tRNAPyl: Structure, function, and applications

Expanding the genetic code of Mus musculus

(2017) Songmi Han et al.   Nature Communications

Kinetics of tRNAPyl-mediated amber suppression inEscherichia colitranslation reveals unexpected limiting steps and competing reactions

(2016) Jinfan Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion

(2016) Ana Crnković et al.   CROATICA CHEMICA ACTA

Genetic code expansion in the mouse brain

(2016) Russell J Ernst et al.   Nature Chemical Biology

Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria

(2016) Nanxi Wang et al.   Scientific Reports

The complete genome sequence of the methanogenic archaeon ISO4-H5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales

(2016) Yang Li et al.   Standards in Genomic Sciences

Incorporation of Unnatural Amino Acids in Response to the AGG Codon

(2015) Byeong Sung Lee et al.   ACS Chemical Biology

Ancient horizontal gene transfer and the last common ancestors

(2015) Gregory P Fournier et al.   BMC EVOLUTIONARY BIOLOGY

Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology

(2015) Jiqiang Ling et al.   NATURE REVIEWS MICROBIOLOGY

Rationally evolving tRNAPylfor efficient incorporation of noncanonical amino acids

(2015) Chenguang Fan et al.   NUCLEIC ACIDS RESEARCH

Reassignment of a rare sense codon to a non-canonical amino acid inEscherichia coli

(2015) Takahito Mukai et al.   NUCLEIC ACIDS RESEARCH

Designing logical codon reassignment – Expanding the chemistry in biology

(2015) Anaëlle Dumas et al.   Chemical Science

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

(2015) Joanne M. Ho et al.   ACS Synthetic Biology

New Mode of Energy Metabolism in the Seventh Order of Methanogens as Revealed by Comparative Genome Analysis of “Candidatus Methanoplasma termitum”

(2014) Kristina Lang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Towards Reassigning the Rare AGG Codon inEscherichia coli

(2014) Yu Zeng et al.   CHEMBIOCHEM

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal

(2014) Thomas S Elliott et al.   NATURE BIOTECHNOLOGY

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET

(2014) Kaihang Wang et al.   Nature Chemistry

Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens: Implications for the Evolution of a Genetic Code Expansion Cassette

(2014) Guillaume Borrel et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon

(2013) Wei Niu et al.   ACS Chemical Biology

Transfer RNA Misidentification Scrambles Sense Codon Recoding

(2013) Radha Krishnakumar et al.   CHEMBIOCHEM

Aminoacylation of tRNA 2′- or 3′-hydroxyl by phosphoseryl- and pyrrolysyl-tRNA synthetases

(2013) Markus Englert et al.   FEBS LETTERS

Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion

(2013) Radha Krishnakumar et al.   FEBS LETTERS

Phylogenomic Data Support a Seventh Order of Methylotrophic Methanogens and Provide Insights into the Evolution of Methanogenesis

(2013) Guillaume Borrel et al.   Genome Biology and Evolution

Nonsense and Sense Suppression Abilities of Original and Derivative Methanosarcina mazei Pyrrolysyl-tRNA Synthetase-tRNAPyl Pairs in the Escherichia coli BL21(DE3) Cell Strain

(2013) Keturah A. Odoi et al.   PLoS One

Probing the Limits of Genetic Recoding in Essential Genes

(2013) M. J. Lajoie et al.   SCIENCE

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Reprogramming the Genetic Code: From Triplet to Quadruplet Codes

(2012) Kaihang Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Catalyst-Free and Site-Specific One-Pot Dual-Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

(2012) Bo Wu et al.   CHEMBIOCHEM

Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPylfor genetic code expansion

(2012) Patrick O'Donoghue et al.   FEBS LETTERS

PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine

(2012) Ruisheng Jiang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expanding the genetic code of Drosophila melanogaster

(2012) Ambra Bianco et al.   Nature Chemical Biology

Functional context, biosynthesis, and genetic encoding of pyrrolysine

(2011) Marsha A Gaston et al.   CURRENT OPINION IN MICROBIOLOGY

Expanding the Genetic Code of an Animal

(2011) Sebastian Greiss et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine

(2011) Marsha A. Gaston et al.   NATURE

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding

(2011) J. M. Schrader et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

tRNA Stabilization by Modified Nucleotides

(2010) Yuri Motorin et al.   BIOCHEMISTRY

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

(2010) Susan M. Hancock et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

(2010) Ying Huang et al.   Molecular BioSystems

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

(2009) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

(2009) Peng R. Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Horizontal gene transfer from extinct and extant lineages: biological innovation and the coral of life

(2009) G. P. Fournier et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Structure of Desulfitobacterium hafniense PylSc, a pyrrolysyl-tRNA synthetase

(2008) Marianne M. Lee et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

(2008) Tatsuo Yanagisawa et al.   JOURNAL OF MOLECULAR BIOLOGY

A putative new order of methanogenic Archaea inhabiting the human gut, as revealed by molecular analyses of the mcrA gene

(2008) Agnès Mihajlovski et al.   RESEARCH IN MICROBIOLOGY