Journal
BIOTECHNIQUES
Volume 65, Issue 5, Pages 275-280Publisher
FUTURE SCI LTD
DOI: 10.2144/btn-2018-0034
Keywords
amplification inhibitors; exogenous control; internal positive control; IPC
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Described here is a novel, universal exogenous internal positive control (IPC), which is fully synthetic for unparalleled quality control. The IPC was rationally designed, is small and efficiently amplified, has been successfully utilized alone or in triplex qPCR reactions, and is not crossreactive to human DNA or to any of the numerous nonhuman DNA samples tested. The IPC was sensitive to inhibition by at least four commonly encountered amplification inhibitors. It was used in a hydrolysis probe qPCR assay; however, it may be adaptable for other applications such as intercalating dye qPCR, PCR, isothermal amplification and reverse transcription-PCR.
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