Designing logical codon reassignment – Expanding the chemistry in biology

Genetic Incorporation of Histidine Derivatives Using an Engineered Pyrrolysyl-tRNA Synthetase

(2014) Han Xiao et al.   ACS Chemical Biology

Genetic Incorporation of Seven ortho-Substituted Phenylalanine Derivatives

(2014) Jeffery M. Tharp et al.   ACS Chemical Biology

The genetic incorporation of thirteen novel non-canonical amino acids

(2014) Alfred Tuley et al.   CHEMICAL COMMUNICATIONS

Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion

(2014) Nina Bohlke et al.   FEMS MICROBIOLOGY LETTERS

Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells

(2014) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements

(2014) Moritz J. Schmidt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Design of Spiro[2.3]hex-1-ene, a Genetically Encodable Double-Strained Alkene for Superfast Photoclick Chemistry

(2014) Zhipeng Yu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Temporal Dynamics of Transcription, Protein Synthesis, and Secretion during Macrophage Activation

(2014) Katrin Eichelbaum et al.   MOLECULAR & CELLULAR PROTEOMICS

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET

(2014) Kaihang Wang et al.   Nature Chemistry

A Genetically Encoded Acrylamide Functionality

(2013) Yan-Jiun Lee et al.   ACS Chemical Biology

An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon

(2013) Wei Niu et al.   ACS Chemical Biology

Red-Light-Controlled Protein-RNA Crosslinking with a Genetically Encoded Furan

(2013) Moritz J. Schmidt et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Expanding the Genetic Code for Photoclick Chemistry inE. coli, Mammalian Cells, andA. thaliana

(2013) Fahui Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Tryptophanyl-tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis inE. coli

(2013) Abhishek Chatterjee et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Organic Chemistry and Applications for Labeling and Engineering in Live-Cell Systems

(2013) Yousuke Takaoka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Genetic Incorporation of a 2-Naphthol Group into Proteins for Site-Specific Azo Coupling

(2013) Shuo Chen et al.   BIOCONJUGATE CHEMISTRY

Expanding the Library and Substrate Diversity of the Pyrrolysyl-tRNA Synthetase to Incorporate Unnatural Amino Acids Containing Conjugated Rings

(2013) Vanessa K. Lacey et al.   CHEMBIOCHEM

A Click-and-Release Pyrrolysine Analogue

(2013) Marianne M. Lee et al.   CHEMBIOCHEM

Transfer RNA Misidentification Scrambles Sense Codon Recoding

(2013) Radha Krishnakumar et al.   CHEMBIOCHEM

Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion

(2013) Radha Krishnakumar et al.   FEBS LETTERS

A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli

(2013) Akiyoshi Ikeda-Boku et al.   JOURNAL OF BIOCHEMISTRY

Genetically Encoded Fluorescent Probe for the Selective Detection of Peroxynitrite

(2013) Zhi-jie Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded Light-Activated Transcription for Spatiotemporal Control of Gene Expression and Gene Silencing in Mammalian Cells

(2013) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ligand-Free Palladium-Mediated Site-Specific Protein Labeling Inside Gram-Negative Bacterial Pathogens

(2013) Jie Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemical reporters for biological discovery

(2013) Markus Grammel et al.   Nature Chemical Biology

QuaNCAT: quantitating proteome dynamics in primary cells

(2013) Andrew J M Howden et al.   NATURE METHODS

Thiol–yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-l-lysine analogue

(2013) Yiming Li et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Nonsense and Sense Suppression Abilities of Original and Derivative Methanosarcina mazei Pyrrolysyl-tRNA Synthetase-tRNAPyl Pairs in the Escherichia coli BL21(DE3) Cell Strain

(2013) Keturah A. Odoi et al.   PLoS One

Probing the Limits of Genetic Recoding in Essential Genes

(2013) M. J. Lajoie et al.   SCIENCE

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Genetic Incorporation of Twelve meta-Substituted Phenylalanine Derivatives Using a Single Pyrrolysyl-tRNA Synthetase Mutant

(2012) Yane-Shih Wang et al.   ACS Chemical Biology

Expanding the Genetic Code of Caenorhabditis elegans Using Bacterial Aminoacyl-tRNA Synthetase/tRNA Pairs

(2012) Angela R. Parrish et al.   ACS Chemical Biology

Ligation of Expressed Protein α-Hydrazides via Genetic Incorporation of an α-Hydroxy Acid

(2012) Yi-Ming Li et al.   ACS Chemical Biology

Release Factor One Is Nonessential in Escherichia coli

(2012) David B. F. Johnson et al.   ACS Chemical Biology

Biochemical analysis with the expanded genetic lexicon

(2012) Hui-wang Ai   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Reprogramming the Genetic Code: From Triplet to Quadruplet Codes

(2012) Kaihang Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded Cyclopropene Directs Rapid, Photoclick-Chemistry-Mediated Protein Labeling in Mammalian Cells

(2012) Zhipeng Yu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction

(2012) Emine Kaya et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Incorporation of ε-N-Crotonyllysine into Histones

(2012) Chan Hyuk Kim et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Amino Acids for Diels-Alder Reactions in Living Cells

(2012) Tilman Plass et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cover Picture: Champagne and Fireworks:Angewandte ChemieCelebrates Its Birthday (Angew. Chem. Int. Ed. 1/2013)

(2012) Peter Gölitz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile Method to Synthesize Histones with Posttranslational Modification Mimics

(2012) Zhiyong U. Wang et al.   BIOCHEMISTRY

Catalyst-Free and Site-Specific One-Pot Dual-Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

(2012) Bo Wu et al.   CHEMBIOCHEM

Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation

(2012) Annika Borrmann et al.   CHEMBIOCHEM

Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPylfor genetic code expansion

(2012) Patrick O'Donoghue et al.   FEBS LETTERS

Rewiring translation - Genetic code expansion and its applications

(2012) Heinz Neumann   FEBS LETTERS

Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

(2012) Eyal Arbely et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific in Vivo Bioorthogonal Ligation with trans-Cyclooctenes

(2012) Jason L. Seitchik et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions

(2012) Kathrin Lang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

(2012) Yane-Shih Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Wide-range protein photo-crosslinking achieved by a genetically encoded Nε-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety

(2012) Tatsuo Yanagisawa et al.   Molecular BioSystems

Expanding the genetic code of Drosophila melanogaster

(2012) Ambra Bianco et al.   Nature Chemical Biology

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

(2012) Kathrin Lang et al.   Nature Chemistry

Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

(2012) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cytoplasmic ATP Hydrolysis Powers Transport of Lipopolysaccharide Across the Periplasm in E. coli

(2012) S. Okuda et al.   SCIENCE

Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling

(2012) Yiming Li et al.   Chemical Science

A “Tag-and-Modify” Approach to Site-Selective Protein Modification

(2011) Justin M. Chalker et al.   ACCOUNTS OF CHEMICAL RESEARCH

Genetically Encoded Copper-Free Click Chemistry

(2011) Tilman Plass et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In Vivo Incorporation of Multiple Noncanonical Amino Acids into Proteins

(2011) Michael G. Hoesl et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Regulation of chromatin by histone modifications

(2011) Andrew J Bannister et al.   CELL RESEARCH

A readily synthesized cyclic pyrrolysine analogue for site-specific protein “click” labeling

(2011) Ziyang Hao et al.   CHEMICAL COMMUNICATIONS

Palladium-mediated site-selective Suzuki–Miyaura protein modification at genetically encoded aryl halides

(2011) Christopher D. Spicer et al.   CHEMICAL COMMUNICATIONS

Expanding the Genetic Code of an Animal

(2011) Sebastian Greiss et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation

(2011) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

(2011) Yane-Shih Wang et al.   Molecular BioSystems

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance

(2011) Meng Zhang et al.   Nature Chemical Biology

Expanding the Genetic Code of Escherichia coli with Phosphoserine

(2011) H.-S. Park et al.   SCIENCE

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Genetically Encoding Unnatural Amino Acids in Neural Stem Cells and Optically Reporting Voltage-Sensitive Domain Changes in Differentiated Neurons

(2011) Bin Shen et al.   STEM CELLS

In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids

(2010) Sandra Lepthien et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Site-Specific Incorporation of Fluorotyrosines into Proteins inEscherichia coliby Photochemical Disguise

(2010) Bryan J. Wilkins et al.   BIOCHEMISTRY

Nonenzymatic Ubiquitylation

(2010) Tomasz Fekner et al.   CHEMBIOCHEM

Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to “Teflon” Proteins

(2010) Lars Merkel et al.   CHEMBIOCHEM

Bioorthogonal chemistry: recent progress and future directions

(2010) Reyna K. V. Lim et al.   CHEMICAL COMMUNICATIONS

Cu-free click cycloaddition reactions in chemical biology

(2010) John C. Jewett et al.   CHEMICAL SOCIETY REVIEWS

Genetically Directing ɛ-N, N-Dimethyl-l-Lysine in Recombinant Histones

(2010) Duy P. Nguyen et al.   CHEMISTRY & BIOLOGY

N6-(2-(R)-Propargylglycyl)lysine as a Clickable Pyrrolysine Mimic

(2010) Xin Li et al.   Chemistry-An Asian Journal

Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon

(2010) Travis S. Young et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Biosynthetic Route to Photoclick Chemistry on Proteins

(2010) Jiangyun Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

(2010) Susan M. Hancock et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically Encoded Photocontrol of Protein Localization in Mammalian Cells

(2010) Arnaud Gautier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Improving a Natural Enzyme Activity through Incorporation of Unnatural Amino Acids

(2010) Isaac N. Ugwumba et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A genetically encoded photocaged Nε-methyl-l-lysine

(2010) Yane-Shih Wang et al.   Molecular BioSystems

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Codon reassignment in the Escherichia coli genetic code

(2010) T. Mukai et al.   NUCLEIC ACIDS RESEARCH

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA

(2010) Randall A. Hughes et al.   NUCLEIC ACIDS RESEARCH

Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion

(2010) Fumie Iraha et al.   NUCLEIC ACIDS RESEARCH

Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis

(2010) Feng Wang et al.   PLoS One

Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli

(2010) Rolf Furter   PROTEIN SCIENCE

Genetically encoding an aliphatic diazirine for protein photocrosslinking

(2010) Chungjung Chou et al.   Chemical Science

A Pyrrolysine Analogue for Site-Specific Protein Ubiquitination

(2009) Xin Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Pyrrolysine Analogue for Protein Click Chemistry

(2009) Tomasz Fekner et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded Alkenes in Yeast

(2009) Hui-wang Ai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

(2009) Peng R. Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Probing Protein Folding Using Site-Specifically Encoded Unnatural Amino Acids as FRET Donors with Tryptophan

(2009) Shigeki J. Miyake-Stoner et al.   BIOCHEMISTRY

Interpretation ofp-Cyanophenylalanine Fluorescence in Proteins in Terms of Solvent Exposure and Contribution of Side-Chain Quenchers: A Combined Fluorescence, IR and Molecular Dynamics Study

(2009) Humeyra Taskent-Sezgin et al.   BIOCHEMISTRY

Nonnatural Amino Acids for Site-Specific Protein Conjugation

(2009) Albert J. de Graaf et al.   BIOCONJUGATE CHEMISTRY

Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications

(2009) Ying Huang et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Expanding the Genetic Code for Biological Studies

(2009) Qian Wang et al.   CHEMISTRY & BIOLOGY

Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine

(2009) Francis B. Peters et al.   CHEMISTRY & BIOLOGY

Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology

(2009) Justin M. Chalker et al.   Chemistry-An Asian Journal

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetically EncodingNϵ-Methyl-l-lysine in Recombinant Histones

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins inSaccharomyces cerevisiae

(2009) Hyun Soo Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteinsviaa Pyrrolysyl-tRNA Synthetase/tRNACUAPair and Click Chemistry

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhancing the utility of unnatural amino acid synthetases by manipulating broad substrate specificity

(2009) Audrey L. Stokes et al.   Molecular BioSystems

FTIR analysis of GPCR activation using azido probes

(2009) Shixin Ye et al.   Nature Chemical Biology

Genetic Encoding of 3-Iodo-l-Tyrosine in Escherichia coli for Single-Wavelength Anomalous Dispersion Phasing in Protein Crystallography

(2009) Kensaku Sakamoto et al.   STRUCTURE

A Genetically Encoded Boronate-Containing Amino Acid

(2008) Eric Brustad et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A promiscuous aminoacyl-tRNA synthetase that incorporates cysteine, methionine, and alanine homologs into proteins

(2008) Eric Brustad et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Oxidative coupling of peptides to a virus capsid containing unnatural amino acids

(2008) Zachary M. Carrico et al.   CHEMICAL COMMUNICATIONS

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

(2008) Tatsuo Yanagisawa et al.   JOURNAL OF MOLECULAR BIOLOGY

Specificity of Pyrrolysyl-tRNA Synthetase for Pyrrolysine and Pyrrolysine Analogs

(2008) Wen-Tai Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Recognition of Non-α-amino Substrates by Pyrrolysyl-tRNA Synthetase

(2008) Takatsugu Kobayashi et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetically Encoding Protein Oxidative Damage

(2008) Heinz Neumann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In vivoIncorporation of Unnatural Amino Acids to Probe Structure, Dynamics, and Ligand Binding in a Large Protein by Nuclear Magnetic Resonance Spectroscopy

(2008) Susan E. Cellitti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast

(2008) Qian Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer

(2008) Eric M. Brustad et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically encoding Nε-acetyllysine in recombinant proteins

(2008) Heinz Neumann et al.   Nature Chemical Biology

Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog

(2008) K. Oki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Azatryptophans endow proteins with intrinsic blue fluorescence

(2008) S. Lepthien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Addition of an α-Hydroxy Acid to the Genetic Code of Bacteria

(2007) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION