Journal
ANNUAL REVIEW OF BIOCHEMISTRY
Volume 77, Issue -, Pages 51-76Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.biochem.77.070606.101543
Keywords
fluorophore; force spectroscopy; FRET; live cell imaging; polarization; single-particle tracking
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Funding
- NIGMS NIH HHS [R01 GM065367, R01 GM 065367, R01 GM 074526] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM065367, R21GM074526] Funding Source: NIH RePORTER
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Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods have matured and branched out to address numerous biological questions, which were inaccessible via ensemble measurements. Among the current arsenal, SM fluorescence techniques have capabilities of probing the dynamic interactions of nucleic acids and proteins via Forster (fluorescence) resonance energy transfer (FRET), tracking single particles over microns of distances, and deciphering the rotational motion of multisubunit systems. In this exciting era of transitioning from in vitro to in vivo and in situ conditions, it is anticipated that SM fluorescence methodology will become a common tool of molecular biology.
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