Primary Hepatocyte Isolation and Cultures: Technical Aspects, Challenges and Advancements

Mimicking Native Liver Lobule Microarchitecture In Vitro with Parenchymal and Non-parenchymal Cells Using 3D Bioprinting for Drug Toxicity and Drug Screening Applications

(2022) G. Janani et al.   ACS Applied Materials & Interfaces

Generation of proliferating human adult hepatocytes using optimized 3D culture conditions

(2021) Sophie Rose et al.   Scientific Reports

Investigation and comparison of new galactosylation methods on PCL/chitosan scaffolds for enhanced liver tissue engineering

(2021) Fatemeh Ghahremanzadeh et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

3D bioprinting of hepatocytes: core–shell structured co-cultures with fibroblasts for enhanced functionality

(2021) Rania Taymour et al.   Scientific Reports

Peptide-based scaffolds for the culture and maintenance of primary human hepatocytes

(2021) Douglas MacPherson et al.   Scientific Reports

Hepatocyte organoids and cell transplantation: What the future holds

(2021) Weng Chuan Peng et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Optimized 3D Culture of Hepatic Cells for Liver Organoid Metabolic Assays

(2021) Christian Moya Gamboa et al.   Cells

Microtechnology-based in vitro models: Mimicking liver function and pathophysiology

(2021) Seung Yeon Lee et al.   APL Bioengineering

Functionality of primary hepatic non-parenchymal cells in a 3D spheroid model and contribution to acetaminophen hepatotoxicity

(2020) Catherine C. Bell et al.   ARCHIVES OF TOXICOLOGY

Pluripotent-Stem-Cell-Derived Hepatic Cells: Hepatocytes and Organoids for Liver Therapy and Regeneration

(2020) Antonietta Messina et al.   Cells

A 3D Cell Culture Organ-on-a-Chip Platform With a Breathable Hemoglobin Analogue Augments and Extends Primary Human Hepatocyte Functions in vitro

(2020) James T. Shoemaker et al.   Frontiers in Molecular Biosciences

A New Panel-Based Next-Generation Sequencing Method for ADME Genes Reveals Novel Associations of Common and Rare Variants With Expression in a Human Liver Cohort

(2019) Kathrin Klein et al.   Frontiers in Genetics

A cell lines derived microfluidic liver model for investigation of hepatotoxicity induced by drug-drug interaction

(2019) Jiu Deng et al.   Biomicrofluidics

Implantable Vascularized Liver Chip for Cross‐Validation of Disease Treatment with Animal Model

(2019) Jung Bok Lee et al.   ADVANCED FUNCTIONAL MATERIALS

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

(2019) Giuseppe Pettinato et al.   Scientific Reports

Reproducing human and cross-species drug toxicities using a Liver-Chip

(2019) Kyung-Jin Jang et al.   Science Translational Medicine

Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis

(2019) Ross Dobie et al.   Cell Reports

Comparison of Drug Metabolism and Its Related Hepatotoxic Effects in HepaRG, Cryopreserved Human Hepatocytes, and HepG2 Cell Cultures

(2018) Yuichi Yokoyama et al.   BIOLOGICAL & PHARMACEUTICAL BULLETIN

Comparison of Hepatic 2D Sandwich Cultures and 3D Spheroids for Long-term Toxicity Applications: A Multicenter Study

(2018) Catherine C Bell et al.   TOXICOLOGICAL SCIENCES

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

(2018) Huili Hu et al.   CELL

Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture

(2018) Weng Chuan Peng et al.   CELL

In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity

(2018) Kun Zhang et al.   Cell Stem Cell

Long-term liver-specific functions of hepatocytes in electrospun chitosan nanofiber scaffolds coated with fibronectin

(2017) Divya Rajendran et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Hepatoprotective effects of ethanol extracts from Folium Syringae against acetaminophen-induced hepatotoxicity in vitro and in vivo

(2017) Chen-Xi Shi et al.   Journal of the Chinese Medical Association

Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip

(2017) Yu Du et al.   LAB ON A CHIP

Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease

(2017) Tomasz Kostrzewski et al.   WORLD JOURNAL OF GASTROENTEROLOGY

Immunofluorescence identifies distinct subsets of endothelial cells in the human liver

(2017) Otto Strauss et al.   Scientific Reports

Bile canaliculi formation and biliary transport in 3D sandwich-cultured hepatocytes in dependence of the extracellular matrix composition

(2016) Daniela Deharde et al.   ARCHIVES OF TOXICOLOGY

Fluid shear stress modulation of hepatocyte-like cell function

(2016) Hassan Rashidi et al.   ARCHIVES OF TOXICOLOGY

A 3D alcoholic liver disease model on a chip

(2016) JaeSeo Lee et al.   Integrative Biology

Engineering a perfusable 3D human liver platform from iPS cells

(2016) Arnout Schepers et al.   LAB ON A CHIP

Monocyte-induced recovery of inflammation-associated hepatocellular dysfunction in a biochip-based human liver model

(2016) Marko Gröger et al.   Scientific Reports

A Microfabricated Platform for Generating Physiologically-Relevant Hepatocyte Zonation

(2016) William J. McCarty et al.   Scientific Reports

Isolation and co-culture of rat parenchymal and non-parenchymal liver cells to evaluate cellular interactions and response

(2016) Shyam Sundhar Bale et al.   Scientific Reports

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Long-term maintenance of a microfluidic 3D human liver sinusoid

(2015) Ljupcho Prodanov et al.   BIOTECHNOLOGY AND BIOENGINEERING

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Heparin hydrogel sandwich cultures of primary hepatocytes

(2015) Elena Foster et al.   EUROPEAN POLYMER JOURNAL

Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow

(2015) Mandy B. Esch et al.   LAB ON A CHIP

HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism

(2014) Sofia P. Rebelo et al.   ARCHIVES OF TOXICOLOGY

Rapid and Sensitive Assessment of Human Hepatocyte Functions

(2014) Roberto Gramignoli et al.   CELL TRANSPLANTATION

Galactosylated electrospun membranes for hepatocyte sandwich culture

(2014) Hsiu-Wen Chien et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Molecular mechanisms underlying the enhanced functions of three-dimensional hepatocyte aggregates

(2013) Tammy T. Chang et al.   BIOMATERIALS

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins

(2012) H. H. J. Gerets et al.   CELL BIOLOGY AND TOXICOLOGY

Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model

(2011) Sau Fung Wong et al.   BIOMATERIALS

Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes

(2011) Bruna Vinci et al.   Biotechnology Journal

In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor

(2011) Daniel Mueller et al.   Journal of Tissue Engineering and Regenerative Medicine

HepaRG cells: A human model to study mechanisms of acetaminophen hepatotoxicity

(2010) Mitchell R. McGill et al.   HEPATOLOGY

Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants

(2010) Vasiliy N. Goral et al.   LAB ON A CHIP

A Comparative Study of Genome-Wide Transcriptional Profiles of Primary Hepatocytes in Collagen Sandwich and Monolayer Cultures

(2010) Yeonhee Kim et al.   TISSUE ENGINEERING PART C-METHODS

Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype

(2009) Yuanyuan Zhang et al.   BIOMATERIALS

Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells

(2009) Petra Krause et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage

(2009) Gabriella Brolén et al.   JOURNAL OF BIOTECHNOLOGY

Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture

(2009) Jinyong Fan et al.   JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

A microfluidic 3D hepatocyte chip for drug toxicity testing

(2009) Yi-Chin Toh et al.   LAB ON A CHIP

A new technique for primary hepatocyte expansion in vitro

(2008) Cheul H. Cho et al.   BIOTECHNOLOGY AND BIOENGINEERING