Hormone Immunoassay Interference: A 2021 Update

Endogenous anti-streptavidin antibodies causing erroneous laboratory results more common than anticipated

(2021) Louise K. Dahll et al.   SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION

Application of Single-Domain Antibodies (“Nanobodies”) to Laboratory Diagnosis

(2021) Tahir S. Pillay et al.   Annals of Laboratory Medicine

What to do in the event of a suspicion of analytical interference during an immunoassay?

(2020) Cindy Lauro et al.   ANNALES DE BIOLOGIE CLINIQUE

BIOTIN SUPPLEMENTATION CAUSES ERRONEOUS ELEVATIONS OF RESULTS IN SOME COMMERCIAL SERUM 25-HYDROXYVITAMIN D (25OHD) ASSAYS

(2020) GD Carter et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Assessment of biotin interference in thyroid function tests

(2020) Youjia Zhang et al.   MEDICINE

Prevalence of detectable biotin in The Netherlands in relation to risk on immunoassay interference

(2020) A. IJpelaar et al.   CLINICAL BIOCHEMISTRY

Biotin interference: evaluation of a new generation of electrochemiluminescent immunoassays for high-sensitive troponin T and thyroid-stimulating hormone testing

(2020) Samy Mzougui et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Interpretation of thyroid function tests during pregnancy

(2020) W. Edward Visser et al.   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

FGF23 measurement in burosumab-treated patients: an emerging treatment may induce a new analytical interference

(2020) Marie-Liesse Piketty et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Familial dysalbuminaemic hyperthyroxinaemia interferes with current free thyroid hormone immunoassay methods

(2020) Serena Khoo et al.   EUROPEAN JOURNAL OF ENDOCRINOLOGY

Anti-goat antibodies as a rare cause of high gonadotropin levels during menopausal transition

(2020) F. Fruzzetti et al.   GYNECOLOGICAL ENDOCRINOLOGY

The Rise and Fall of High-Dose Biotin to Treat Progressive Multiple Sclerosis

(2020) Carolyn H. Goldschmidt et al.   Neurotherapeutics

Immunoassays disruption by high dose biotin therapy: fair warning for neonatal care pediatricians

(2020) Charles R. Lefevre et al.   PEDIATRIC NEUROLOGY

Sense and nonsense concerning biotin interference in laboratory tests

(2020) Alena Moerman et al.   ACTA CLINICA BELGICA

The Differential Diagnosis of Discrepant Thyroid Function Tests: Insistent Pitfalls and Updated Flow-Chart Based on a Long-Standing Experience

(2020) Irene Campi et al.   Frontiers in Endocrinology

Familial Dysalbuminemic Hyperthyroxinemia: An Underdiagnosed Entity

(2020) Xavier Dieu et al.   Journal of Clinical Medicine

Falsely decreased thyroglobulin levels in a patient with differentiated thyroid carcinoma

(2020) J.J. Hillebrand et al.   CLINICA CHIMICA ACTA

Safety and efficacy of MD1003 (high-dose biotin) in patients with progressive multiple sclerosis (SPI2): a randomised, double-blind, placebo-controlled, phase 3 trial

(2020) Bruce A C Cree et al.   LANCET NEUROLOGY

High-dose biotin in multiple sclerosis: the end of the road

(2020) Jeremias Motte et al.   LANCET NEUROLOGY

Unusual Presentation of a Denys-Drash Syndrome Girl with Undisclosed Assumption of Biotin

(2020) Carla Bizzarri et al.   Journal of Clinical Research in Pediatric Endocrinology

Biotin immunoassay interference: A UK-based prevalence study

(2020) A Sanders et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Pre-analytical mysteries: A case of severe hypervitaminosis D and mild hypercalcaemia

(2020) Emma Whittle et al.   Biochemia Medica

Heterophilic antibody interference with TSH measurement on different immunoassay platforms

(2020) Xinqi Cheng et al.   CLINICA CHIMICA ACTA

Further assessment of the prevalence of biotin supplementation and its impact on risk

(2019) Christina M. Trambas et al.   CLINICAL BIOCHEMISTRY

Assessment of Biotin Supplementation Among Patients in an Outpatient Dermatology Clinic

(2019) Jason J. John et al.   JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY

Erroneous thyroid and steroid hormones profile due to anti-streptavidin antibodies

(2019) Jean-Louis Bayart et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Oestradiol measurement during fulvestrant treatment for breast cancer

(2019) Laura J. Owen et al.   BRITISH JOURNAL OF CANCER

Pharmacological and analytical interference in hormone assays for diagnosis of adrenal incidentaloma

(2019) Antoine-Guy Lopez et al.   ANNALES D ENDOCRINOLOGIE

Biotin interference in immunoassay: a review for the laboratory scientist

(2019) Gordon Avery   ANNALS OF CLINICAL BIOCHEMISTRY

Assessment of complement interference in anti-Müllerian hormone immunoassays

(2019) Katherine A. Turner et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Assessment of free testosterone concentration

(2019) Brian G. Keevil et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

High doses of biotin can interfere with immunoassays that use biotin-strept(avidin) technologies: Implications for individuals with biotin-responsive inherited metabolic disorders

(2019) Barry Wolf   MOLECULAR GENETICS AND METABOLISM

Best practices in mitigating the risk of biotin interference with laboratory testing

(2019) Raffick Bowen et al.   CLINICAL BIOCHEMISTRY

PTH immunoassay interference due to human anti-mouse antibodies in an obese subject with normal parathyroid function

(2019) Matthias Laudes et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Interference of anti-streptavidin antibodies in immunoassays: a very rare phenomenon or a more common finding?

(2019) Nick Verougstraete et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

(2019) Daniel Bergman et al.   Scientific Reports

Biotin supplement interference with immunoassays for parathyroid hormone and 25-hydroxyvitamin D in a patient with metabolic bone disease on maintenance hemodialysis

(2019) Richard A Plasse et al.   Clinical Kidney Journal

EXPERT OPINIONS ON ENDOCRINE TOXICITY INDUCED BY NEW ANTICANCER THERAPIES: PRECAUTIONS TO BE TAKEN IN PERFORMING AND INTERPRETING HORMONAL ASSAYS UNDER IMMUNOTHERAPY

(2018) Najiba Lahlou et al.   ANNALES D ENDOCRINOLOGIE

A diagnostic curiosity of isolated androstenedione elevation due to autoantibodies against horseradish peroxidase label of the immunoassay

(2018) Yvonne Yijuan Lim et al.   CLINICA CHIMICA ACTA

Fake news? Biotin interference in thyroid immunoassays

(2018) Viktoria F. Koehler et al.   CLINICA CHIMICA ACTA

Spurious testosterone laboratory results in a patient taking synthetic alkaline phosphatase (asfotase alfa)

(2018) Alina G. Sofronescu et al.   CLINICAL BIOCHEMISTRY

Prevalence of biotin supplement usage in outpatients and plasma biotin concentrations in patients presenting to the emergency department

(2018) Brooke M. Katzman et al.   CLINICAL BIOCHEMISTRY

Anti-streptavidin antibodies mimicking heterophilic antibodies in thyroid function tests

(2018) Julien Favresse et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Measuring procalcitonin to overcome heterophilic-antibody-induced spurious hypercalcitoninemia

(2018) Luca Giovanella et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Observational studies on macroprolactin in a routine clinical laboratory

(2018) Julian H. Barth et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Anti-streptavidin IgG antibody interference in anti-cyclic citrullinated peptide (CCP) IgG antibody assays is a rare but important cause of false-positive anti-CCP results

(2018) Mario Berth et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Immunoassay interference secondary to therapeutic high-dose biotin: A paediatric case report

(2018) Natalie Evans et al.   JOURNAL OF PAEDIATRICS AND CHILD HEALTH

Apparent Hyperthyroidism Caused by Biotin-Like Interference from IgM Anti-Streptavidin Antibodies

(2018) Leo Lam et al.   THYROID

a case of laboratory-generated “thyroid dysfunction”

(2018) Véronique Raverot et al.   ANNALES D ENDOCRINOLOGIE

Strategies for mitigating risk posed by biotin interference on clinical immunoassays

(2018) Jessica L. Gifford et al.   CLINICAL BIOCHEMISTRY

French Endocrine Society Guidance on endocrine side effects of immunotherapy

(2018) F Castinetti et al.   ENDOCRINE-RELATED CANCER

Insuffisance rénale chronique et biotine : un duo propice aux bilans thyroïdiens et parathyroïdiens erronés, à propos de 2 cas

(2017) Miora Koloina Ranaivosoa et al.   Nephrologie & Therapeutique

A Single 10 mg Oral Dose of Biotin Interferes with Thyroid Function Tests

(2017) Rosa Paula M. Biscolla et al.   THYROID

Thyroxine (T4) Autoantibody Interference of Free T4 Concentration Measurement in a Patient With Hashimoto's Thyroiditis

(2017) Mi-Na Lee et al.   Annals of Laboratory Medicine

Clinical, Genetic, and Protein Structural Aspects of Familial Dysalbuminemic Hyperthyroxinemia and Hypertriiodothyroninemia

(2017) Ulrich Kragh-Hansen et al.   Frontiers in Endocrinology

Artefactual 25-OH vitamin D concentration in multiple myeloma

(2017) Michal WS Ong et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin–biotin immunoassays

(2017) Christina Trambas et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Immunoassay interference complicating management of Cushing’s disease: the onus is on the clinician and the laboratory

(2017) Kay W Choy et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Performance evaluation of an automated electrochemiluminescent calcitonin (CT) immunoassay in diagnosis of medullary thyroid carcinoma

(2016) Johan Schiettecatte et al.   CLINICAL BIOCHEMISTRY

Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance

(2016) J. M. Hawley et al.   CLINICAL CHEMISTRY

The insulin autoimmune syndrome (IAS) as a cause of hypoglycaemia: an update on the pathophysiology, biochemical investigations and diagnosis

(2016) Adel A.A. Ismail   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

A possible cause of the variable detectability of macroprolactin by different immunoassay systems

(2016) Naoki Hattori et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Anti-streptavidin interferences in Roche thyroid immunoassays: a case report

(2016) Lucas Peltier et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Proopiomelanocortin interference in the measurement of adrenocorticotrophic hormone: a United Kingdom National External Quality Assessment Service study

(2016) P.J. Monaghan et al.   CLINICAL ENDOCRINOLOGY

Pharmacokinetics and pharmacodynamics of MD1003 (high-dose biotin) in the treatment of progressive multiple sclerosis

(2016) Laure Peyro Saint Paul et al.   Expert Opinion on Drug Metabolism & Toxicology

Factitious Graves' Disease Due to Biotin Immunoassay Interference—A Case and Review of the Literature

(2016) Marianne S. Elston et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Biotin Treatment Mimicking Graves’ Disease

(2016) Sebastian Kummer et al.   NEW ENGLAND JOURNAL OF MEDICINE

Heterophilic antibody interference affecting multiple hormone assays: Is it due to rheumatoid factor?

(2016) Shiva Mongolu et al.   SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION

Misdiagnosis of Graves' Disease with Apparent Severe Hyperthyroidism in a Patient Taking Biotin Megadoses

(2016) Giuseppe Barbesino   THYROID

2015 American Thyroid Association Management Guidelines for Adult Patients with Thyroid Nodules and Differentiated Thyroid Cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer

(2016) Bryan R. Haugen et al.   THYROID

Falsely elevated serum oestradiol due to exemestane therapy

(2016) Sanja Mandic et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Correlation of Serum Thyroid Hormones Autoantibodies with Self-Reported Exposure to Thyroid Disruptors in a Group of Nonsegmental Vitiligo Patients

(2015) Roberta Colucci et al.   ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

Inherited defects of thyroxine-binding proteins

(2015) Theodora Pappa et al.   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

False positive in the measurement of thyroglobulin induced by rheumatoid factor

(2015) G. Astarita et al.   CLINICA CHIMICA ACTA

Commercial insulin immunoassays fail to detect commonly prescribed insulin analogues

(2015) C. Parfitt et al.   CLINICAL BIOCHEMISTRY

Thyroglobulin measurement by highly sensitive assays: focus on laboratory challenges

(2015) Luca Giovanella et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Variability in the detection of macro TSH in different immunoassay systems

(2015) Naoki Hattori et al.   EUROPEAN JOURNAL OF ENDOCRINOLOGY

A Case With Immunoassay Interferences in the Measurement of Multiple Hormones

(2015) Ozlem Gulbahar et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Gestational Trophoblastic Disease Diagnosis Delayed by the Hook Effect

(2015) Julia Cormano et al.   OBSTETRICS AND GYNECOLOGY

Cross-reactivity of insulin analogues with three insulin assays

(2015) A Dayaldasani et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Endogenous Antibody Interferences in Immunoassays

(2015) Jane F. Emerson et al.   Labmedicine

Interference in thyroid hormones with Roche immunoassays: an unfinished story

(2014) Martina Zaninotto et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Species cross-reactivity of rheumatoid factors and implications for immunoassays

(2014) Bettina E. Holm et al.   SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION

How sensitive (second-generation) thyroglobulin measurement is changing paradigms for monitoring patients with differentiated thyroid cancer, in the absence or presence of thyroglobulin autoantibodies

(2014) Carole Spencer et al.   Current Opinion in Endocrinology Diabetes and Obesity

Interference From Anti-Streptavidin Antibody

(2013) Nichole Johnson Rulander et al.   ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE

External quality assessment of hormone determinations

(2013) Catharine M. Sturgeon   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

Principles and pitfalls of free hormone measurements

(2013) James D. Faix   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

Determination of prolactin: The macroprolactin problem

(2013) Michael Fahie-Wilson et al.   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

Heterophilic antibody interference in immunometric assays

(2013) Nils Bolstad et al.   BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM

Recurrent Pituitary Macroadenoma with Increased Plasma ACTH Precursors that Cross-React in a Commonly Used ACTH Immunoassay

(2013) Kenneth Woeber et al.   Endocrine Practice

Falsely Undetectable TSH in a Cohort of South Asian Euthyroid Patients

(2013) Julia C. Drees et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Automated 22-kD Growth Hormone-Specific Assay without Interference from Pegvisomant

(2012) J. Manolopoulou et al.   CLINICAL CHEMISTRY

Accuracy of First and Second Generation Testosterone Assays and Improvement through Sample Extraction

(2012) W. M. T. Groenestege et al.   CLINICAL CHEMISTRY

Falsely elevated thyroid hormone levels caused by anti-ruthenium interference in the Elecsys assay resembling the syndrome of inappropriate secretion of thyrotropin

(2012) Kenji Ohba et al.   ENDOCRINE JOURNAL

Detecting and solving the interference of pregnancy serum, in a GH immunometric assay

(2012) Monike L. Dias et al.   GROWTH HORMONE & IGF RESEARCH

Macro-Thyrotropin: A Case Report and Review of Literature

(2012) Tze Ping Loh et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Elevated thyroid-stimulating hormone level in a euthyroid neonate caused by macro thyrotropin-IgG complex

(2011) Mariane Rix et al.   ACTA PAEDIATRICA

Measured Dehydroepiandrosterone Sulfate Positively Influences Testosterone Measurement in Unextracted Female Serum: Comparison of 2 Immunoassays with Testosterone Measured by LC-MS

(2011) S. C. Benton et al.   CLINICAL CHEMISTRY

What should be done when thyroid function tests do not make sense?

(2011) Mark Gurnell et al.   CLINICAL ENDOCRINOLOGY

Analytical error and interference in immunoassay: minimizing risk

(2011) C. M. Sturgeon et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Interference by antiruthenium antibodies in the Roche thyroid-stimulating hormone assay

(2011) M. M. Buijs et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Spuriously High Free Thyroxine Values in Familial Dysalbuminemic Hyperthyroxinemia

(2010) H. A. Ross et al.   CLINICAL CHEMISTRY

Interference in thyroid-stimulating hormone determination

(2010) Mauro Imperiali et al.   EUROPEAN JOURNAL OF CLINICAL INVESTIGATION

Steroid Assays in Paediatric Endocrinology

(2010) John W. Honour   Journal of Clinical Research in Pediatric Endocrinology

Immunoglobulin interference in serum follicle-stimulating hormone assays: autoimmune and heterophilic antibody interference

(2010) R. Webster et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Prevalence of human anti-mouse antibodies (HAMAs) in routine examinations

(2009) Saori Koshida et al.   CLINICA CHIMICA ACTA

Familial Dysalbuminemic Hyperthyroxinemia: A Persistent Diagnostic Challenge

(2009) D. Cartwright et al.   CLINICAL CHEMISTRY

A Case of Renal Osteodystrophy with Unexpected Serum Intact Parathyroid Hormone Concentrations

(2009) D. L. Meany et al.   CLINICAL CHEMISTRY

Heterophilic antibodies may be a cause of falsely low total IGF1 levels

(2009) M P Brugts et al.   EUROPEAN JOURNAL OF ENDOCRINOLOGY

Interference from endogenous antibodies in automated immunoassays: what laboratorians need to know

(2009) A A A Ismail   JOURNAL OF CLINICAL PATHOLOGY

Autoimmune Forms of Hypoglycemia

(2009) Beatrice C. Lupsa et al.   MEDICINE

A patient with pseudo-Addison's disease and falsely elevated thyroxine due to interference in serum cortisol and free thyroxine immunoassays by two different mechanisms

(2009) M. Saleem et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Two cases of antiruthenium antibody interference in Modular free thyroxine assay

(2009) A. C Heijboer et al.   ANNALS OF CLINICAL BIOCHEMISTRY

Anti-thyroxine and anti-triiodothyronine antibody interferences in one-step free triiodothyronine and free thyroxine immunoassays

(2008) Catherine Massart et al.   CLINICA CHIMICA ACTA

Influence of Thyroid Hormone Autoantibodies on 7 Thyroid Hormone Assays

(2008) S. A. Zouwail et al.   CLINICAL CHEMISTRY

Euthyroid Patient with Elevated Serum Free Thyroxine

(2008) G. van der Watt et al.   CLINICAL CHEMISTRY

When is elevated testosterone not testosterone? When it is an immunoassay interfering antibody

(2008) D RAMAEKER et al.   FERTILITY AND STERILITY

Transient human anti-mouse antibodies (HAMA) interference in CA 125 measurements during monitoring of ovarian cancer patients treated with murine monoclonal antibody

(2008) Angèle L.M. Oei et al.   GYNECOLOGIC ONCOLOGY

Polyethylene glycol precipitation: proceed with care

(2008) M. Fahie-Wilson et al.   ANNALS OF CLINICAL BIOCHEMISTRY

False positive PTH results: An easy strategy to test and detect analytical interferences in routine practice

(2007) Etienne Cavalier et al.   CLINICA CHIMICA ACTA