Article
Multidisciplinary Sciences
Guo-Hua Wang, Cheng-Cheng Liang, Bing-Zhi Li, Xin-Ze Du, Wen-Zhen Zhang, Gong Cheng, Lin-Sen Zan
Summary: This study assessed the accuracy of sixteen commonly used internal reference genes in skeletal muscle-derived satellite cells of Qinchuan cattle at different stages of proliferation and induction of differentiation, and determined the most suitable gene combinations. The results provide a foundation for selecting reference genes for qRT-PCR during the proliferation and induction of differentiation of bovine skeletal muscle-derived satellite cells.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
T. Toorani, P. M. Mackie, G. F. Mastromonaco
Summary: Proper normalization of RT-qPCR data is crucial for interpreting results and ensuring the accuracy of scientific conclusions. In untreated bovine fibroblasts, a combined use of GUSB and RPL13A was determined to be the best approach for data normalization.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Guo-Hua Wang, Si-Hu Wang, Wen-Zhen Zhang, Cheng-Cheng Liang, Gong Cheng, Xiao-Yu Wang, Yu Zhang, Lin-Sen Zan
Summary: The stability of internal reference genes is crucial for accurate gene expression analysis. This study identified stable reference genes for analyzing the expression of proliferation-related and differentiation-inducing genes in bovine primary preadipocytes using qRT-PCR. Multiple algorithms were used to evaluate the stability of 16 candidate reference genes, and the results identified the most stable and least stable genes in the proliferation and differentiation stages of these cells. This study provides a foundation for further research on the mechanism of proliferation and differentiation of bovine primary preadipocytes using qRT-PCR.
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Pauline Chatelain, Cecile Blanchard, Jeremy Astier, Agnes Klinguer, David Wendehenne, Sylvain Jeandroz, Claire Rosnoblet
Summary: Microalgae, especially the green alga Klebsormidium nitens, have recently gained attention as important biological models, particularly for studying the transition stages leading to terrestrial life colonization. This study identified optimal reference gene pairs for normalizing RT-qPCR data in K. nitens in response to abiotic stresses. The identified genes also served as marker genes in response to the three tested stresses.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Horticulture
Tangjie Nie, Zheng Jiang, Liyong Sun, Yao Chen, Jia Li, Aixiang Yang, Qiang Wei, Zengfang Yin
Summary: The present study screened suitable reference genes based on transcriptome data and evaluated their expression stability using four statistical algorithms in different flowering transition events. GBP, UBQ, UBC, and GAPDH were identified as the most stable genes in floral transition, summer flowering initiation, spring flowering initiation, and dormancy, respectively. qPCR results using UBC+GBP as the reference genes showed the highest correlation with high throughput sequencing data. These findings suggest that reference gene selection is developmental stage-dependent and provide technical support for molecular regulation studies in magnolias and other species.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Meng Wang, Zhouwen Wang, Shuangshuang Wei, Jun Xie, Jiaquan Huang, Dongdong Li, Wenbin Hu, Hongli Li, Hua Tang
Summary: In this study, we evaluated the suitability of eight candidate reference genes for normalizing gene expression levels estimated by RT-qPCR in N. dimidiatum. Tubulin and Actin (1) were identified as the most stably expressed reference genes under different temperatures, while Actin (1) and Actin were the most stably expressed reference genes at different developmental stages. Tubulin and UBQ were the most stably expressed reference genes during interaction with pitaya. Actin and 18s rRNA were the most stably expressed across all experimental conditions. Our results provide insights that will aid future RT-qPCR studies of gene expression in N. dimidiatum.
SCIENTIFIC REPORTS
(2022)
Review
Plant Sciences
Umair Ahmed, Qi Xie, Xueping Shi, Bo Zheng
Summary: This article reviews the importance of developing appropriate reference genes (RGs) for accurately quantifying gene expression levels, and discusses the strategies and processes for developing RGs using different techniques and methods. The most commonly used RGs in horticultural plants and their development strategies are also discussed in detail. The information provided in this article will help in designing effective strategies for developing RGs in horticultural plants.
CRITICAL REVIEWS IN PLANT SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xiaoxing Yang, Guangxiang Tong, Le Dong, Ting Yan, Huan Xu, Guopan Tang, Yongquan Zhang, Kai Ma, Jiasheng Yin, Youyi Kuang
Summary: This study evaluated the stability of 10 commonly used candidate reference genes in taimen and identified RPS29 and RPL19 as the optimal reference genes for qRT-PCR analysis of gene expression in taimen.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Junming Zhao, Jian Yang, Xiaoyun Wang, Yanli Xiong, Yi Xiong, Zhixiao Dong, Xiong Lei, Lijun Yan, Xiao Ma
Summary: This study evaluated the expression stability of 14 candidate reference genes in K. melanthera under different treatments and identified the most stable and suitable reference genes under stressed conditions. The results provide beneficial information for the selection of stable and reliable reference genes for qRT-PCR in K. melanthera.
Article
Multidisciplinary Sciences
Magdalena Sozoniuk, Monika Jamiol, Marta Kankofer, Krzysztof Kowalczyk
Summary: This study aimed to identify endogenous reference genes with stable expression for transcriptional regulation research during pregnancy establishment and maintenance. RPS9 and SUZ12 were found to be the most suitable reference genes, and they can be used for studying gene expression in both normal and compromised pregnancies.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jing-Rong Zhang, Yuan-Yuan Feng, Ma-Jin Yang, Yu Xiao, Yu-Shan Liu, Yuan Yuan, Zhen Li, Yan Zhang, Ming Zhuo, Jun Zhang, Cai-Xia Li
Summary: This study selected and evaluated 11 reference genes in okra, and found that eIF4A and PP2A were the most stable ones. Using these two stable reference genes can normalize gene expression in okra, facilitating further functional gene research.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Sunil Kumar, Ayaan Ahmad, Namrata Kushwaha, Niti Shokeen, Sheetal Negi, Kamini Gautam, Anup Singh, Pavan Tiwari, Rakesh Garg, Richa Agarwal, Anant Mohan, Anjan Trikha, Alok Thakar, Vikram Saini
Summary: This study investigated the expression stability of nine commonly used reference genes in patients with COVID-19 and COVID-19-associated mucormycosis. The results showed that CypA is the most suitable reference gene, while GAPDH is the least suitable. The expression stability of reference genes is influenced by disease severity.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Fulvio Riondato, Valeria Martini, Maverick Melega, Alessia Poggi, Barbara Miniscalco
Summary: The study described proliferative and apoptotic rates in different canine lymphoma subtypes at diagnosis, revealing variations in proliferation and apoptosis levels among different types of lymphoma, providing groundwork for future clinical and pharmacological studies.
RESEARCH IN VETERINARY SCIENCE
(2021)
Review
Veterinary Sciences
Fulvio Riondato, Stefano Comazzi
Summary: This review discusses the use of flow cytometry to improve the diagnosis of canine B cell lymphoma, with a focus on identifying specific immunotypes, potential prognostic markers, staging, and minimal residual disease.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Valeria Martini, Giuseppe Marano, Luca Aresu, Ugo Bonfanti, Patrizia Boracchi, Mario Caniatti, Francesco Cian, Matteo Gambini, Laura Marconato, Carlo Masserdotti, Arturo Nicoletti, Fulvio Riondato, Paola Roccabianca, Damiano Stefanello, Erik Teske, Stefano Comazzi
Summary: Cytopathology is a valuable tool for diagnosing lymphoma in dogs, with high accuracy for overall lymphoma diagnosis but lower accuracy for subtype characterization. It can be used as a screening test to predict grade and phenotype, but confirmation with additional techniques like flow cytometry and immunohistochemistry is necessary. Interobserver agreement for identifying lymphoma cases and subtype characterization was moderate.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Valentina Bongiorno, Achille Schiavone, Manuela Renna, Stefano Sartore, Dominga Soglia, Paola Sacchi, Marta Gariglio, Annelisse Castillo, Cecilia Mugnai, Claudio Forte, Chiara Bianchi, Silvia Mioletti, Laura Gasco, Ilaria Biasato, Alberto Brugiapaglia, Federico Sirri, Marco Zampiga, Francesco Gai, Margherita Marzoni, Silvia Cerolini, Sihem Dabbou
Summary: This research investigated the slaughter performance and meat quality of two native Italian chicken breeds, Bionda Piemontese and Bianca di Saluzzo. The results showed that slaughtering at 7 months of age was associated with the best slaughter performance and meat quality characteristics in both breeds.
Article
Veterinary Sciences
Sara Damiano, Watanya Jarriyawattanachaikul, Flavia Girolami, Consiglia Longobardi, Carlo Nebbia, Emanuela Andretta, Chiara Lauritano, Sihem Dabbou, Giuseppina Avantaggiato, Achille Schiavone, Paola Badino, Roberto Ciarcia
Summary: Aflatoxin B1 (AFB1) is a human carcinogen that causes hepatotoxicity, immunotoxicity, and kidney damage. Chickens are moderately sensitive to AFB1, leading to reduced growth performance and economic losses. The antioxidant activity of Curcumin (CURC) can help prevent oxidative stress triggered by AFB1 and protect kidney tissue.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Michela Cantiello, Monica Carletti, Mery Giantin, Giulia Gardini, Francesca Capolongo, Paolo Cascio, Marianna Pauletto, Flavia Girolami, Mauro Dacasto, Carlo Nebbia
Summary: This study provides a comprehensive characterization of phenobarbital's (PB) effects on drug-metabolizing enzyme (DME) catalytic activities in cattle liver. The findings show that PB increases the protein amounts and enzyme activities of certain target enzymes, but has contradictory effects on others. The study also reveals an increase in 26S proteasome activity, suggesting a potential role in cattle DME regulation. The results further confirm species differences in DME expression and activity between cattle, humans, and rodents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
G. Cagnotti, C. Corona, F. Riondato, B. Iulini, M. D. Pintore, A. D'Angelo
Summary: This is a rare case of acute myelomonocytic leukemia subtype M4 with spinal cord infiltration in a dog, which was euthanized due to clinical deterioration. It is important to consider myelomonocytic leukemia in the differential diagnosis of patients with neurological signs due to spinal cord localization.
SCHWEIZER ARCHIV FUR TIERHEILKUNDE
(2022)
Article
Food Science & Technology
Flavia Girolami, Andrea Barbarossa, Paola Badino, Shiva Ghadiri, Damiano Cavallini, Anna Zaghini, Carlo Nebbia
Summary: This study investigated the effect of turmeric powder on the mammary excretion of aflatoxin M1 (AFM1) in cows. The results showed that turmeric powder did not significantly reduce AFM1 content in milk.
Article
Biochemical Research Methods
Fulvio Riondato, Barbara Colitti, Sergio Rosati, Federica Sini, Valeria Martini
Summary: This study introduces a method for quickly screening antibodies, addressing the issue of cross-reacting antibodies and antibodies for flow cytometry in the veterinary field. This method eliminates the need to search for positive biological samples and serves as the initial step for selecting the best antibodies.
Article
Agriculture, Dairy & Animal Science
Riccardo Moretti, Stefania Chessa, Stefano Sartore, Dominga Soglia, Daniele Giaccone, Francesca Tiziana Cannizzo, Paola Sacchi
Summary: This study examined the application of genomic tools in animal selection on farms and found that well-managed farms rear animals with better genomic indexes. Selecting heifers based on their wellness genomic indexes can improve their economic value and disease resistance. Genomic selection is a fast and cost-effective method for animal evaluation, although it is mainly used for sire line and performance traits.
Article
Agriculture, Dairy & Animal Science
Alessia Candellone, Paola Badino, Flavia Girolami, Ugo Ala, Floriana Mina, Rosangela Odore
Summary: Antimicrobial resistance (AMR) is a global health concern that requires the One Health approach to manage. Pet-owner collaboration with prescribing vets is crucial for the effective use of antibiotics in companion animals. An online survey of dog owners revealed their awareness of AMR and their willingness to use alternatives to antibiotics for the management of canine acute diarrhea. Further efforts are needed to increase public knowledge on AMR and to develop treatment guidelines for uncomplicated canine AD.
Article
Veterinary Sciences
Alessia Candellone, Paola Badino, Flavia Girolami, Matteo Cerquetella, Patrizia Nebbia, Luca Aresu, Simona Zoppi, Domenico Bergero, Rosangela Odore
Summary: The use of unbalanced, poultry-based raw diets for dogs has increased in recent years, leading to potential risks of bacterial infections for both pets and humans. This article presents a case of Campylobacter infection in a puppy and its owner, highlighting the importance of a collaborative approach among veterinarians, physicians, and caregivers to prevent the spread of zoonotic diseases.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Fulvio Riondato, Alessia Poggi, Barbara Miniscalco, Federica Sini, Laura Marconato, Valeria Martini
Summary: In-depth understanding of non-neoplastic patterns is crucial for neoplasia diagnosis. This study examined the flow cytometric features of B- and T-lymphocytes in reactive lymph nodes and lymphomas, as well as reported proliferative activity. Reactive lymph nodes were composed of a mixed population of small and large T and B cells, with differences in size and CD5 expression. Large B-cells exhibited larger size and higher CD5 expression in lymphomas compared to reactive lymph nodes. The study also identified a subset of small CD5+CD21+dim cells in reactive lymph nodes. Ki-67% values were elevated in both reactive lymph nodes and lymphomas.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Riccardo Moretti, Stefano Sartore, Barbara Colitti, Margherita Profiti, Stefania Chessa, Sergio Rosati, Paola Sacchi
Summary: Small ruminant lentiviruses can cause various diseases, and a protective mutation has been identified. However, the protective effect varies across different sheep breeds.
VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Alessia Candellone, Flavia Girolami, Paola Badino, Watanya Jarriyawattanachaikul, Rosangela Odore
Summary: The study found that canine acute enteropathies (AE) could cause oxidative imbalance. Improving the oxidative status of patients, possibly through dietary administration of antioxidants, may support the management of canine AE and reduce the use of antibiotics.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)
