Structural basis for mismatch surveillance by CRISPR–Cas9

High throughput quantification of short nucleic acid samples by capillary electrophoresis with automated data processing

(2021) Tyler L. Dangerfield et al.   ANALYTICAL BIOCHEMISTRY

Engineering Cas9 for human genome editing

(2021) Ian M. Slaymaker et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

DNA interference states of the hypercompact CRISPR–CasΦ effector

(2021) Patrick Pausch et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Probing the stability of the SpCas9–DNA complex after cleavage

(2021) Pierre Aldag et al.   NUCLEIC ACIDS RESEARCH

The promise and challenge of therapeutic genome editing

(2020) Jennifer A. Doudna   NATURE

Prediction of the sequence-specific cleavage activity of Cas9 variants

(2020) Nahye Kim et al.   NATURE BIOTECHNOLOGY

Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

(2020) Mu-Sen Liu et al.   Nature Communications

Massively parallel kinetic profiling of natural and engineered CRISPR nucleases

(2020) Stephen K. Jones et al.   NATURE BIOTECHNOLOGY

UCSF ChimeraX : Structure visualization for researchers, educators, and developers

(2020) Eric F. Pettersen et al.   PROTEIN SCIENCE

Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2

(2020) Xue Huang et al.   Nature Communications

Catalytic-state structure and engineering of Streptococcus thermophilus Cas9

(2020) Yifei Zhang et al.   Nature Catalysis

Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction

(2020) Ali Punjani et al.   NATURE METHODS

Structure and Dynamics of the CRISPR–Cas9 Catalytic Complex

(2019) Giulia Palermo   Journal of Chemical Information and Modeling

Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases

(2019) Daesik Kim et al.   Annual Review of Biochemistry

Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9

(2019) Xing Zhu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Namdinator – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

(2019) Rune Thomas Kidmose et al.   IUCrJ

Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States

(2019) Wei Sun et al.   MOLECULAR CELL

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

(2018) Digvijay Singh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

(2018) Digvijay Singh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

DNA Unwinding Is the Primary Determinant of CRISPR-Cas9 Activity

(2018) Shanzhong Gong et al.   Cell Reports

ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps

(2018) Tristan Ian Croll   Acta Crystallographica Section D-Structural Biology

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

(2017) Yavuz S. Dagdas et al.   Science Advances

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife