Internal reference genes with the potential for normalizing quantitative PCR results for oral fluid specimens

Consistent Detection of 2019 Novel Coronavirus in Saliva

(2020) Kelvin Kai-Wang To et al.   CLINICAL INFECTIOUS DISEASES

Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay

(2020) Angela Nagel et al.   PLoS One

Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study

(2020) E. Pasomsub et al.   CLINICAL MICROBIOLOGY AND INFECTION

Saliva is a reliable tool to detect SARS-CoV-2

(2020) Lorenzo Azzi et al.   JOURNAL OF INFECTION

Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA

(2020) Ting-Yu Cheng et al.   JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-analytics

(2020) Daniela Basso et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Clinical evaluation of self-collected saliva by RT-qPCR, direct RT-qPCR, RT-LAMP, and a rapid antigen test to diagnose COVID-19

(2020) Mayu Nagura-Ikeda et al.   JOURNAL OF CLINICAL MICROBIOLOGY

SARS-CoV-2 detection, viral load and infectivity over the course of an infection

(2020) Kieran A. Walsh et al.   JOURNAL OF INFECTION

Ct values from SARS-CoV-2 diagnostic PCR assays should not be used as direct estimates of viral load

(2020) Elias Dahdouh et al.   JOURNAL OF INFECTION

Guidelines for oral fluid-based surveillance of viral pathogens in swine

(2020) Alexandra Henao-Diaz et al.   Porcine Health Management

Estimating time since deposition using quantification of RNA degradation in body fluid-specific markers

(2019) Suaad Alshehhi et al.   FORENSIC SCIENCE INTERNATIONAL

Inhibition monitoring in veterinary molecular testing

(2019) Lifang Yan et al.   JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

Structural and evolutionary insights into ribosomal RNA methylation

(2018) Petr V Sergiev et al.   Nature Chemical Biology

The life of U6 small nuclear RNA, from cradle to grave

(2018) Allison L. Didychuk et al.   RNA

Suitability of group-level oral fluid sampling in ruminant populations for lumpy skin disease virus detection

(2018) K. Dietze et al.   VETERINARY MICROBIOLOGY

U6 can be used as a housekeeping gene for urinary sediment miRNA studies of IgA nephropathy

(2018) Zhi-Yu Duan et al.   Scientific Reports

Development of a universal internal positive control

(2018) Mark F Kavlick   BIOTECHNIQUES

Effects of sample handling on the detection of porcine reproductive and respiratory syndrome virus in oral fluids by reverse-transcription real-time PCR

(2018) Ashley C. Weiser et al.   JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

Clinical and Molecular Features of Feline Foamy Virus and Feline Leukemia Virus Co-Infection in Naturally-Infected Cats

(2018) Liliane Cavalcante et al.   Viruses-Basel

High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

(2017) Grant C O’Connell et al.   Labmedicine

Selection of internal reference genes for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in the rumen epithelium

(2017) Jose V. Die et al.   PLoS One

Direct detection and characterization of foot-and-mouth disease virus in East Africa using a field-ready real-time PCR platform

(2017) E. L. A. Howson et al.   Transboundary and Emerging Diseases

Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation

(2017) Michela Masè et al.   Scientific Reports

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

(2017) Petr Kralik et al.   Frontiers in Microbiology

High Interspecimen Variability in Nucleic Acid Extraction Efficiency Necessitates the Use of Spike-In Control for Accurate qPCR-based Measurement of Plasma Cell-Free DNA Levels

(2017) Grant C O’Connell et al.   LABORATORY MEDICINE

Validation of Reference Genes for Quantitative Real-Time PCR in Bovine PBMCs Transformed and Non-transformed by Theileria annulata

(2016) Hongxi Zhao et al.   KOREAN JOURNAL OF PARASITOLOGY

Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil

(2016) Myrna C. Bonaldo et al.   PLoS Neglected Tropical Diseases

Validation of Reference Genes for Oral Cancer Detection Panels in a Prospective Blinded Cohort

(2016) Jack L. Martin   PLoS One

Evaluation of housekeeping genes for normalizing real-time quantitative PCR assays in pig skeletal muscle at multiple developmental stages

(2015) Yueying Wang et al.   GENE

Differential distribution of U6 (RNU6-1) expression in human carcinoma tissues demonstrates the requirement for caution in the internal control gene selection for microRNA quantification

(2015) GE LOU et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

A new system for naming ribosomal proteins

(2014) Nenad Ban et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

What can go wrong at the data normalization step for identification of biomarkers?

(2014) P. Filzmoser et al.   JOURNAL OF CHROMATOGRAPHY A

Validation of reference genes in the feline endometrium

(2014) Ewelina Jursza et al.   Reproductive Biology

Saliva and Plasma Quantitative Polymerase Chain Reaction–Based Detection and Surveillance of Human Papillomavirus–Related Head and Neck Cancer

(2014) Sun M. Ahn et al.   JAMA Otolaryngology-Head & Neck Surgery

Validation of housekeeping genes for studying differential gene expression in the bovine myometrium

(2013) Robert Rekawiecki et al.   ACTA VETERINARIA HUNGARICA

Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues

(2013) Emanuela de Lima Rebouças et al.   BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

Reference genes in real-time PCR

(2013) Bartłomiej Kozera et al.   JOURNAL OF APPLIED GENETICS

Degradation of MUC7 and MUC5B in Human Saliva

(2013) Sachiko Takehara et al.   PLoS One

Identification and Validation of Reference Genes for qPCR Detection of Serum microRNAs in Colorectal Adenocarcinoma Patients

(2013) Guixi Zheng et al.   PLoS One

Validation of a commercial real-time PCR kit for specific and sensitive detection of Pseudorabies

(2012) Françoise Pol et al.   JOURNAL OF VIROLOGICAL METHODS

Neuropeptide Y2 Receptor (NPY2R) Expression in Saliva Predicts Feeding Immaturity in the Premature Neonate

(2012) Jill L. Maron et al.   PLoS One

GAPDH: A common enzyme with uncommon functions

(2011) Craig Nicholls et al.   CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY

Detection of Influenza a Virus in Porcine Oral Fluid Samples

(2011) Susan E. Detmer et al.   JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION

β-Actin specifically controls cell growth, migration, and the G-actin pool

(2011) Tina M. Bunnell et al.   MOLECULAR BIOLOGY OF THE CELL

Efficient surveillance of pig populations using oral fluids

(2011) Alejandro Ramirez et al.   PREVENTIVE VETERINARY MEDICINE

Identification of suitable reference genes for measurement of gene expression in human cervical tissues

(2010) Yuanming Shen et al.   ANALYTICAL BIOCHEMISTRY

Selection of optimal reference genes for normalization in quantitative RT-PCR

(2010) Inna Chervoneva et al.   BMC BIOINFORMATICS

The diverse functions of GAPDH: Views from different subcellular compartments

(2010) Carlos Tristan et al.   CELLULAR SIGNALLING

Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue

(2010) Antje Koppelkamm et al.   INTERNATIONAL JOURNAL OF LEGAL MEDICINE

mRNA and microRNA quality control for RT-qPCR analysis

(2010) C. Becker et al.   METHODS

Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes

(2010) Veronica Spalenza et al.   VETERINARY JOURNAL

Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues

(2009) Yvonne Kessler et al.   BMC MOLECULAR BIOLOGY

Selection of reliable reference genes for gene expression studies in peach using real-time PCR

(2009) Zhaoguo Tong et al.   BMC MOLECULAR BIOLOGY

Pre-mRNA Processing Reaches Back toTranscription and Ahead to Translation

(2009) Melissa J. Moore et al.   CELL

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references

(2009) S. Guenin et al.   JOURNAL OF EXPERIMENTAL BOTANY

Selection and Validation of Candidate Housekeeping Genes for Studies of Human Keratinocytes—Review and Recommendations

(2009) Michael Bär et al.   JOURNAL OF INVESTIGATIVE DERMATOLOGY

A novel and universal method for microRNA RT-qPCR data normalization

(2009) Pieter Mestdagh et al.   GENOME BIOLOGY

Effects of dimethyl sulfoxide and dexamethasone on mRNA expression of housekeeping genes in cultures of C2C12 myotubes

(2008) Masuhiro Nishimura et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Mucins in the mucosal barrier to infection

(2008) S K Linden et al.   Mucosal Immunology

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device

(2008) Simant Dube et al.   PLoS One

Normalization of microRNA expression levels in quantitative RT-PCR assays: Identification of suitable reference RNA targets in normal and cancerous human solid tissues

(2008) H. J. Peltier et al.   RNA

Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues

(2008) Kathleen A. McIntosh et al.   VETERINARY MICROBIOLOGY