Chaperones directly and efficiently disperse stress-triggered biomolecular condensates

Processive extrusion of polypeptide loops by a Hsp100 disaggregase

(2020) Mario J. Avellaneda et al.   NATURE

Bacterial Hsp70 resolves misfolded states and accelerates productive folding of a multi-domain protein

(2020) Rahmi Imamoglu et al.   Nature Communications

Condensation of Ded1p Promotes a Translational Switch from Housekeeping to Stress Protein Production

(2020) Christiane Iserman et al.   CELL

Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation

(2020) Zhenying Liu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An In Vitro Assembly System Identifies Roles for RNA Nucleation and ATP in Yeast Stress Granule Formation

(2020) Kyle Begovich et al.   MOLECULAR CELL

Bayesian modeling reveals metabolite‐dependent ultrasensitivity in the cyanobacterial circadian clock

(2020) Lu Hong et al.   Molecular Systems Biology

Transient intracellular acidification regulates the core transcriptional heat shock response

(2020) Catherine G Triandafillou et al.   eLife

Stress-Induced Translation Inhibition through Rapid Displacement of Scanning Initiation Factors

(2020) Stefan Bresson et al.   MOLECULAR CELL

Subcellular localization of the J-protein Sis1 regulates the heat shock response

(2020) Zoë A. Feder et al.   JOURNAL OF CELL BIOLOGY

Cellular sensing by phase separation: Using the process, not just the products

(2019) Haneul Yoo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of the Hsf1-dependent transcriptome via conserved bipartite contacts with Hsp70 promotes survival in yeast

(2019) Sara Peffer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis for client recognition and activity of Hsp40 chaperones

(2019) Yajun Jiang et al.   SCIENCE

Cytoplasmic protein misfolding titrates Hsp70 to activate nuclear Hsf1

(2019) Anna E Masser et al.   eLife

The Control Centers of Biomolecular Phase Separation: How Membrane Surfaces, PTMs, and Active Processes Regulate Condensation

(2019) Wilton T. Snead et al.   MOLECULAR CELL

Efficient conversion of chemical energy into mechanical work by Hsp70 chaperones

(2019) Salvatore Assenza et al.   eLife

Escherichia coli DnaK Allosterically Modulates ClpB between High- and Low-Peptide Affinity States

(2018) Clarissa L. Durie et al.   BIOCHEMISTRY

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

(2018) Lin Guo et al.   CELL

Cellular Handling of Protein Aggregates by Disaggregation Machines

(2018) Axel Mogk et al.   MOLECULAR CELL

Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins

(2018) Pierre Goloubinoff et al.   Nature Chemical Biology

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery

(2018) Sonja Kroschwald et al.   Cell Reports

Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response

(2018) Joanna Krakowiak et al.   eLife

Cochaperones enable Hsp70 to use ATP energy to stabilize native proteins out of the folding equilibrium

(2018) Huafeng Xu   Scientific Reports

Coordinated Hsp110 and Hsp104 Activities Power Protein Disaggregation in Saccharomyces cerevisiae

(2017) Jayasankar Mohanakrishnan Kaimal et al.   MOLECULAR AND CELLULAR BIOLOGY

The Std1 Activator of the Snf1/AMPK Kinase Controls Glucose Response in Yeast by a Regulated Protein Aggregation

(2017) Kobi Simpson-Lavy et al.   MOLECULAR CELL

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104

(2017) Stephanie N. Gates et al.   SCIENCE

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes

(2017) Nadinath B Nillegoda et al.   eLife

Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions

(2016) Jennifer A. Ream et al.   ANALYTICAL BIOCHEMISTRY

Small heat shock proteins sequester misfolding proteins in near-native conformation for cellular protection and efficient refolding

(2016) Sophia Ungelenk et al.   Nature Communications

Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation

(2016) Agnieszka Kłosowska et al.   eLife

Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation

(2016) Xu Zheng et al.   eLife

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

Reversible, Specific, Active Aggregates of Endogenous Proteins Assemble upon Heat Stress

(2015) Edward W.J. Wallace et al.   CELL

Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress

(2015) Valeria Cherkasov et al.   FEBS LETTERS

Roles of Intramolecular and Intermolecular Interactions in Functional Regulation of the Hsp70 J-protein Co-Chaperone Sis1

(2015) Hyun Young Yu et al.   JOURNAL OF MOLECULAR BIOLOGY

Interplay between E. coli DnaK, ClpB and GrpE during Protein Disaggregation

(2015) Shannon M. Doyle et al.   JOURNAL OF MOLECULAR BIOLOGY

The Hsp104 N-Terminal Domain Enables Disaggregase Plasticity and Potentiation

(2015) Elizabeth A. Sweeny et al.   MOLECULAR CELL

Human Hsp70 Disaggregase Reverses Parkinson’s-Linked α-Synuclein Amyloid Fibrils

(2015) Xuechao Gao et al.   MOLECULAR CELL

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation

(2015) Nadinath B. Nillegoda et al.   NATURE

Differential effects of Ydj1 and Sis1 on Hsp70-mediated clearance of stress granules inSaccharomyces cerevisiae

(2015) Robert W. Walters et al.   RNA

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

(2015) Sonja Kroschwald et al.   eLife

Metabolic and Chaperone Gene Loss Marks the Origin of Animals: Evidence for Hsp104 and Hsp78 Chaperones Sharing Mitochondrial Enzymes as Clients

(2015) Albert J. Erives et al.   PLoS One

Crowding Activates ClpB and Enhances Its Association with DnaK for Efficient Protein Aggregate Reactivation

(2014) Ianire Martín et al.   BIOPHYSICAL JOURNAL

Hsp40s Specify Functions of Hsp104 and Hsp90 Protein Chaperone Machines

(2014) Michael Reidy et al.   PLoS Genetics

Hsp70 chaperones are non-equilibrium machines that achieve ultra-affinity by energy consumption

(2014) Paolo De Los Rios et al.   eLife

Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation

(2014) Marta Carroni et al.   eLife

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

Coordination of Translational Control and Protein Homeostasis during Severe Heat Stress

(2013) Valeria Cherkasov et al.   CURRENT BIOLOGY

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

(2013) Carla P Guimaraes et al.   Nature Protocols

Unraveling the Mechanism of Protein Disaggregation Through a ClpB-DnaK Interaction

(2013) R. Rosenzweig et al.   SCIENCE

CDK-Dependent Hsp70 Phosphorylation Controls G1 Cyclin Abundance and Cell-Cycle Progression

(2012) Andrew W. Truman et al.   CELL

Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients

(2012) Morgan E. DeSantis et al.   CELL

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation

(2012) Heike Rampelt et al.   EMBO JOURNAL

Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces

(2012) Fabian Seyffer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Revisiting the contribution of negative charges on the chaperonin cage wall to the acceleration of protein folding

(2012) F. Motojima et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

FoldEco: A Model for Proteostasis in E. coli

(2012) Evan T. Powers et al.   Cell Reports

Small Heat Shock Proteins Potentiate Amyloid Dissolution by Protein Disaggregases from Yeast and Humans

(2012) Martin L. Duennwald et al.   PLOS BIOLOGY

Unique Peptide Substrate Binding Properties of 110-kDa Heat-shock Protein (Hsp110) Determine Its Distinct Chaperone Activity

(2011) Xinping Xu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System

(2011) James Shorter   PLoS One

The M-Domain Controls Hsp104 Protein Remodeling Activity in an Hsp70/Hsp40-Dependent Manner

(2010) Bernhard Sielaff et al.   JOURNAL OF MOLECULAR BIOLOGY

The kinetic parameters and energy cost of the Hsp70 chaperone as a polypeptide unfoldase

(2010) Sandeep K Sharma et al.   Nature Chemical Biology

The HSP70 chaperone machinery: J proteins as drivers of functional specificity

(2010) Harm H. Kampinga et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast

(2010) K. A. Geiler-Samerotte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein Folding in the Cytoplasm and the Heat Shock Response

(2010) R. M. Vabulas et al.   Cold Spring Harbor Perspectives in Biology

Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging

(2008) R. I. Morimoto   GENES & DEVELOPMENT

Distinct Activities of Escherichia coli Small Heat Shock Proteins IbpA and IbpB Promote Efficient Protein Disaggregation

(2008) Elżbieta Ratajczak et al.   JOURNAL OF MOLECULAR BIOLOGY

Substrate threading through the central pore of the Hsp104 chaperone as a common mechanism for protein disaggregation and prion propagation

(2008) Peter Tessarz et al.   MOLECULAR MICROBIOLOGY

Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments

(2008) Tobias Haslberger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Prion Switching in Response to Environmental Stress

(2008) Jens Tyedmers et al.   PLOS BIOLOGY