Article
Food Science & Technology
Likun Long, Zhenjuan Xing, Yuxuan He, Wei Yan, Congcong Li, Wei Xia, Liming Dong, Ning Zhao, Yue Ma, Yanbo Xie, Na Liu, Feiwu Li
Summary: This study investigated the suitability of using endogenous reference gene (ERG) characteristics for the quantification of genetically modified (GM) canola events using digital PCR (dPCR). It found that BnAcc and BnC1 were more specific and stable in copy number compared to other genes. Intra-laboratory validation showed that the ddPCR methods for BnAcc and BnC1 were feasible for the quantification of GM canola. These methods can provide technical support for GM labeling regulations.
Article
Multidisciplinary Sciences
T. Toorani, P. M. Mackie, G. F. Mastromonaco
Summary: Proper normalization of RT-qPCR data is crucial for interpreting results and ensuring the accuracy of scientific conclusions. In untreated bovine fibroblasts, a combined use of GUSB and RPL13A was determined to be the best approach for data normalization.
SCIENTIFIC REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Na Wang, Yulan Lin, Liling Shen, Haoyuan Song, Weihai Huang, Jingyao Huang, Fa Chen, Fengqiong Liu, Jing Wang, Yu Qiu, Bin Shi, Lisong Lin, Baochang He
Summary: A large-scale prospective cohort study was conducted to assess the prognostic role of pretreatment lymphocyte percentage (LY%) in patients with oral squamous cell carcinoma (OSCC). The study found that a high pretreatment LY% was significantly associated with better disease-specific survival of patients with OSCC.
Article
Genetics & Heredity
Xiaoxia Wen, Guishu Yang, Yongcheng Dong, Liping Luo, Bangrong Cao, Birga Anteneh Mengesha, Ruiling Zu, Yulin Liao, Chang Liu, Shi Li, Yao Deng, Kaijiong Zhang, Xin Ma, Jian Huang, Dongsheng Wang, Keyan Zhao, Ping Leng, Huaichao Luo
Summary: Many studies have shown that certain messenger RNA (mRNA) in platelets can serve as biomarkers for diagnosing pan-cancer. This study used bioinformatics and functional analysis to identify and validate reference genes for pan-cancer diagnosis based on platelet transcript analysis. The results confirmed that GAPDH gene was the most suitable reference gene for this purpose, providing a platform for molecular diagnosis of pan-cancer using platelets.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Tung-Shing Mamie Lih, Mingming Dong, Leslie Mangold, Alan Partin, Hui Zhang
Summary: This study investigated the clinical utility of urinary marker panels composed of urinary glycopeptides and/or urinary prostate-specific antigen (PSA) for distinguishing non-aggressive from aggressive prostate cancer. The multi-marker panels showed clinically meaningful results with area under the curve (AUC) ranging from 0.70 to 0.85.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Qiang Song, Lu Dou, Wenjin Zhang, Yang Peng, Man Huang, Mengyuan Wang
Summary: Novel and reliable RG combinations for breast cancer research were identified using a public RNA-seq dataset, providing a solid foundation for accurate normalization of qRT-PCR results across different breast cancer tissues and cells.
BIOMEDICAL ENGINEERING ONLINE
(2021)
Article
Biotechnology & Applied Microbiology
Yao Dai, Yumeng Cao, Jens Kohler, Aiping Lu, Shaohua Xu, Haiyun Wang
Summary: This study identified OAZ1 and hsa-miR-6835-3p as the most reliable individual reference genes for mRNA and miRNA quantification in cancer exosomes. A combination of reference genes - OAZ1/SERF2/MPP1 for mRNA and hsa-miR-6835-3p/hsa-miR-4468-3p for miRNA analyses is recommended for superior accuracy.
Article
Genetics & Heredity
Xin Yang, Mikael Eriksson, Kamila Czene, Andrew Lee, Goska Leslie, Michael Lush, Jean Wang, Joe Dennis, Leila Dorling, Sara Carvalho, Nasim Mavaddat, Jacques Simard, Marjanka K. Schmidt, Douglas F. Easton, Per Hall, Antonis C. Antoniou
Summary: The BOADICEA model provides valid breast cancer risk predictions, with the polygenic risk score contributing most to risk stratification. The full multifactorial model shows good calibration and discrimination when considering all risk factors or subsets of risk factors in risk prediction.
JOURNAL OF MEDICAL GENETICS
(2022)
Article
Multidisciplinary Sciences
Yang Hu, Qi Jiang, Xiang Zhai, Liang Liu, Yuntian Hong
Summary: This study screened and verified the optimal panel of reference genes in colorectal cancer and normal colonic cell lines. The results showed that the expression levels of candidate reference genes varied in different cell lines, but the optimal panel of reference genes always consisted of two genes. Moreover, the optimal combination of reference genes varied among different cell lines.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Anna Maria Wirsing, Inger-Heidi Bjerkli, Sonja Eriksson Steigen, Oddveig Rikardsen, Synnove Norvoll Magnussen, Beate Hegge, Marit Seppola, Lars Uhlin-Hansen, Elin Hadler-Olsen
Summary: This study validated the prognostic value of selected markers for head and neck cancer in oral tongue squamous cell carcinoma, highlighting the importance of subsite-specific analyses for accurate prognosis.
Article
Nutrition & Dietetics
Halla Kaminska, Pawel Wieczorek, Grzegorz Zalewski, Beata Malachowska, Przemyslaw Kucharski, Wojciech Fendler, Lukasz Szarpak, Przemyslawa Jarosz-Chobot
Summary: We defined reference ranges of glycemic variability indices for non-diabetic infants during post-operative intensive care treatment using continuous glucose monitoring data. We found a high likelihood of hyperglycemia on the first postoperative day and recommend the widespread use of glucose monitoring systems in pediatric intensive care units.
Article
Oncology
Anouk E. Hentschel, Irene J. Beijert, Judith Bosschieter, Paul C. Kauer, Andre N. Vis, Birgit Lissenberg-Witte, R. Jeroen A. van Moorselaar, Renske D. M. Steenbergen, Jakko A. Nieuwenhuijzen
Summary: This study validated the feasibility of using DNA methylation analysis in urine for non-invasive detection of bladder cancer, and confirmed the diagnostic potential of the GHSR/MAL marker panel.
CLINICAL EPIGENETICS
(2022)
Article
Multidisciplinary Sciences
Mary Wallace, Lynne K. Rieske
Summary: The study found that in the six-spined ips, Ips calligraphus, the 16s and 28s genes are the most stable and suitable reference genes for gene expression studies, especially in evaluating RNAi.
SCIENTIFIC REPORTS
(2021)
Article
Health Care Sciences & Services
Huei-Tzu Chien, Chi-Chin Yeh, Chi-Kuang Young, Tzu-Ping Chen, Chun-Ta Liao, Hung-Ming Wang, Kai-Lun Cho, Shiang-Fu Huang
Summary: Head and neck cancer patients who are habitual drinkers and carriers of certain genetic factors are at higher risk of developing multiple primary tumors. Establishing a polygenic scoring model can effectively predict the risk of multiple primary cancers. Routine screening of the upper digestive tract is recommended for patients with high scores in the polygenic model to detect new primary tumors early.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Genetics & Heredity
Guangyan Li, Xiaohui Sun, Xiaoping Zhu, Bin Wu, Hao Hong, Zhimei Xin, Xiangqi Xin, Jiejun Peng, Shanshan Jiang
Summary: Quantitative real-time PCR (qRT-PCR) in sweet potatoes requires accurate data normalization, but there is a lack of studies on appropriate reference genes for gene expression analysis. This study examined the expression variations of eight candidate reference genes in the leaf and root tissues of sweet potatoes. The results showed that different viral infections and origin tissues affected the expression levels of these genes. The research provides appropriate reference genes for further analysis in leaf and root samples of viruses in sweet potatoes.