Synthetic biology enables field-deployable biosensors for water contaminants

Wastewater-based epidemiology—surveillance and early detection of waterborne pathogens with a focus on SARS-CoV-2, Cryptosporidium and Giardia

(2021) Alireza Zahedi et al.   PARASITOLOGY RESEARCH

A glucose meter interface for point-of-care gene circuit-based diagnostics

(2021) Evan Amalfitano et al.   Nature Communications

A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors

(2021) Brent Townshend et al.   Nature Communications

Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences

(2021) Ilenne Del Valle et al.   Frontiers in Microbiology

Hydrogel-based biocontainment of bacteria for continuous sensing and computation

(2021) Tzu-Chieh Tang et al.   Nature Chemical Biology

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

(2021) Kean Hean Ooi et al.   Nature Communications

Immobilization of Proteins of Cell Extract to Hydrogel Networks Enhances the Longevity of Cell-Free Protein Synthesis and Supports Gene Networks

(2021) Xiaofei Ouyang et al.   ACS Synthetic Biology

Programming living sensors for environment, health and biomanufacturing

(2021) Xinyi Wan et al.   Microbial Biotechnology

SARS-CoV-2 Detection with De Novo-Designed Synthetic Riboregulators

(2021) İlkay Çisil Köksaldı et al.   ANALYTICAL CHEMISTRY

Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics

(2021) Chenqi Niu et al.   BIOSENSORS & BIOELECTRONICS

Programmable receptors enable bacterial biosensors to detect pathological biomarkers in clinical samples

(2021) Hung-Ju Chang et al.   Nature Communications

CRISPR-based diagnostics

(2021) Michael M. Kaminski et al.   Nature Biomedical Engineering

Microbial whole-cell biosensors: Current applications, challenges, and future perspectives

(2021) Michael Moraskie et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-Cas based virus detection: Recent advances and perspectives

(2021) Lijuan Yin et al.   BIOSENSORS & BIOELECTRONICS

Genome-scale transcriptional dynamics and environmental biosensing

(2020) Garrett Graham et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology

(2020) Catherine Fan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Can a Paper-Based Device Trace COVID-19 Sources with Wastewater-Based Epidemiology?

(2020) Kang Mao et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection

(2020) Lei Peng et al.   SENSORS AND ACTUATORS B-CHEMICAL

Applying CRISPR-Cas12a as a Signal Amplifier to Construct Biosensors for Non-DNA Targets in Ultralow Concentrations

(2020) Junjie Li et al.   ACS Sensors

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

(2020) Liyuan Zhang et al.   Biotechnology Journal

Cell-free biosensors for rapid detection of water contaminants

(2020) Jaeyoung K. Jung et al.   NATURE BIOTECHNOLOGY

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

SPRINT: a Cas13a-based platform for detection of small molecules

(2020) Roman S Iwasaki et al.   NUCLEIC ACIDS RESEARCH

Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofPlasmodiumspecies in symptomatic and asymptomatic malaria

(2020) Rose A. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

(2020) Kayla G. Barnes et al.   Nature Communications

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Rethinking wastewater risks and monitoring in light of the COVID-19 pandemic

(2020) Anne Bogler et al.   Nature Sustainability

Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

(2020) Bo Pang et al.   ANALYTICAL CHEMISTRY

Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity

(2020) Xinyi Wan et al.   Nature Communications

Versatile detection with CRISPR/Cas system from applications to challenges

(2020) Hui Wu et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Biosensors for wastewater-based epidemiology for monitoring public health

(2020) Kang Mao et al.   WATER RESEARCH

A simple and low-cost paper-based colorimetric method for detecting and distinguishing the GII.4 and GII.17 genotypes of norovirus

(2020) Qiuli Sun et al.   TALANTA

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals

(2019) Xinyi Wan et al.   Nature Chemical Biology

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Comprehensive Profiling of Diverse Genetic Reporters with Application to Whole-Cell and Cell-Free Biosensors

(2019) Antonia Lopreside et al.   ANALYTICAL CHEMISTRY

Synthetic Biology Enables Programmable Cell‐Based Biosensors

(2019) Maggie Hicks et al.   CHEMPHYSCHEM

Engineering orthogonal signalling pathways reveals the sparse occupancy of sequence space

(2019) Conor J. McClune et al.   NATURE

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

(2019) Daqi Wang et al.   Nature Communications

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

Genetic Circuits To Detect Nanomaterial Triggered Toxicity through Engineered Heat Shock Response Mechanism

(2019) Behide Saltepe et al.   ACS Synthetic Biology

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

(2019) Ying Xiong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Programmable Inhibition and Detection of RNA Viruses Using Cas13

(2019) Catherine A. Freije et al.   MOLECULAR CELL

A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a

(2019) Florian Katzmeier et al.   PLoS One

Design rules of synthetic non-coding RNAs in bacteria

(2018) Young Je Lee et al.   METHODS

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Cellular Biosensors with Engineered Genetic Circuits

(2017) Behide Saltepe et al.   ACS Sensors

Microbially derived biosensors for diagnosis, monitoring and epidemiology

(2017) Hung-Ju Chang et al.   Microbial Biotechnology

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya and dengue viruses

(2017) Aashish Priye et al.   Scientific Reports

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors

(2016) Catherine Brutesco et al.   ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

A sensitive whole-cell biosensor for the simultaneous detection of a broad-spectrum of toxic heavy metal ions

(2015) S. Cerminati et al.   CHEMICAL COMMUNICATIONS

Community Sewage Sensors for Monitoring Public Health

(2015) Zhugen Yang et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

Biocontainment of genetically modified organisms by synthetic protein design

(2015) Daniel J. Mandell et al.   NATURE

'Deadman' and 'Passcode' microbial kill switches for bacterial containment

(2015) Clement T Y Chan et al.   Nature Chemical Biology

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities

(2015) Baojun Wang et al.   NUCLEIC ACIDS RESEARCH

Multilayered genetic safeguards limit growth of microorganisms to defined environments

(2015) Ryan R. Gallagher et al.   NUCLEIC ACIDS RESEARCH

Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates

(2015) Alexis Courbet et al.   Science Translational Medicine

Innovative biological approaches for monitoring and improving water quality

(2015) Sanja Aracic et al.   Frontiers in Microbiology

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Rapid engineering of versatile molecular logic gates using heterologous genetic transcriptional modules

(2014) Baojun Wang et al.   CHEMICAL COMMUNICATIONS

Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks

(2014) Baojun Wang et al.   NUCLEIC ACIDS RESEARCH

GeneGuard: A Modular Plasmid System Designed for Biosafety

(2014) Oliver Wright et al.   ACS Synthetic Biology

Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery

(2013) Miso Park et al.   SENSORS

A modular cell-based biosensor using engineered genetic logic circuits to detect and integrate multiple environmental signals

(2012) Baojun Wang et al.   BIOSENSORS & BIOELECTRONICS

Customizing cell signaling using engineered genetic logic circuits

(2012) Baojun Wang et al.   TRENDS IN MICROBIOLOGY

Design and Application of a Mevalonate-Responsive Regulatory Protein

(2010) Shuang-Yan Tang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Where microbiology meets microengineering: design and applications of reporter bacteria

(2010) Jan Roelof van der Meer et al.   NATURE REVIEWS MICROBIOLOGY