Engineering synthetic auxotrophs for growth-coupled directed protein evolution

Engineered dual selection for directed evolution of SpCas9 PAM specificity

(2021) Gregory W. Goldberg et al.   Nature Communications

Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

(2021) Aaron Cravens et al.   Nature Communications

Directed evolution of alditol oxidase for the production of optically pure D-glycerate from glycerol in the engineered Escherichia coli

(2021) Chao Zhang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Growth-Based, High-Throughput Selection for NADH Preference in an Oxygen-Dependent Biocatalyst

(2021) Sarah Maxel et al.   ACS Synthetic Biology

Versatile selective evolutionary pressure using synthetic defect in universal metabolism

(2021) Lara Sellés Vidal et al.   Nature Communications

In vivo directed enzyme evolution in nanoliter reactors with antimetabolite selection

(2020) Christian Femmer et al.   METABOLIC ENGINEERING

An “energy‐auxotroph” Escherichia coli provides an in vivo platform for assessing NADH regeneration systems

(2020) Sebastian Wenk et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site

(2020) Sarah Maxel et al.   ACS Catalysis

In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP+

(2020) Liliana Calzadiaz-Ramirez et al.   ACS Catalysis

Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses

(2019) Hao Luo et al.   PLOS BIOLOGY

Design and engineering of E. coli metabolic sensor strains with a wide sensitivity range for glycerate

(2019) Selçuk Aslan et al.   METABOLIC ENGINEERING

Growth-coupled evolution of phosphoketolase to improve l-glutamate production by Corynebacterium glutamicum

(2019) Taiwo Dele-Osibanjo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY