Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

Design and Optimization of a Cell-Free Atrazine Biosensor

(2020) Adam D. Silverman et al.   ACS Synthetic Biology

The epidemiology, diagnosis and treatment of COVID-19

(2020) Pan Zhai et al.   INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

Biological Materials: The Next Frontier for Cell-Free Synthetic Biology

(2020) Richard J. R. Kelwick et al.   Frontiers in Bioengineering and Biotechnology

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

A simple, robust, and low-cost method to produce the PURE cell-free system.

(2019) Barbora Lavickova et al.   ACS Synthetic Biology

A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs

(2019) Alexander Gräwe et al.   PLoS One

Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals

(2019) Xinyi Wan et al.   Nature Chemical Biology

Interfacing a Personal Glucose Meter with Cell-Free Protein Synthesis for Rapid Analysis of Amino Acids

(2019) Yeon-Jae Jang et al.   ANALYTICAL CHEMISTRY

Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study

(2019) Hideyuki Matsuura et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Detection of inorganic ions and organic molecules with cell-free biosensing systems

(2019) Peng Zhang et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR/Cas Powered Multiplexed Biosensing

(2019) Richard Bruch et al.   TRENDS IN BIOTECHNOLOGY

CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors

(2019) Peter L. Voyvodic et al.   Nature Communications

On a Robust, Sensitive Cell-Free Method for Pseudomonas Sensing and Quantification in Microfluidic Templated Hydrogels

(2019) Jong Seto   Micromachines

Development of a Cell-Free Optical Biosensor for Detection of a Broad Range of Mercury Contaminants in Water: A Plasmid DNA-Based Approach

(2019) Saurabh Gupta et al.   ACS Omega

Optimizing Cell-Free Biosensors to Monitor Enzymatic Production

(2019) Amir Pandi et al.   ACS Synthetic Biology

Quantification of Interlaboratory Cell-Free Protein Synthesis Variability

(2019) Stephanie D. Cole et al.   ACS Synthetic Biology

Comprehensive Profiling of Diverse Genetic Reporters with Application to Whole-Cell and Cell-Free Biosensors

(2019) Antonia Lopreside et al.   ANALYTICAL CHEMISTRY

Synthetic Biology Enables Programmable Cell‐Based Biosensors

(2019) Maggie Hicks et al.   CHEMPHYSCHEM

Custom-made transcriptional biosensors for metabolic engineering

(2019) Mathilde Koch et al.   CURRENT OPINION IN BIOTECHNOLOGY

A multiplexed, electrochemical interface for gene-circuit-based sensors

(2019) Peivand Sadat Mousavi et al.   Nature Chemistry

d-amino Acids in Health and Disease: A Focus on Cancer

(2019) Jacco J.A.J. Bastings et al.   Nutrients

In Vitro Use of Cellular Synthetic Machinery for Biosensing Applications

(2019) Kyung-Ho Lee et al.   Frontiers in Pharmacology

Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid

(2019) Xiangyang Liu et al.   ACS Synthetic Biology

Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor

(2019) Walter Thavarajah et al.   ACS Synthetic Biology

Reengineering Cell-Free Protein Synthesis as a Biosensor: Biosensing with Transcription, Translation, and Protein-folding

(2018) Mehran Soltani et al.   BIOCHEMICAL ENGINEERING JOURNAL

Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization

(2018) Keith Pardee   BIOCHEMICAL ENGINEERING JOURNAL

Transcription Factor-Based Biosensors in High-Throughput Screening: Advances and Applications

(2018) Feng Cheng et al.   Biotechnology Journal

Spinach-based fluorescent light-up biosensors for multiplexed and label-free detection of microRNAs

(2018) Zhan-Ming Ying et al.   CHEMICAL COMMUNICATIONS

Smelling Pseudomonas aeruginosa infections using a whole-cell biosensor – An alternative for the gold-standard culturing assay

(2018) Igor Kviatkovski et al.   JOURNAL OF BIOTECHNOLOGY

Membrane protein-based biosensors

(2018) Nobuo Misawa et al.   Journal of the Royal Society Interface

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

(2018) Ryan Marshall et al.   MOLECULAR CELL

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach

(2018) Amin S.M. Salehi et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials

(2018) Ana I. Benítez-Mateos et al.   ACS Synthetic Biology

Transcription Factor-Based Biosensors in High-Throughput Screening: Advances and Applications

(2018) Feng Cheng et al.   Biotechnology Journal

Functional expression of olfactory receptors using cell-free expression system for biomimetic sensors towards odorant detection

(2018) Fangming Chen et al.   BIOSENSORS & BIOELECTRONICS

Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater

(2018) C. Schirmer et al.   JOURNAL OF BIOTECHNOLOGY

BioBits™ Bright: A fluorescent synthetic biology education kit

(2018) Jessica C. Stark et al.   Science Advances

Biosensor‐Assisted Engineering of a High‐Yield Pichia pastoris Cell‐Free Protein Synthesis Platform

(2018) Rochelle Aw et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cascading Amplification of Immunoassay Signal by Cell-Free Expression of Firefly Luciferase from Detection Antibody-Conjugated DNA in an Escherichia coli Extract

(2018) Ju-Young Byun et al.   ACS Sensors

Paper-based colorimetric biosensor for antibiotics inhibiting bacterial protein synthesis

(2017) Tran Thi My Duyen et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Prevalence of exposure of heavy metals and their impact on health consequences

(2017) Kanwal Rehman et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Preservation of protein expression systems at elevated temperatures for portable therapeutic production

(2017) David K. Karig et al.   Journal of the Royal Society Interface

Progress in and promise of bacterial quorum sensing research

(2017) Marvin Whiteley et al.   NATURE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Exposure to pesticides and the associated human health effects

(2017) Ki-Hyun Kim et al.   SCIENCE OF THE TOTAL ENVIRONMENT

The Application of Whole Cell-Based Biosensors for Use in Environmental Analysis and in Medical Diagnostics

(2017) Qingyuan Gui et al.   SENSORS

A DNA Bubble-Mediated Gene Regulation System Based on Thrombin-Bound DNA Aptamers

(2017) Jing Wang et al.   ACS Synthetic Biology

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies

(2017) Rey W. Martin et al.   ACS Synthetic Biology

A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples

(2017) Ke Yan Wen et al.   ACS Synthetic Biology

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Optical biosensors

(2016) P. Damborsky et al.   Essays in Biochemistry

Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets

(2016) M. Schwarz-Schilling et al.   Integrative Biology

Cell-Free Synthetic Biology: Engineering Beyond the Cell

(2016) Jessica G. Perez et al.   Cold Spring Harbor Perspectives in Biology

Selection and Biosensor Application of Aptamers for Small Molecules

(2016) Franziska Pfeiffer et al.   Frontiers in Chemistry

LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage

(2016) Mark Thomas Smith et al.   BIOTECHNIQUES

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

(2015) Thomas S. Bronder et al.   ACS Applied Materials & Interfaces

Biosensor Technology for Pesticides—A review

(2015) Neelam Verma et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Transcription factor-based biosensors in biotechnology: current state and future prospects

(2015) Regina Mahr et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering thermal properties of elastin-like polypeptides by incorporation of unnatural amino acids in a cell-free protein synthesis system

(2015) Christy Catherine et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Global Cancer Incidence and Mortality Rates and Trends--An Update

(2015) L. A. Torre et al.   CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION

Reprogramming of glucose, fatty acid and amino acid metabolism for cancer progression

(2015) Zhaoyong Li et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities

(2015) Baojun Wang et al.   NUCLEIC ACIDS RESEARCH

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

(2015) Emmanuel L. C. de los Santos et al.   ACS Synthetic Biology

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Transcription factor-based biosensors enlightened by the analyte

(2015) Raul Fernandez-López et al.   Frontiers in Microbiology

Cystic fibrosis

(2015) Felix Ratjen et al.   Nature Reviews Disease Primers

Aptamer-based biosensors for biomedical diagnostics

(2014) Wenhu Zhou et al.   ANALYST

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Advances in biosensors: Principle, architecture and applications

(2014) Veeradasan Perumal et al.   Journal of Applied Biomedicine

Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks

(2014) Baojun Wang et al.   NUCLEIC ACIDS RESEARCH

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

(2013) James Chappell et al.   NUCLEIC ACIDS RESEARCH

Amplifying Genetic Logic Gates

(2013) J. Bonnet et al.   SCIENCE

Thrombin-Mediated Transcriptional Regulation Using DNA Aptamers in DNA-Based Cell-Free Protein Synthesis

(2013) Sukanya Iyer et al.   ACS Synthetic Biology

Emerging Nucleic Acid–Based Tests for Point-of-Care Detection of Malaria

(2012) Michael S. Cordray et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

A modular cell-based biosensor using engineered genetic logic circuits to detect and integrate multiple environmental signals

(2012) Baojun Wang et al.   BIOSENSORS & BIOELECTRONICS

Cell-free protein synthesis: the state of the art

(2012) James W. Whittaker   BIOTECHNOLOGY LETTERS

Four steps to avoid a synthetic-biology disaster

(2012) Genya V. Dana et al.   NATURE

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Synthetic biology: applications come of age

(2010) Ahmad S. Khalil et al.   NATURE REVIEWS GENETICS

Cell-Free Escherichia coli-Based System To Screen for Quorum-Sensing Molecules Interacting with Quorum Receptor Proteins of Streptomyces coelicolor

(2009) Y.-H. Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Birth defects in the sons and daughters of women who were exposed in utero to diethylstilbestrol (DES)

(2009) L. Titus-Ernstoff et al.   INTERNATIONAL JOURNAL OF ANDROLOGY

Silica-coated alginate beads for in vitro protein synthesis via transcription/translation machinery encapsulation

(2009) Seong Yoon Lim et al.   JOURNAL OF BIOTECHNOLOGY

High-yield cell-free protein production from P-gel

(2009) Nokyoung Park et al.   Nature Protocols

Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay

(2008) T. Kawaguchi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Optical Biosensors

(2008) Sergey M. Borisov et al.   CHEMICAL REVIEWS

Sloppiness, robustness, and evolvability in systems biology

(2008) Bryan C Daniels et al.   CURRENT OPINION IN BIOTECHNOLOGY

Aptamer-based biosensors

(2007) Shiping Song et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY