Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook

Room-temperature structural studies of SARS-CoV-2 protein NendoU with an X-ray free-electron laser

(2023) Rebecca J. Jernigan et al.   STRUCTURE

Ultrafast structural changes direct the first molecular events of vision

(2023) Thomas Gruhl et al.   NATURE

Structural evidence for intermediates during O2 formation in photosystem II

(2023) Asmit Bhowmick et al.   NATURE

Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography

(2023) Maximilian Wranik et al.   Nature Communications

Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography

(2023) Mohammad Vakili et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser

(2022) Xiaohong Liu et al.   Nature Chemistry

Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease

(2022) Meredith N Frazier et al.   NUCLEIC ACIDS RESEARCH

Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs

(2022) Philipp S. Simon et al.   FEBS LETTERS

Observation of Cation Chromophore Photoisomerization of a Fluorescent Protein Using Millisecond Synchrotron Serial Crystallography and Infrared Vibrational and Visible Spectroscopy

(2022) James M. Baxter et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Time-resolved serial femtosecond crystallography on fatty-acid photodecarboxylase: lessons learned

(2022) Kyprianos Hadjidemetriou et al.   Acta Crystallographica Section D-Structural Biology

Early-stage dynamics of chloride ion–pumping rhodopsin revealed by a femtosecond X-ray laser

(2021) Ji-Hye Yun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism and dynamics of fatty acid photodecarboxylase

(2021) D. Sorigué et al.   SCIENCE

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

(2021) Sebastian Günther et al.   SCIENCE

High-resolution crystal structures of transient intermediates in the phytochrome photocycle

(2021) Melissa Carrillo et al.   STRUCTURE

Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet

(2021) Marie Luise Grünbein et al.   Nature Communications

Capturing structural changes of the S1 to S2 transition of photosystem II using time-resolved serial femtosecond crystallography

(2021) Hongjie Li et al.   IUCrJ

Near-physiological-temperature serial crystallography reveals conformations of SARS-CoV-2 main protease active site for improved drug repurposing

(2021) Serdar Durdagi et al.   STRUCTURE

The three-dimensional structure of Drosophila melanogaster (6–4) photolyase at room temperature

(2021) Andrea Cellini et al.   Acta Crystallographica Section D-Structural Biology

Using photocaging for fast time-resolved structural biology studies

(2021) Diana C. F. Monteiro et al.   Acta Crystallographica Section D-Structural Biology

Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals

(2021) J. Mia Lahey-Rudolph et al.   IUCrJ

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

(2021) Suraj Pandey et al.   IUCrJ

X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis

(2021) Patrick Rabe et al.   Science Advances

Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light

(2021) Mark A. Wilson   Annual Review of Biophysics

Few-fs resolution of a photoactive protein traversing a conical intersection

(2021) A. Hosseinizadeh et al.   NATURE

Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition

(2021) Rana Hussein et al.   Nature Communications

Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I

(2021) Stephen M. Keable et al.   Scientific Reports

Best practices for time-resolved serial synchrotron crystallography

(2021) Eike C. Schulz et al.   Acta Crystallographica Section D-Structural Biology

Case Study of High-Throughput Drug Screening and Remote Data Collection for SARS-CoV-2 Main Protease by Using Serial Femtosecond X-ray Crystallography

(2021) Omur Guven et al.   Crystals

Effects of x-ray free-electron laser pulse intensity on the Mn Kβ1,3 x-ray emission spectrum in photosystem II—A case study for metalloprotein crystals and solutions

(2021) Thomas Fransson et al.   Structural Dynamics-US

Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

(2020) Joyce Woodhouse et al.   Nature Communications

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

(2020) Guillaume Tetreau et al.   Nature Communications

Femtosecond-to-millisecond structural changes in a light-driven sodium pump

(2020) Petr Skopintsev et al.   NATURE

A MHz-repetition-rate hard X-ray free-electron laser driven by a superconducting linear accelerator

(2020) W. Decking et al.   Nature Photonics

Untangling the sequence of events during the S2 → S3 transition in photosystem II and implications for the water oxidation mechanism

(2020) Mohamed Ibrahim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser

(2020) Elin Claesson et al.   eLife

First Experiments in Structural Biology at the European X-ray Free-Electron Laser

(2020) Grant Mills et al.   Applied Sciences-Basel

The ePix10k 2-megapixel hard X-ray detector at LCLS

(2020) Tim Brandt van Driel et al.   JOURNAL OF SYNCHROTRON RADIATION

Tracking Ca2+ ATPase intermediates in real time by x-ray solution scattering

(2020) Harsha Ravishankar et al.   Science Advances

Microfluidic polyimide gas dynamic virtual nozzles for serial crystallography

(2020) Mohammad Vakili et al.   REVIEW OF SCIENTIFIC INSTRUMENTS

Characteristics of SARS-CoV-2 and COVID-19

(2020) Ben Hu et al.   NATURE REVIEWS MICROBIOLOGY

An ultra-compact x-ray free-electron laser

(2020) J B Rosenzweig et al.   NEW JOURNAL OF PHYSICS

Serial synchrotron crystallography for time-resolved structural biology

(2020) Arwen R Pearson et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The XBI BioLab for life science experiments at the European XFEL

(2020) Huijong Han et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Sample delivery for serial crystallography at free-electron lasers and synchrotrons

(2019) Marie Luise Grünbein et al.   Acta Crystallographica Section D-Structural Biology

The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source

(2019) Raymond G. Sierra et al.   JOURNAL OF SYNCHROTRON RADIATION

Strategies for sample delivery for femtosecond crystallography

(2019) Isabelle Martiel et al.   Acta Crystallographica Section D-Structural Biology

Snapshot of an oxygen intermediate in the catalytic reaction of cytochrome c oxidase

(2019) Izumi Ishigami et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microfluidic Mixing Injector Holder Enables Routine Structural Enzymology Measurements with Mix-and-Inject Serial Crystallography Using X-ray Free Electron Lasers

(2019) George D. Calvey et al.   ANALYTICAL CHEMISTRY

Microfluidic sample delivery for serial crystallography using XFELs

(2019) Austin Echelmeier et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin

(2019) Gabriela Nass Kovacs et al.   Nature Communications

Time-resolved serial femtosecond crystallography at the European XFEL

(2019) Suraj Pandey et al.   NATURE METHODS

An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser

(2019) Michihiro Suga et al.   SCIENCE

Membrane protein megahertz crystallography at the European XFEL

(2019) Chris Gisriel et al.   Nature Communications

Time-resolved studies of metalloproteins using X-ray free electron laser radiation at SACLA

(2019) Michihiro Suga et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Mix-and-inject XFEL crystallography reveals gated conformational dynamics during enzyme catalysis

(2019) Medhanjali Dasgupta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Serial Femtosecond Crystallography of G Protein–Coupled Receptors

(2018) Benjamin Stauch et al.   Annual Review of Biophysics

X-ray Emission Spectroscopy as an in Situ Diagnostic Tool for X-ray Crystallography of Metalloproteins Using an X-ray Free-Electron Laser

(2018) Thomas Fransson et al.   BIOCHEMISTRY

Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography

(2018) Jose L. Olmos et al.   BMC BIOLOGY

Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

(2018) Przemyslaw Nogly et al.   SCIENCE

Megahertz data collection from protein microcrystals at an X-ray free-electron laser

(2018) Marie Luise Grünbein et al.   Nature Communications

Megahertz serial crystallography

(2018) Max O. Wiedorn et al.   Nature Communications

De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography

(2018) S. Botha et al.   IUCrJ

Non-cryogenic structure of a chloride pump provides crucial clues to temperature-dependent channel transport efficiency

(2018) Ji-Hye Yun et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structures of the intermediates of Kok’s photosynthetic water oxidation clock

(2018) Jan Kern et al.   NATURE

The Adaptive Gain Integrating Pixel Detector at the European XFEL

(2018) Aschkan Allahgholi et al.   JOURNAL OF SYNCHROTRON RADIATION

X-ray free electron laser: opportunities for drug discovery

(2017) Robert K.Y. Cheng et al.   Essays in Biochemistry

Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography

(2017) J. R. Stagno et al.   NATURE

High-speed fixed-target serial virus crystallography

(2017) Philip Roedig et al.   NATURE METHODS

Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

(2017) Franklin D Fuller et al.   NATURE METHODS

Hard X-ray free-electron laser with femtosecond-scale timing jitter

(2017) Heung-Sik Kang et al.   Nature Photonics

Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

(2017) Cornelius Gati et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pink-beam serial crystallography

(2017) A. Meents et al.   Nature Communications

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

(2017) Tobias Weinert et al.   Nature Communications

Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate

(2017) Takehiko Tosha et al.   Nature Communications

Double-flow focused liquid injector for efficient serial femtosecond crystallography

(2017) Dominik Oberthuer et al.   Scientific Reports

Mix-and-diffuse serial synchrotron crystallography

(2017) Kenneth R. Beyerlein et al.   IUCrJ

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

(2017) Jose M. Martin-Garcia et al.   IUCrJ

Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography

(2017) Kazuya Hasegawa et al.   JOURNAL OF SYNCHROTRON RADIATION

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure

(2016) Jacques-Philippe Colletier et al.   NATURE

Structure of photosystem II and substrate binding at room temperature

(2016) Iris D. Young et al.   NATURE

AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy

(2016) F.X. Kärtner et al.   NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

(2016) K. Pande et al.   SCIENCE

A three-dimensional movie of structural changes in bacteriorhodopsin

(2016) Eriko Nango et al.   SCIENCE

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

(2016) Przemyslaw Nogly et al.   Nature Communications

Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers

(2016) George D. Calvey et al.   Structural Dynamics

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

(2016) Petra Edlund et al.   Scientific Reports

Metalloprotein structures at ambient conditions and in real-time: biological crystallography and spectroscopy using X-ray free electron lasers

(2015) Jan Kern et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

(2015) Yanyong Kang et al.   NATURE

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

(2015) Raymond G Sierra et al.   NATURE METHODS

Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation

(2015) T. R. M. Barends et al.   SCIENCE

A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

(2015) Thomas D. Murray et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams

(2015) Sabine Botha et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams

(2015) Nicolas Coquelle et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

In meso in situserial X-ray crystallography of soluble and membrane proteins

(2015) Chia-Ying Huang et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

(2015) Przemyslaw Nogly et al.   IUCrJ

Serial femtosecond crystallography: the first five years

(2015) Ilme Schlichting   IUCrJ

Characterization and use of the spent beam for serial operation of LCLS

(2015) Sébastien Boutet et al.   JOURNAL OF SYNCHROTRON RADIATION

Demonstration of simultaneous experiments using thin crystal multiplexing at the Linac Coherent Light Source

(2015) Y. Feng et al.   JOURNAL OF SYNCHROTRON RADIATION

Real-time investigation of dynamic protein crystallization in living cells

(2015) R. Schönherr et al.   Structural Dynamics

From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design

(2014) Rolf Hilgenfeld   FEBS Journal

Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

(2014) Christopher Kupitz et al.   NATURE

Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses

(2014) Michihiro Suga et al.   NATURE

Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography

(2014) Wei Liu et al.   Nature Protocols

The Mn4Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser

(2014) R. Tran et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Methods development for diffraction and spectroscopy studies of metalloenzymes at X-ray free-electron lasers

(2014) J. Kern et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Structural biology at the European X-ray free-electron laser facility

(2014) M. Altarelli et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Protein crystal structure obtained at 2.9 A resolution from injecting bacterial cells into an X-ray free-electron laser beam

(2014) M. R. Sawaya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

(2014) Jason Tenboer et al.   SCIENCE

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

(2014) Uwe Weierstall et al.   Nature Communications

Serial crystallography onin vivogrown microcrystals using synchrotron radiation

(2014) Cornelius Gati et al.   IUCrJ

Room-temperature macromolecular serial crystallography using synchrotron radiation

(2014) Francesco Stellato et al.   IUCrJ

Serial Femtosecond Crystallography of G Protein-Coupled Receptors

(2013) W. Liu et al.   SCIENCE

Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature

(2013) J. Kern et al.   SCIENCE

Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography

(2013) Marius Schmidt   Advances in Condensed Matter Physics

A compact X-ray free-electron laser emitting in the sub-ångström region

(2012) Tetsuya Ishikawa et al.   Nature Photonics

Time-resolved protein nanocrystallography using an X-ray free-electron laser

(2012) Andrew Aquila et al.   OPTICS EXPRESS

Injector for scattering measurements on fully solvated biospecies

(2012) U. Weierstall et al.   REVIEW OF SCIENTIFIC INSTRUMENTS

Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser

(2012) L. Redecke et al.   SCIENCE

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

(2012) S. Boutet et al.   SCIENCE

Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination

(2011) Petra Fromme et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Femtosecond X-ray protein nanocrystallography

(2011) Henry N. Chapman et al.   NATURE

First lasing and operation of an ångstrom-wavelength free-electron laser

(2010) P. Emma et al.   Nature Photonics

Gas dynamic virtual nozzle for generation of microscopic droplet streams

(2008) D P DePonte et al.   JOURNAL OF PHYSICS D-APPLIED PHYSICS