An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

A smartphone-read ultrasensitive and quantitative saliva test for COVID-19

(2021) Bo Ning et al.   Science Advances

Presumed Asymptomatic Carrier Transmission of COVID-19

(2020) Yan Bai et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

A novel coronavirus outbreak of global health concern

(2020) Chen Wang et al.   LANCET

A pneumonia outbreak associated with a new coronavirus of probable bat origin

(2020) Peng Zhou et al.   NATURE

Transmission of 2019-nCoV Infection from an Asymptomatic Contact in Germany

(2020) Camilla Rothe et al.   NEW ENGLAND JOURNAL OF MEDICINE

A Novel Coronavirus from Patients with Pneumonia in China, 2019

(2020) Na Zhu et al.   NEW ENGLAND JOURNAL OF MEDICINE

Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia

(2020) Qun Li et al.   NEW ENGLAND JOURNAL OF MEDICINE

The Architecture of SARS-CoV-2 Transcriptome

(2020) Dongwan Kim et al.   CELL

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

(2020) Xinjie Wang et al.   Science Bulletin

Evidence for host-dependent RNA editing in the transcriptome of SARS-CoV-2

(2020) Salvatore Di Giorgio et al.   Science Advances

SARS-CoV-2 detection with CRISPR diagnostics

(2020) Lu Guo et al.   Cell Discovery

Emergence of Drift Variants That May Affect COVID-19 Vaccine Development and Antibody Treatment

(2020) Takahiko Koyama et al.   Pathogens

Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride

(2020) Yinhua Zhang et al.   BIOTECHNIQUES

Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification

(2020) Matthew A Lalli et al.   CLINICAL CHEMISTRY

Emergence of genomic diversity and recurrent mutations in SARS-CoV-2

(2020) Lucy van Dorp et al.   INFECTION GENETICS AND EVOLUTION

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

SNPs or RNA modifications? Concerns on mutation-based evolutionary studies of SARS-CoV-2

(2020) Yue Li et al.   PLoS One

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

(2020) Long T. Nguyen et al.   Nature Communications

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

(2020) Tieying Hou et al.   PLoS Pathogens

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer

(2020) Victoria A. Avanzato et al.   CELL

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

(2020) Dan Xiong et al.   PLOS BIOLOGY

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses

(2019) Yi Zhou et al.   Frontiers in Microbiology

Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells

(2018) Moira A. McMahon et al.   MOLECULAR THERAPY

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

(2018) Hao Yin et al.   Nature Chemical Biology

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

(2018) Aamir Mir et al.   Nature Communications

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

(2018) Su Bin Moon et al.   Nature Communications

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

(2018) Hyo Min Park et al.   Nature Communications

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

GISAID: Global initiative on sharing all influenza data – from vision to reality

(2017) Yuelong Shu et al.   Eurosurveillance

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

(2017) Hao Yin et al.   NATURE BIOTECHNOLOGY

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Improved Performance of Loop-Mediated Isothermal Amplification Assays via Swarm Priming

(2016) Rhett L. Martineau et al.   ANALYTICAL CHEMISTRY

Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA

(2016) Takashi Yamano et al.   CELL

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

(2016) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

(2015) De-Guo Wang et al.   MOLECULES

Synthetic CRISPR RNA-Cas9–guided genome editing in human cells

(2015) Meghdad Rahdar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Loop-Mediated Amplification Accelerated by Stem Primers

(2011) Olga Gandelman et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES