AAA+ proteins: one motor, multiple ways to work

Chaperones directly and efficiently disperse stress-triggered biomolecular condensates

(2022) Haneul Yoo et al.   MOLECULAR CELL

Heterozygous Variants of CLPB are a Cause of Severe Congenital Neutropenia

(2021) Julia T Warren et al.   BLOOD

A conserved strategy for structure change and energy transduction in Hsp104 and other AAA+ protein motors

(2021) Xiang Ye et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DAXX represents a new type of protein-folding enabler

(2021) Liangqian Huang et al.   NATURE

Ultrafast pore-loop dynamics in a AAA+ machine point to a Brownian-ratchet mechanism for protein translocation

(2021) Hisham Mazal et al.   Science Advances

The AAA+ superfamily: a review of the structural and mechanistic principles of these molecular machines

(2021) Yousuf A. Khan et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Structure and function of ClpXP, a AAA+ proteolytic machine powered by probabilistic ATP hydrolysis

(2021) Robert T. Sauer et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Targeted substrate loop insertion by VCP/p97 during PP1 complex disassembly

(2021) Johannes van den Boom et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Processive extrusion of polypeptide loops by a Hsp100 disaggregase

(2020) Mario J. Avellaneda et al.   NATURE

Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein

(2020) Wai Kwan Tang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Non-dominant AAA+ Ring in the ClpAP Protease Functions as an Anti-stalling Motor to Accelerate Protein Unfolding and Translocation

(2020) Hema Chandra Kotamarthi et al.   Cell Reports

Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate

(2020) Xue Fei et al.   eLife

A processive rotary mechanism couples substrate unfolding and proteolysis in the ClpXP degradation machinery

(2020) Zev A Ripstein et al.   eLife

AAA+ proteins

(2020) Thiago V. Seraphim et al.   CURRENT BIOLOGY

Nucleation seed size determines amyloid clearance and establishes a barrier to prion appearance in yeast

(2020) Janice Villali et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Conformational plasticity of the ClpAP AAA+ protease couples protein unfolding and proteolysis

(2020) Kyle E. Lopez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural and kinetic basis for the regulation and potentiation of Hsp104 function

(2020) Xiang Ye et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations

(2020) Ryan R Cupo et al.   eLife

Kinetic Analysis of AAA+ Translocases by Combined Fluorescence and Anisotropy Methods

(2020) Nathaniel W. Scull et al.   BIOPHYSICAL JOURNAL

Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates

(2020) Xue Fei et al.   eLife

TRIM11 Prevents and Reverses Protein Aggregation and Rescues a Mouse Model of Parkinson’s Disease

(2020) Guixin Zhu et al.   Cell Reports

The AAA+ protease ClpXP can easily degrade a 31 and a 52-knotted protein

(2019) Elin M. Sivertsson et al.   Scientific Reports

Spiraling in Control: Structures and Mechanisms of the Hsp104 Disaggregase

(2019) James Shorter et al.   Cold Spring Harbor Perspectives in Biology

Hydrogen exchange reveals Hsp104 architecture, structural dynamics, and energetics in physiological solution

(2019) Xiang Ye et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein translocation by the SecA ATPase occurs by a power‐stroke mechanism

(2019) Marco A Catipovic et al.   EMBO JOURNAL

AAA+ protein-based technologies to counter neurodegenerative disease

(2019) Zachary M. March et al.   BIOPHYSICAL JOURNAL

Hsp104 and Potentiated Variants Can Operate as Distinct Nonprocessive Translocases

(2019) Clarissa L. Durie et al.   BIOPHYSICAL JOURNAL

Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding

(2019) Edward C. Twomey et al.   SCIENCE

Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase

(2019) Alexandrea N. Rizo et al.   Nature Communications

Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases

(2019) Han Han et al.   eLife

Stairway to translocation: AAA+ motor structures reveal the mechanisms of ATP‐dependent substrate translocation

(2019) Stephanie N. Gates et al.   PROTEIN SCIENCE

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

(2018) Lin Guo et al.   CELL

ATP hydrolysis-coupled peptide translocation mechanism of Mycobacterium tuberculosis ClpB

(2018) Hongjun Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events

(2018) Karolina Michalska et al.   STRUCTURE

Knots can impair protein degradation by ATP-dependent proteases

(2017) Álvaro San Martín et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104

(2017) Stephanie N. Gates et al.   SCIENCE

Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing

(2017) Cristina Puchades et al.   SCIENCE

A dominant-negative mutant inhibits multiple prion variants through a common mechanism

(2017) Fen Pei et al.   PLoS Genetics

Examination of ClpB Quaternary Structure and Linkage to Nucleotide Binding

(2016) JiaBei Lin et al.   BIOCHEMISTRY

Mechanistic and Structural Insights into the Prion-Disaggregase Activity of Hsp104

(2016) Elizabeth A. Sweeny et al.   JOURNAL OF MOLECULAR BIOLOGY

Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation

(2016) Adam L Yokom et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

(2016) S. Banerjee et al.   SCIENCE

Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane

(2016) Hui Shi et al.   Nature Communications

Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation

(2016) Adam L Yokom et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Escherichia coliClpB is a non-processive polypeptide translocase

(2015) Tao Li et al.   BIOCHEMICAL JOURNAL

Repurposing Hsp104 to Antagonize Seminal Amyloid and Counter HIV Infection

(2015) Laura M. Castellano et al.   CHEMISTRY & BIOLOGY

The Hsp104 N-Terminal Domain Enables Disaggregase Plasticity and Potentiation

(2015) Elizabeth A. Sweeny et al.   MOLECULAR CELL

Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation

(2015) Bei Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Examination of the dynamic assembly equilibrium forE. coliClpB

(2015) JiaBei Lin et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

ATP binding to neighbouring subunits and intersubunit allosteric coupling underlie proteasomal ATPase function

(2015) Young-Chan Kim et al.   Nature Communications

Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation

(2015) Bei Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Hsp104 Overexpression Cures Saccharomyces cerevisiae [ PSI + ] by Causing Dissolution of the Prion Seeds

(2014) Yang-Nim Park et al.   EUKARYOTIC CELL

Mechanochemical basis of protein degradation by a double-ring AAA+ machine

(2014) Adrian O Olivares et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Examination of polypeptide substrate specificity forEscherichia coliClpB

(2014) Tao Li et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Spatial quality control bypasses cell-based limitations on proteostasis to promote prion curing

(2014) Courtney L Klaips et al.   eLife

Conserved Distal Loop Residues in the Hsp104 and ClpB Middle Domain Contact Nucleotide-binding Domain 2 and Enable Hsp70-dependent Protein Disaggregation

(2013) Morgan E. DeSantis et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Processive ATP-driven Substrate Disassembly by theN-Ethylmaleimide-sensitive Factor (NSF) Molecular Machine

(2013) Daniel J. Cipriano et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

E. coli ClpA Catalyzed Polypeptide Translocation Is Allosterically Controlled by the Protease ClpP

(2013) Justin M. Miller et al.   JOURNAL OF MOLECULAR BIOLOGY

Hsp104 Suppresses Polyglutamine-Induced Degeneration Post Onset in a Drosophila MJD/SCA3 Model

(2013) Mimi Cushman-Nick et al.   PLoS Genetics

Hsp104 Gives Clients the Individual Attention They Need

(2012) Amber N. Murray et al.   CELL

Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients

(2012) Morgan E. DeSantis et al.   CELL

Hsp104 Drives “Protein-Only” Positive Selection of Sup35 Prion Strains Encoding Strong [PSI+]

(2012) Morgan E. DeSantis et al.   CHEMISTRY & BIOLOGY

A tightly regulated molecular toggle controls AAA+ disaggregase

(2012) Yuki Oguchi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Small Heat Shock Proteins Potentiate Amyloid Dissolution by Protein Disaggregases from Yeast and Humans

(2012) Martin L. Duennwald et al.   PLOS BIOLOGY

Single-Molecule Protein Unfolding and Translocation by an ATP-Fueled Proteolytic Machine

(2011) Marie-Eve Aubin-Tam et al.   CELL

ClpX(P) Generates Mechanical Force to Unfold and Translocate Its Protein Substrates

(2011) Rodrigo A. Maillard et al.   CELL

Single-Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics

(2011) Chunlai Chen et al.   MOLECULAR CELL

The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System

(2011) James Shorter   PLoS One

Molecular Mechanism of Polypeptide Translocation Catalyzed by the Escherichia coli ClpA Protein Translocase

(2010) Burki Rajendar et al.   JOURNAL OF MOLECULAR BIOLOGY

Single-molecule stepping and structural dynamics of myosin X

(2010) Yujie Sun et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

TheEscherichia coliClpA Molecular Chaperone Self-Assembles into Tetramers

(2009) P. Keith Veronese et al.   BIOCHEMISTRY

Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions

(2008) James Shorter et al.   EMBO JOURNAL

Hsp104 antagonizes α-synuclein aggregation and reduces dopaminergic degeneration in a rat model of Parkinson disease

(2008) Christophe Lo Bianco et al.   JOURNAL OF CLINICAL INVESTIGATION

Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments

(2008) Tobias Haslberger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The AAA+ superfamily of functionally diverse proteins

(2008) Jamie Snider et al.   GENOME BIOLOGY