catalyst-Free Detection of Azide-Tagged Molecules

(2012) Frédéric Friscourt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fluorogenic Azidofluoresceins for Biological Imaging

(2012) Peyton Shieh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functionalized Cyclopropenes As Bioorthogonal Chemical Reporters

(2012) David M. Patterson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Control and Design of Mutual Orthogonality in Bioorthogonal Cycloadditions

(2012) Yong Liang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reactivity of Biarylazacyclooctynones in Copper-Free Click Chemistry

(2012) Chelsea G. Gordon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kinetics studies of rapid strain-promoted [3 + 2]-cycloadditions of nitrones with biaryl-aza-cyclooctynone

(2012) Craig S. McKay et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A Pictet-Spengler ligation for protein chemical modification

(2012) P. Agarwal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diazo compounds as highly tunable reactants in 1,3-dipolar cycloaddition reactions with cycloalkynes

(2012) Nicholas A. McGrath et al.   Chemical Science

Orthogonal, metal-free surface modification by strain-promoted azide–alkyne and nitrile oxide–alkene/alkyne cycloadditions

(2012) Christian Wendeln et al.   Chemical Science

Bioconjugation with Strained Alkenes and Alkynes

(2011) Marjoke F. Debets et al.   ACCOUNTS OF CHEMICAL RESEARCH

From Mechanism to Mouse: A Tale of Two Bioorthogonal Reactions

(2011) Ellen M. Sletten et al.   ACCOUNTS OF CHEMICAL RESEARCH

Biomedical Applications of Tetrazine Cycloadditions

(2011) Neal K. Devaraj et al.   ACCOUNTS OF CHEMICAL RESEARCH

Photoinducible Bioorthogonal Chemistry: A Spatiotemporally Controllable Tool to Visualize and Perturb Proteins in Live Cells

(2011) Reyna K. V. Lim et al.   ACCOUNTS OF CHEMICAL RESEARCH

Site-Specific Conjugation of ScFvs Antibodies to Nanoparticles by Bioorthogonal Strain-Promoted Alkyne-Nitrone Cycloaddition

(2011) Miriam Colombo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Staudinger Ligation as a Method for Bioconjugation

(2011) Sander S. van Berkel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioorthogonal Reaction Pairs Enable Simultaneous, Selective, Multi-Target Imaging

(2011) Mark R. Karver et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthesis and Evaluation of a Series of 1,2,4,5-Tetrazines for Bioorthogonal Conjugation

(2011) Mark R. Karver et al.   BIOCONJUGATE CHEMISTRY

Strain-promoted cycloadditions of cyclic nitrones with cyclooctynes for labeling human cancer cells

(2011) Craig S. McKay et al.   CHEMICAL COMMUNICATIONS

Palladium-mediated site-selective Suzuki–Miyaura protein modification at genetically encoded aryl halides

(2011) Christopher D. Spicer et al.   CHEMICAL COMMUNICATIONS

Synthesis of isoxazoles by hypervalent iodine-induced cycloaddition of nitrile oxides to alkynes

(2011) Anup M. Jawalekar et al.   CHEMICAL COMMUNICATIONS

A Non-Fluorinated Monobenzocyclooctyne for Rapid Copper-Free Click Reactions

(2011) Balázs R. Varga et al.   CHEMISTRY-A EUROPEAN JOURNAL

Click Chemistry: 1,2,3-Triazoles as Pharmacophores

(2011) Sandip G. Agalave et al.   Chemistry-An Asian Journal

A Bioorthogonal Quadricyclane Ligation

(2011) Ellen M. Sletten et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells

(2011) Nan Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Design and Synthesis of Highly Reactive Dienophiles for the Tetrazine–trans-Cyclooctene Ligation

(2011) Michael T. Taylor et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Choosing an effective protein bioconjugation strategy

(2011) Nicholas Stephanopoulos et al.   Nature Chemical Biology

Synthesis of a Fluorogenic Cyclooctyne Activated by Cu-Free Click Chemistry

(2011) John C. Jewett et al.   ORGANIC LETTERS

Development and evaluation of new cyclooctynes for cell surface glycan imaging in cancer cells

(2011) Henning Stöckmann et al.   Chemical Science

Protein Modification by Strain-Promoted Alkyne-Nitrone Cycloaddition

(2010) Xinghai Ning et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells

(2010) Jan Dommerholt et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In Vivo Chemistry for Pretargeted Tumor Imaging in Live Mice

(2010) Raffaella Rossin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioorthogonal Turn-On Probes for Imaging Small Molecules inside Living Cells

(2010) Neal K. Devaraj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Labeling Live Cells by Copper-Catalyzed Alkyne−Azide Click Chemistry

(2010) Vu Hong et al.   BIOCONJUGATE CHEMISTRY

Azide: A Unique Dipole for Metal-Free Bioorthogonal Ligations

(2010) Marjoke F. Debets et al.   CHEMBIOCHEM

Bioorthogonal chemistry: recent progress and future directions

(2010) Reyna K. V. Lim et al.   CHEMICAL COMMUNICATIONS

Solid phase strain promoted “click” modification of DNAvia[3+2]-nitrile oxide–cyclooctyne cycloadditions

(2010) Ishwar Singh et al.   CHEMICAL COMMUNICATIONS

Cu-free click cycloaddition reactions in chemical biology

(2010) John C. Jewett et al.   CHEMICAL SOCIETY REVIEWS

High-Density DNA Functionalization by a Combination of Cu-Catalyzed and Cu-Free Click Chemistry

(2010) Katrin Gutsmiedl et al.   CHEMISTRY-A EUROPEAN JOURNAL

Difluorobenzocyclooctyne: Synthesis, Reactivity, and Stabilization by β-Cyclodextrin

(2010) Ellen M. Sletten et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Olefin Cross-Metathesis on Proteins: Investigation of Allylic Chalcogen Effects and Guiding Principles in Metathesis Partner Selection

(2010) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocompatible Copper(I) Catalysts for in Vivo Imaging of Glycans

(2010) David Soriano del Amo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Cu-Free Click Chemistry with Readily Synthesized Biarylazacyclooctynones

(2010) John C. Jewett et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metal-Free Sequential [3 + 2]-Dipolar Cycloadditions using Cyclooctynes and 1,3-Dipoles of Different Reactivity

(2010) Brian C. Sanders et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A bioorthogonal chemistry strategy for probing protein lipidation in live cells

(2010) Wenjiao Song et al.   Molecular BioSystems

Bioorthogonal chemistry amplifies nanoparticle binding and enhances the sensitivity of cell detection

(2010) Jered B. Haun et al.   Nature Nanotechnology

Visualizing enveloping layer glycans during zebrafish early embryogenesis

(2010) J. M. Baskin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fast Alkene Functionalization In Vivo by Photoclick Chemistry: HOMO Lifting of Nitrile Imine Dipoles

(2009) Yizhong Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Iterative In Situ Click Chemistry Creates Antibody-like Protein-Capture Agents

(2009) Heather D. Agnew et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Analysis and Optimization of Copper-Catalyzed Azide–Alkyne Cycloaddition for Bioconjugation

(2009) Vu Hong et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Nitrones as dipoles for rapid strain-promoted 1,3-dipolar cycloadditions with cyclooctynes

(2009) Craig S. McKay et al.   CHEMICAL COMMUNICATIONS

Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition

(2009) Marjoke F. Debets et al.   CHEMICAL COMMUNICATIONS

Expanding the Chemical Biologists Tool Kit: Chemical Labelling Strategies and Its Applications

(2009) Souvik Chattopadhaya et al.   CURRENT MEDICINAL CHEMISTRY

Thiol-Selective Fluorogenic Probes for Labeling and Release

(2009) Vu Hong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Labeling of Living Cells by a Photo-Triggered Click Reaction

(2009) Andrei A. Poloukhtine et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthesis of N-Terminally Linked Protein Dimers and Trimers by a Combined Native Chemical Ligation-CuAAC Click Chemistry Strategy

(2009) Junpeng Xiao et al.   ORGANIC LETTERS

Copper-Free “Click” Modification of DNA via Nitrile Oxide−Norbornene 1,3-Dipolar Cycloaddition

(2009) Katrin Gutsmiedl et al.   ORGANIC LETTERS

Benzyne Click Chemistry with in Situ Generated Aromatic Azides

(2009) Fengzhi Zhang et al.   ORGANIC LETTERS

A Photoinducible 1,3-Dipolar Cycloaddition Reaction for Rapid, Selective Modification of Tetrazole-Containing Proteins

(2008) Wenjiao Song et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A FRET-Based Fluorogenic Phosphine for Live-Cell Imaging with the Staudinger Ligation

(2008) Matthew J. Hangauer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper-Free and Fast Huisgen Cycloadditions

(2008) Xinghai Ning et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging

(2008) Neal K. Devaraj et al.   BIOCONJUGATE CHEMISTRY

Single-Step Azide Introduction in Proteins via an Aqueous Diazo Transfer

(2008) Stijn F. M. van Dongen et al.   BIOCONJUGATE CHEMISTRY

Application of Metal‐Free Triazole Formation in the Synthesis of Cyclic RGD–DTPA Conjugates

(2008) Sander S. van Berkel et al.   CHEMBIOCHEM

Cu-Catalyzed Azide−Alkyne Cycloaddition

(2008) Morten Meldal et al.   CHEMICAL REVIEWS

Allyl Sulfides Are Privileged Substrates in Aqueous Cross-Metathesis: Application to Site-Selective Protein Modification

(2008) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Second-Generation Difluorinated Cyclooctynes for Copper-Free Click Chemistry

(2008) Julian A. Codelli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tetrazine Ligation: Fast Bioconjugation Based on Inverse-Electron-Demand Diels−Alder Reactivity

(2008) Melissa L. Blackman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Copper-free ‘click’: 1,3-dipolar cycloaddition of azides and arynes

(2008) Lachlan Campbell-Verduyn et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Benzyne Click Chemistry: Synthesis of Benzotriazoles from Benzynes and Azides

(2008) Feng Shi et al.   ORGANIC LETTERS

A Hydrophilic Azacyclooctyne for Cu-Free Click Chemistry

(2008) Ellen M. Sletten et al.   ORGANIC LETTERS

In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish

(2008) S. T. Laughlin et al.   SCIENCE

A smooth access to benzotriazoles via azide-benzyne cycloaddition

(2008) S. Chandrasekhar et al.   TETRAHEDRON