Cryo-EM structure of the Mre11-Rad50-Nbs1 complex reveals the molecular mechanism of scaffolding functions
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Title
Cryo-EM structure of the Mre11-Rad50-Nbs1 complex reveals the molecular mechanism of scaffolding functions
Authors
Keywords
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Journal
MOLECULAR CELL
Volume 83, Issue 2, Pages 167-185.e9
Publisher
Elsevier BV
Online
2022-12-27
DOI
10.1016/j.molcel.2022.12.003
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Note: Only part of the references are listed.- Mechanism for inverted-repeat recombination induced by a replication fork barrier
- (2022) Léa Marie et al. Nature Communications
- LRET-derived HADDOCK structural models describe the conformational heterogeneity required for DNA cleavage by the Mre11-Rad50 DNA damage repair complex
- (2022) Marella D Canny et al. eLife
- Mre11-Rad50 oligomerization promotes DNA double-strand break repair
- (2022) Vera M. Kissling et al. Nature Communications
- SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells
- (2021) Juri Na et al. Cell Death & Disease
- Mechanism of MRX inhibition by Rif2 at telomeres
- (2021) Florian Roisné-Hamelin et al. Nature Communications
- Highly accurate protein structure prediction with AlphaFold
- (2021) John Jumper et al. NATURE
- Concerted cutting by Spo11 illuminates meiotic DNA break mechanics
- (2021) Dominic Johnson et al. NATURE
- Cellular functions of the protein kinase ATM and their relevance to human disease
- (2021) Ji-Hoon Lee et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- Molecular basis of human ATM kinase inhibition
- (2021) K. Stakyte et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Inhibition of MRN activity by a telomere protein motif
- (2021) Freddy Khayat et al. Nature Communications
- DNA End Resection: Mechanism and Control
- (2021) Petr Cejka et al. Annual Review of Genetics
- Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge
- (2020) Hisashi Tatebe et al. Nature Communications
- Modes of action of the archaeal Mre11/Rad50 DNA-repair complex revealed by fast-scan atomic force microscopy
- (2020) Ekaterina Zabolotnaya et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- The molecular basis and disease relevance of non-homologous DNA end joining
- (2020) Bailin Zhao et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- Dimers of DNA-PK create a stage for DNA double-strand break repair
- (2020) Amanda K. Chaplin et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- A rod conformation of the Pyrococcus furiosus Rad50 coiled coil
- (2020) Young‐Min Soh et al. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
- Topaz-Denoise: general deep denoising models for cryoEM and cryoET
- (2020) Tristan Bepler et al. Nature Communications
- A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization
- (2020) Romain Forey et al. MOLECULAR CELL
- Ctp1 protein-DNA filaments promote DNA Bridging and DNA double-strand break repair
- (2019) Sara N Andres et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
- (2019) Christophe Jung et al. Jove-Journal of Visualized Experiments
- Stepwise 5′ DNA end-specific resection of DNA breaks by the Mre11-Rad50-Xrs2 and Sae2 nuclease ensemble
- (2019) Elda Cannavo et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- The ATP-bound conformation of the Mre11–Rad50 complex is essential for Tel1/ATM activation
- (2019) Corinne Cassani et al. NUCLEIC ACIDS RESEARCH
- A folded conformation of MukBEF and cohesin
- (2019) Frank Bürmann et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- DNA double-strand break repair-pathway choice in somatic mammalian cells
- (2019) Ralph Scully et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex
- (2019) Lisa Käshammer et al. MOLECULAR CELL
- Nej1 Interacts with Mre11 to Regulate Tethering and Dna2 Binding at DNA Double-Strand Breaks
- (2019) Aditya Mojumdar et al. Cell Reports
- Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix
- (2019) Dorothee Liebschner et al. Acta Crystallographica Section D-Structural Biology
- MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment
- (2019) Lei Bian et al. Molecular Cancer
- MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading
- (2019) Axel Delamarre et al. MOLECULAR CELL
- Type II DNA Topoisomerases Cause Spontaneous Double-Strand Breaks in Genomic DNA
- (2019) Morimoto et al. Genes
- The MRE11–RAD50–NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair
- (2018) Aleem Syed et al. Annual Review of Biochemistry
- DNA Repair: The Search for Homology
- (2018) James E. Haber BIOESSAYS
- Homologous recombination and the repair of DNA double-strand breaks
- (2018) William Douglass Wright et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Structurally distinct Mre11 domains mediate MRX functions in resection, end-tethering and DNA damage resistance
- (2018) Corinne Cassani et al. NUCLEIC ACIDS RESEARCH
- True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
- (2018) Christophe Jung et al. Nature Communications
- ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps
- (2018) Tristan Ian Croll Acta Crystallographica Section D-Structural Biology
- 20 Years of Mre11 Biology: No End in Sight
- (2018) Tanya T. Paull MOLECULAR CELL
- OUP accepted manuscript
- (2018) NUCLEIC ACIDS RESEARCH
- A Local Agreement Filtering Algorithm for Transmission EM Reconstructions
- (2018) Kailash Ramlaul et al. JOURNAL OF STRUCTURAL BIOLOGY
- Efficient and robust proteome-wide approaches for cross-linking mass spectrometry
- (2018) Oleg Klykov et al. Nature Protocols
- New tools for automated high-resolution cryo-EM structure determination in RELION-3
- (2018) Jasenko Zivanov et al. eLife
- Plasticity of the Mre11–Rad50–Xrs2–Sae2 nuclease ensemble in the processing of DNA-bound obstacles
- (2017) Weibin Wang et al. GENES & DEVELOPMENT
- Physiological protein blocks direct the Mre11–Rad50–Xrs2 and Sae2 nuclease complex to initiate DNA end resection
- (2017) Giordano Reginato et al. GENES & DEVELOPMENT
- Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair
- (2017) Logan R. Myler et al. MOLECULAR CELL
- Genotoxicity testing: Comparison of the γH2AX focus assay with the alkaline and neutral comet assays
- (2017) Teodora Nikolova et al. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
- MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
- (2017) Shawn Q Zheng et al. NATURE METHODS
- cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
- (2017) Ali Punjani et al. NATURE METHODS
- Biomolecular condensates: organizers of cellular biochemistry
- (2017) Salman F. Banani et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- Eukaryotic Rad50 functions as a rod-shaped dimer
- (2017) Young Bong Park et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Rad50 ATPase activity is regulated by DNA ends and requires coordination of both active sites
- (2017) Rajashree A. Deshpande et al. NUCLEIC ACIDS RESEARCH
- UCSF ChimeraX: Meeting modern challenges in visualization and analysis
- (2017) Thomas D. Goddard et al. PROTEIN SCIENCE
- Eukaryotic Rad50 functions as a rod-shaped dimer
- (2017) Young Bong Park et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression
- (2017) Jun Hyun Kim et al. Cell Reports
- Evaluating In Vitro DNA Damage Using Comet Assay
- (2017) Yanxin Lu et al. Jove-Journal of Visualized Experiments
- Recent developments in the CCP-EM software suite
- (2017) Tom Burnley et al. Acta Crystallographica Section D-Structural Biology
- Structural mechanism of ATP‐dependent DNA binding and DNA end bridging by eukaryotic Rad50
- (2016) Florian Ulrich Seifert et al. EMBO JOURNAL
- Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex
- (2016) Julyun Oh et al. MOLECULAR CELL
- Nbs1 Converts the Human Mre11/Rad50 Nuclease Complex into an Endo/Exonuclease Machine Specific for Protein-DNA Adducts
- (2016) Rajashree A. Deshpande et al. MOLECULAR CELL
- RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together
- (2016) Andrew Seeber et al. MOLECULAR CELL
- ATP‐dependent DNA binding, unwinding, and resection by the Mre11/Rad50 complex
- (2015) Yaqi Liu et al. EMBO JOURNAL
- DNA End Resection: Nucleases Team Up with the Right Partners to Initiate Homologous Recombination
- (2015) Petr Cejka JOURNAL OF BIOLOGICAL CHEMISTRY
- Xlink Analyzer: Software for analysis and visualization of cross-linking data in the context of three-dimensional structures
- (2015) Jan Kosinski et al. JOURNAL OF STRUCTURAL BIOLOGY
- CTFFIND4: Fast and accurate defocus estimation from electron micrographs
- (2015) Alexis Rohou et al. JOURNAL OF STRUCTURAL BIOLOGY
- Interdependence of the Rad50 Hook and Globular Domain Functions
- (2015) Marcel Hohl et al. MOLECULAR CELL
- xVis: a web server for the schematic visualization and interpretation of crosslink-derived spatial restraints
- (2015) Maximilian Grimm et al. NUCLEIC ACIDS RESEARCH
- Structure of the catalytic domain of Mre11 fromChaetomium thermophilum
- (2015) Florian Ulrich Seifert et al. Acta Crystallographica Section F-Structural Biology Communications
- Structure of the Rad50 DNA double‐strand break repair protein in complex with DNA
- (2014) Anna Rojowska et al. EMBO JOURNAL
- RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress
- (2014) Magtouf Gatei et al. HUMAN MOLECULAR GENETICS
- The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High-performance Quadrupole and an Ultra-high-field Orbitrap Analyzer
- (2014) Richard Alexander Scheltema et al. MOLECULAR & CELLULAR PROTEOMICS
- Sae2 promotes dsDNA endonuclease activity within Mre11–Rad50–Xrs2 to resect DNA breaks
- (2014) Elda Cannavo et al. NATURE
- Ataxia Telangiectasia-Mutated (ATM) Kinase Activity Is Regulated by ATP-driven Conformational Changes in the Mre11/Rad50/Nbs1 (MRN) Complex
- (2013) Ji-Hoon Lee et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- The Mre11-Rad50-Nbs1 (MRN) complex has a specific role in the activation of Chk1 in response to stalled replication forks
- (2013) Joon Lee et al. MOLECULAR BIOLOGY OF THE CELL
- Rad50 Zinc Hook Is Important for the Mre11 Complex to Bind Chromosomal DNA Double-stranded Breaks and Initiate Various DNA Damage Responses
- (2012) Jing He et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- A Balance between Tel1 and Rif2 Activities Regulates Nucleolytic Processing and Elongation at Telomeres
- (2012) M. Martina et al. MOLECULAR AND CELLULAR BIOLOGY
- Structure of Mre11–Nbs1 complex yields insights into ataxia-telangiectasia–like disease mutations and DNA damage signaling
- (2012) Christian B Schiller et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- The Mre11:Rad50 Structure Shows an ATP-Dependent Molecular Clamp in DNA Double-Strand Break Repair
- (2011) Katja Lammens et al. CELL
- Crystal structure of the Mre11-Rad50-ATP S complex: understanding the interplay between Mre11 and Rad50
- (2011) H. S. Lim et al. GENES & DEVELOPMENT
- ATM Protein-dependent Phosphorylation of Rad50 Protein Regulates DNA Repair and Cell Cycle Control
- (2011) Magtouf Gatei et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1
- (2011) Valerie Garcia et al. NATURE
- Crystal Structure of Human Mre11: Understanding Tumorigenic Mutations
- (2011) Young Bong Park et al. STRUCTURE
- XDS
- (2010) Wolfgang Kabsch ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Features and development ofCoot
- (2010) P. Emsley et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- The MRN complex in double-strand break repair and telomere maintenance
- (2010) Brandon J. Lamarche et al. FEBS LETTERS
- Reduced Rif2 and lack of Mec1 target short telomeres for elongation rather than double-strand break repair
- (2010) Jean S McGee et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Mre11 Dimers Coordinate DNA End Bridging and Nuclease Processing in Double-Strand-Break Repair
- (2008) R. Scott Williams et al. CELL
- Mre11 Nuclease Activity Has Essential Roles in DNA Repair and Genomic Stability Distinct from ATM Activation
- (2008) Jeffrey Buis et al. CELL
- Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing
- (2008) Eleni P. Mimitou et al. NATURE
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