Journal
REPRODUCTIVE BIOLOGY
Volume 14, Issue 4, Pages 302-306Publisher
INST ANIMAL REPRODUCTION FOOD RESEARCH
DOI: 10.1016/j.repbio.2014.04.003
Keywords
Reference gene; Endometrium; Domestic cat
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Funding
- Polish Ministry of Scientific Research and High Education [N308 560740]
- European Union
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The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM). According to NormFinder and BestKeeper, ACTB (at all examined primer concentrations) followed by RPS7 and CYC were the most stable genes. Based on geNorm results at least two genes from among RPS7, RPL30, ACTB or YWHAZ should be chosen for Real Time-PCR result normalization. (C) 2014 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
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