Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering

Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins

(2022) Samagya Banskota et al.   CELL

Two high-fidelity variants: efSaCas9 and SaCas9-HF, which one is better?

(2022) Jineng Lv et al.   GENE THERAPY

The use of new CRISPR tools in cardiovascular research and medicine

(2022) Masataka Nishiga et al.   Nature Reviews Cardiology

Structural basis for mismatch surveillance by CRISPR–Cas9

(2022) Jack P. K. Bravo et al.   NATURE

Antispacer peptide nucleic acids for sequence-specific CRISPR-Cas9 modulation

(2022) Nicholas G Economos et al.   NUCLEIC ACIDS RESEARCH

FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity

(2022) Zifeng Cui et al.   Nature Communications

Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae

(2021) Amit K. Sharma et al.   PLANT BIOTECHNOLOGY JOURNAL

Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing

(2021) Zuyan Lu et al.   CRISPR Journal

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination

(2021) Chaya T L Yuen et al.   NUCLEIC ACIDS RESEARCH

Peptide nucleic acid–dependent artifact can lead to false-positive triplex gene editing signals

(2021) Pui Yan Ho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Peptide Nucleic Acids and Gene Editing: Perspectives on Structure and Repair

(2020) Nicholas G. Economos et al.   MOLECULES

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

(2020) Raviraj Banakar et al.   Rice

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

(2020) Hakim Manghwar et al.   Advanced Science

Highly Parallel Profiling of Cas9 Variant Specificity

(2020) Jonathan L. Schmid-Burgk et al.   MOLECULAR CELL

An engineered ScCas9 with broad PAM range and high specificity and activity

(2020) Pranam Chatterjee et al.   NATURE BIOTECHNOLOGY

Machine learning predicts new anti-CRISPR proteins

(2020) Simon Eitzinger et al.   NUCLEIC ACIDS RESEARCH

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

(2020) Derek Cerchione et al.   PLoS One

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

A Cas9 with PAM recognition for adenine dinucleotides

(2020) Pranam Chatterjee et al.   Nature Communications

A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope

(2020) Ziying Hu et al.   PLOS BIOLOGY

CRISPR system: Discovery, development and off-target detection

(2020) Shengmiao Chen et al.   CELLULAR SIGNALLING

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Prediction of the sequence-specific cleavage activity of Cas9 variants

(2020) Nahye Kim et al.   NATURE BIOTECHNOLOGY

High-fidelity SaCas9 identified by directional screening in human cells

(2020) Haihua Xie et al.   PLOS BIOLOGY

Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing

(2020) Muhammad Naeem et al.   Cells

MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

(2020) Linyuan Ma et al.   Nature Communications

Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing

(2020) Pin Lyu et al.   Life-Basel

Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

(2019) Dacheng Ma et al.   Nature Communications

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

Combined Computational-Experimental Approach to Explore Molecular Mechanism of SaCas9 with Broadened DNA Targeting Range

(2019) Binquan Luan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficient Human Genome Editing Using SaCas9 Ribonucleoprotein Complexes

(2019) Yufei Wang et al.   Biotechnology Journal

Efficient disruption of bcr-abl gene by CRISPR RNA-guided FokI nucleases depresses the oncogenesis of chronic myeloid leukemia cells

(2019) Zhenhong Luo et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing

(2019) Pin Lyu et al.   NUCLEIC ACIDS RESEARCH

Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity

(2019) Yuanyan Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improved double-nicking strategies for COL7A1 editing by homologous recombination

(2019) Thomas Kocher et al.   Molecular Therapy-Nucleic Acids

Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

(2019) Wen Xu et al.   BMC PLANT BIOLOGY

Advances in Detecting and Reducing Off-target Effects Generated by CRISPR-mediated Genome Editing

(2019) Jin-Jing Li et al.   Journal of Genetics and Genomics

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

(2019) Daqi Wang et al.   Nature Communications

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology

(2018) Yoshizumi Ishino et al.   JOURNAL OF BACTERIOLOGY

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes

(2018) Zhen Liang et al.   PLANT BIOTECHNOLOGY JOURNAL

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

(2018) Christopher R. Cromwell et al.   Nature Communications

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

(2018) Christopher A. Vakulskas et al.   NATURE MEDICINE

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

xC as9 expands the scope of genome editing with reduced efficiency in rice

(2018) Junjie Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Orthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing

(2018) Mehmet Fatih Bolukbasi et al.   Nature Communications

Minimal PAM specificity of a highly similar SpCas9 ortholog

(2018) Pranam Chatterjee et al.   Science Advances

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

(2017) Eunji Kim et al.   Nature Communications

Hit and go CAS9 delivered through a lentiviral based self-limiting circuit

(2017) Gianluca Petris et al.   Nature Communications

Structural insights into DNA cleavage activation of CRISPR-Cas9 system

(2017) Cong Huai et al.   Nature Communications

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

Naturally Occurring Off-Switches for CRISPR-Cas9

(2016) April Pawluk et al.   CELL

Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

(2016) Daesik Kim et al.   GENOME RESEARCH

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

(2016) Maximilian Müller et al.   MOLECULAR THERAPY

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

(2016) Hao Yin et al.   NATURE BIOTECHNOLOGY

Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice

(2016) Masafumi Mikami et al.   PLANT AND CELL PHYSIOLOGY

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

(2016) Yuichiro Miyaoka et al.   Scientific Reports

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

(2015) Nicolas Wyvekens et al.   HUMAN GENE THERAPY

Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9

(2015) Taeyoung Koo et al.   MOLECULES AND CELLS

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

(2015) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

DNA-binding-domain fusions enhance the targeting range and precision of Cas9

(2015) Mehmet Fatih Bolukbasi et al.   NATURE METHODS

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Targeted gene editing by transfection ofin vitroreconstitutedStreptococcus thermophilusCas9 nuclease complex

(2015) Monika Glemzaite et al.   RNA Biology

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9

(2015) Satoshi Hara et al.   Scientific Reports

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

(2015) Takuya Sato et al.   Stem Cell Reports

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Genome-wide identification of CRISPR/Cas9 off-targets in human genome

(2014) Jinzhi Duan et al.   CELL RESEARCH

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

(2013) Z. Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE