Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development

NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

(2020) Alina Spielmann et al.   AMB Express

Engineering D‐lactate dehydrogenase to favor a non‐natural cofactor nicotinamide cytosine dinucleotide

(2020) Yuxue Liu et al.   CHEMBIOCHEM

Enzymatic Preparation of the Chiral (S)-Sulfoxide Drug Esomeprazole at Pilot-Scale Levels

(2020) Na Xu et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Molecular Dynamics Analysis of a Rationally Designed Aldehyde Dehydrogenase Gives Insights into Improved Activity for the Non-Native Cofactor NAD+

(2020) Tobias J. Gmelch et al.   ACS Synthetic Biology

A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site

(2020) Sarah Maxel et al.   ACS Catalysis

In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP+

(2020) Liliana Calzadiaz-Ramirez et al.   ACS Catalysis

Engineering of a chromogenic enzyme screening system based on an auxiliary indole-3-carboxylic acid monooxygenase

(2019) Vida Časaitė et al.   MicrobiologyOpen

Engineering of Cyclohexanone Monooxygenase for the Enantioselective Synthesis of (S)-Omeprazole

(2019) Yan Zhang et al.   ACS Sustainable Chemistry & Engineering

Structural Insights into Phosphite Dehydrogenase Variants Favoring a Non-natural Redox Cofactor

(2019) Yuxue Liu et al.   ACS Catalysis

Convergent cascade catalyzed by monooxygenase - alcohol dehydrogenase fusion applied in organic media

(2019) Lei Huang et al.   CHEMBIOCHEM

A high-throughput mass spectrometric enzyme activity assay enabling the discovery of cytochrome P450 biocatalysts

(2019) Tristan de Rond et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of a Cytochrome P450 Carbene Transferase for Selective Functionalization of Cyclic Compounds

(2019) Oliver F. Brandenberg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrahigh-throughput screening enables efficient single-round oxidase remodelling

(2019) Aaron Debon et al.   Nature Catalysis

Engineering a nicotinamide mononucleotide redox cofactor system for biocatalysis

(2019) William B. Black et al.   Nature Chemical Biology

A High-Throughput Method for Directed Evolution of NAD(P)+-Dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues

(2019) Rui Huang et al.   ACS Catalysis

Non-natural Cofactor and Formate-Driven Reductive Carboxylation of Pyruvate

(2019) Xiaojia Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Flavoenzyme‐mediated Regioselective Aromatic Hydroxylation with Coenzyme Biomimetics

(2019) Alice Guarneri et al.   ChemCatChem

Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application

(2018) Lara Sellés Vidal et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A Chimeric Styrene Monooxygenase with Increased Efficiency in Asymmetric Biocatalytic Epoxidation

(2018) Maria L. Corrado et al.   CHEMBIOCHEM

Creation of a formate: malate oxidoreductase by fusion of dehydrogenase enzymes with PEGylated cofactor swing arms

(2018) Harun F Ozbakir et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Insertion of a Calcium-Responsive β-Roll Domain into a Thermostable Alcohol Dehydrogenase Enables Tunable Control over Cofactor Selectivity

(2018) Walaa Abdallah et al.   ACS Catalysis

An Engineered Self-Sufficient Biocatalyst Enables Scalable Production of Linear α-Olefins from Carboxylic Acids

(2018) Chen Lu et al.   ACS Catalysis

Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles

(2018) Linyue Zhang et al.   ACS Synthetic Biology

Protein Engineering for Nicotinamide Coenzyme Specificity in Oxidoreductases: Attempts and Challenges

(2018) Andrea M. Chánique et al.   Frontiers in Microbiology

A hydroquinone-specific screening system for directed P450 evolution

(2018) Alexandra M. Weingartner et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Design of artificial alcohol oxidases: alcohol dehydrogenase - NADPH oxidase fusions for continuous oxidations

(2018) Friso Aalbers et al.   CHEMBIOCHEM

NADPH-Auxotrophic E. coli: A Sensor Strain for Testing in Vivo Regeneration of NADPH

(2018) Steffen N. Lindner et al.   ACS Synthetic Biology

Bioorganometallic chemistry: Co-factor regeneration, enzyme recognition of biomimetic 1,4-NADH analogs, and organic synthesis; tandem catalyzed regioselective formation of N-substituted-1,4-dihydronicotinamide derivatives with [Cp*Rh(bpy)H] + , coupled to chiral S-alcohol formation with HLADH, and engineered cytochrome P450s, for selective C-H oxidation reactions

(2017) H. Christine Lo et al.   JOURNAL OF ORGANOMETALLIC CHEMISTRY

Selection of an endogenous 2,3-butanediol pathway in Escherichia coli by fermentative redox balance

(2017) Keming Liang et al.   METABOLIC ENGINEERING

Engineering the cofactor specificity of an alcohol dehydrogenase via single mutations or insertions distal to the 2′-phosphate group of NADP(H)

(2017) Kusum Solanki et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer

(2017) Lei Wang et al.   ACS Catalysis

Enzymatic Reduction of Nicotinamide Biomimetic Cofactors Using an Engineered Glucose Dehydrogenase: Providing a Regeneration System for Artificial Cofactors

(2017) Claudia Nowak et al.   ACS Catalysis

P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations

(2016) Nina Beyer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Switch in Cofactor Specificity of a Baeyer-Villiger Monooxygenase

(2016) Andy Beier et al.   CHEMBIOCHEM

Better than Nature: Nicotinamide Biomimetics That Outperform Natural Coenzymes

(2016) Tanja Knaus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nonenzymatic Regeneration of Styrene Monooxygenase for Catalysis

(2015) Caroline E. Paul et al.   ACS Catalysis

Tuning Enzyme Kinetics through Designed Intermolecular Interactions Far from the Active Site

(2015) Yingning Gao et al.   ACS Catalysis

A water-forming NADH oxidase from Lactobacillus pentosus suitable for the regeneration of synthetic biomimetic cofactors

(2015) Claudia Nowak et al.   Frontiers in Microbiology

SoxR as a Single-Cell Biosensor for NADPH-Consuming Enzymes in Escherichia coli

(2013) Solvej Siedler et al.   ACS Synthetic Biology

Mimicking Nature: Synthetic Nicotinamide Cofactors for C═C Bioreduction Using Enoate Reductases

(2012) Caroline E. Paul et al.   ORGANIC LETTERS

Enzymatic biofuel cells utilizing a biomimetic cofactor

(2011) Elliot Campbell et al.   CHEMICAL COMMUNICATIONS

Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide

(2011) Debin Ji et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering Cytochrome P450 Enzymes for Improved Activity towards Biomimetic 1,4-NADH Cofactors

(2008) Jessica D. Ryan et al.   CHEMBIOCHEM