Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration

Sustainable manufacturing with synthetic biology

(2022) Corinne D. Scown et al.   NATURE BIOTECHNOLOGY

Engineering synthetic auxotrophs for growth-coupled directed protein evolution

(2022) Jiuzhou Chen et al.   TRENDS IN BIOTECHNOLOGY

gcFront: a tool for determining a Pareto front of growth-coupled cell factory designs

(2022) Laurence Legon et al.   BIOINFORMATICS

Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

(2022) Daniel C. Volke et al.   Nature Communications

Expanding biochemical knowledge and illuminating metabolic dark matter with ATLASx

(2022) Homa MohammadiPeyhani et al.   Nature Communications

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

(2022) Eleni Theodosiou et al.   Frontiers in Bioengineering and Biotechnology

‘The entire protein universe’: AI predicts shape of nearly every known protein

(2022) Ewen Callaway   NATURE

Toward sustainable, cell-free biomanufacturing

(2021) Blake J Rasor et al.   CURRENT OPINION IN BIOTECHNOLOGY

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain

(2021) Madeleine Bouzon et al.   mBio

Growth-coupled selection of synthetic modules to accelerate cell factory development

(2021) Enrico Orsi et al.   Nature Communications

Growth-Based, High-Throughput Selection for NADH Preference in an Oxygen-Dependent Biocatalyst

(2021) Sarah Maxel et al.   ACS Synthetic Biology

Versatile selective evolutionary pressure using synthetic defect in universal metabolism

(2021) Lara Sellés Vidal et al.   Nature Communications

An “energy‐auxotroph” Escherichia coli provides an in vivo platform for assessing NADH regeneration systems

(2020) Sebastian Wenk et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor

(2020) William B. Black et al.   Microbial Cell Factories

A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site

(2020) Sarah Maxel et al.   ACS Catalysis

In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP+

(2020) Liliana Calzadiaz-Ramirez et al.   ACS Catalysis

Engineering Carboxylic Acid Reductase (CAR) through a Whole-Cell Growth-Coupled NADPH Recycling Strategy

(2020) Levi Kramer et al.   ACS Synthetic Biology

In Vivo, High-Throughput Selection of Thermostable Cyclohexanone Monooxygenase (CHMO)

(2020) Sarah Maxel et al.   Catalysts

Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development

(2020) Edward King et al.   CURRENT OPINION IN BIOTECHNOLOGY

Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo

(2020) Hyojin Park et al.   NUCLEIC ACIDS RESEARCH

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

(2020) Beatriz Álvarez et al.   Nature Communications

Structural Insights into Phosphite Dehydrogenase Variants Favoring a Non-natural Redox Cofactor

(2019) Yuxue Liu et al.   ACS Catalysis

Synthetic evolution

(2019) Anna J. Simon et al.   NATURE BIOTECHNOLOGY

Determination of growth-coupling strategies and their underlying principles

(2019) Tobias B. Alter et al.   BMC BIOINFORMATICS

A critical comparison of cellular and cell-free bioproduction systems

(2019) Nico J Claassens et al.   CURRENT OPINION IN BIOTECHNOLOGY

Strategies for optimization of heterologous protein expression in E. coli : Roadblocks and reinforcements

(2018) Jashandeep Kaur et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions

(2018) Jiaheng Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles

(2018) Linyue Zhang et al.   ACS Synthetic Biology

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

Barriers and opportunities in bio-based production of hydrocarbons

(2018) Yongjin J. Zhou et al.   Nature Energy

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

(2018) Arjun Ravikumar et al.   CELL

Selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance

(2017) Keming Liang et al.   METABOLIC ENGINEERING

Growth-coupled overproduction is feasible for almost all metabolites in five major production organisms

(2017) Axel von Kamp et al.   Nature Communications

Fully automatized high-throughput enzyme library screening using a robotic platform

(2016) Mark Dörr et al.   BIOTECHNOLOGY AND BIOENGINEERING

l -Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene

(2016) Seiki Takeno et al.   METABOLIC ENGINEERING

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Fuelling the future: microbial engineering for the production of sustainable biofuels

(2016) James C. Liao et al.   NATURE REVIEWS MICROBIOLOGY

A General Tool for Engineering the NAD/NADP Cofactor Preference of Oxidoreductases

(2016) Jackson K. B. Cahn et al.   ACS Synthetic Biology

Dynamics of NAD-metabolism: everything but constant

(2015) C. A. Opitz et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Advanced technologies for improved expression of recombinant proteins in bacteria: perspectives and applications

(2015) Sanjeev K. Gupta et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Enzyme engineering in biomimetic compartments

(2015) Pierre-Yves Colin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

High-throughput analysis and protein engineering using microcapillary arrays

(2015) Bob Chen et al.   Nature Chemical Biology

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Enhanced acetic acid and succinic acid production under microaerobic conditions by Corynebacterium glutamicum harboring Escherichia coli transhydrogenase gene pntAB

(2014) Yuto Yamauchi et al.   JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY

Metabolic and transcriptional response of Escherichia coli with a NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans

(2013) Sara Centeno-Leija et al.   ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY

Cofactor engineering for advancing chemical biotechnology

(2013) Yipeng Wang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Recombinant protein production in bacterial hosts

(2013) Tim W. Overton   DRUG DISCOVERY TODAY

Bio-based production of C2-C6 platform chemicals

(2012) Yu-Sin Jang et al.   BIOTECHNOLOGY AND BIOENGINEERING

A selection platform for carbon chain elongation using the CoA-dependent pathway to produce linear higher alcohols

(2012) Hidevaldo B. Machado et al.   METABOLIC ENGINEERING

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

(2011) Claire R. Shen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD+-Auxotrophic Mutant

(2011) Yongjin Zhou et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ethanol Production from Xylose by a RecombinantCandida utilisStrain Expressing Protein-Engineered Xylose Reductase and Xylitol Dehydrogenase

(2011) Hideyuki TAMAKAWA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Improved NADPH supply for xylitol production by engineered Escherichia coli with glycolytic mutations

(2011) Jonathan W. Chin et al.   BIOTECHNOLOGY PROGRESS

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

(2011) Sabine Bastian et al.   METABOLIC ENGINEERING

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae

(2009) Oskar Bengtsson et al.   Biotechnology for Biofuels

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering

(2009) Joseph A. Chemler et al.   METABOLIC ENGINEERING

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology

Replacing Escherichia coli NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with a NADP-dependent enzyme from Clostridium acetobutylicum facilitates NADPH dependent pathways

(2008) Irene Martínez et al.   METABOLIC ENGINEERING