Ancestral archaea expanded the genetic code with pyrrolysine

The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism

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Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons

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Genetic Incorporation of ϵ‐ N ‐Benzoyllysine by Engineering Methanomethylophilus alvus Pyrrolysyl‐tRNA Synthetase

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A Genetically Encoded Fluorosulfonyloxybenzoyl-l-lysine for Expansive Covalent Bonding of Proteins via SuFEx Chemistry

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Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

(2020) Daniel L. Dunkelmann et al.   Nature Chemistry

Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase

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Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

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New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments

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Methanonatronarchaeum thermophilum gen. nov., sp. nov. and 'Candidatus Methanohalarchaeum thermophilum', extremely halo(natrono)philic methyl-reducing methanogens from hypersaline lakes comprising a new euryarchaeal class Methanonatronarchaeia classis nov.

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Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs

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Error-prone protein synthesis in parasites with the smallest eukaryotic genome

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Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.

(2018) Birthe Meineke et al.   ACS Chemical Biology

An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells

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Loss of protein synthesis quality control in host-restricted organisms

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ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences

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Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase

(2017) Tateki Suzuki et al.   Nature Chemical Biology

tRNAPyl: Structure, function, and applications

(2017) Jeffery M. Tharp et al.   RNA Biology

Genomic exploration of the diversity, ecology, and evolution of the archaeal domain of life

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Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells

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Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1

(2017) Yue Guan et al.   Environmental Microbiology Reports

RNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea

(2017) Takahito Mukai et al.   mBio

Discovery of extremely halophilic, methyl-reducing euryarchaea provides insights into the evolutionary origin of methanogenesis

(2017) Dimitry Y. Sorokin et al.   Nature Microbiology

Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion

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Methylotrophic methanogenesis discovered in the archaeal phylum Verstraetearchaeota

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Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea

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CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes

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Designing logical codon reassignment – Expanding the chemistry in biology

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Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

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Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine

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Expanded Genetic Code Technologies for Incorporating Modified Lysine at Multiple Sites

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Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens: Implications for the Evolution of a Genetic Code Expansion Cassette

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Aminoacylation of tRNA 2′- or 3′-hydroxyl by phosphoseryl- and pyrrolysyl-tRNA synthetases

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Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function

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PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine

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An Asymmetric Synthesis of l-Pyrrolysine

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Functional context, biosynthesis, and genetic encoding of pyrrolysine

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New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0

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SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building

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Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

(2008) Tatsuo Yanagisawa et al.   JOURNAL OF MOLECULAR BIOLOGY

tRNAdb 2009: compilation of tRNA sequences and tRNA genes

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