CRISPR-based diagnostics

CRISPR-based DNA and RNA detection with liquid-liquid phase separation

(2021) Willem Kasper Spoelstra et al.   BIOPHYSICAL JOURNAL

CRISPR Enzyme Kinetics for Molecular Diagnostics

(2021) Ashwin Ramachandran et al.   ANALYTICAL CHEMISTRY

Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9

(2021) Chunlei Jiao et al.   SCIENCE

A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

(2021) Clark H. Cunningham et al.   EBioMedicine

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

(2020) Xusheng Wang et al.   ACS Nano

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

(2020) Chaoqun Yuan et al.   ANALYTICAL CHEMISTRY

Targeted nanopore sequencing with Cas9-guided adapter ligation

(2020) Timothy Gilpatrick et al.   NATURE BIOTECHNOLOGY

Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

(2020) Jinjin Shen et al.   Nature Communications

New CRISPR-Derived microRNA Sensing Mechanism Based on Cas12a Self-Powered and Rolling Circle Transcription-Unleashed Real-Time crRNA Recruiting

(2020) Gaoting Wang et al.   ANALYTICAL CHEMISTRY

Bioluminescent Protein–Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System

(2020) Angeliki Moutsiopoulou et al.   ANALYTICAL CHEMISTRY

Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics

(2020) Menglu Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

Virological assessment of hospitalized patients with COVID-2019

(2020) Roman Wölfel et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection

(2020) Lei Peng et al.   SENSORS AND ACTUATORS B-CHEMICAL

Testing for COVID-19

(2020) Talha Khan Burki   Lancet Respiratory Medicine

CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

(2020) Ting Zhou et al.   Advanced Science

CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage

(2020) Yijuan Bao et al.   ACS Sensors

A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection

(2020) Michael M. Kaminski et al.   Nature Biomedical Engineering

A mountable toilet system for personalized health monitoring via the analysis of excreta

(2020) Seung-min Park et al.   Nature Biomedical Engineering

COVID-19 diagnostics in context

(2020) Ralph Weissleder et al.   Science Translational Medicine

Creating CRISPR-responsive smart materials for diagnostics and programmable cargo release

(2020) Raphael V. Gayet et al.   Nature Protocols

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

SPRINT: a Cas13a-based platform for detection of small molecules

(2020) Roman S Iwasaki et al.   NUCLEIC ACIDS RESEARCH

Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofPlasmodiumspecies in symptomatic and asymptomatic malaria

(2020) Rose A. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

A deep learning approach to programmable RNA switches

(2020) Nicolaas M. Angenent-Mari et al.   Nature Communications

Sequence-to-function deep learning frameworks for engineered riboregulators

(2020) Jacqueline A. Valeri et al.   Nature Communications

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

(2019) Peiwu Qin et al.   ACS Sensors

Room Temperature Detection of Plasma Epstein–Barr Virus DNA with CRISPR–Cas13

(2019) Yetao Wu et al.   CLINICAL CHEMISTRY

An RNA-Guided Cas9 Nickase-Based Method for Universal Isothermal DNA Amplification

(2019) Ting Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-Fidelity and Rapid Quantification of miRNA Combining crRNA Programmability and CRISPR/Cas13a trans-Cleavage Activity

(2019) Yuanyue Shan et al.   ANALYTICAL CHEMISTRY

Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

(2019) Reza Hajian et al.   Nature Biomedical Engineering

FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

(2019) Jenai Quan et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip

(2019) Ning Shao et al.   ANALYTICAL CHEMISTRY

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Programmable CRISPR-responsive smart materials

(2019) Max A. English et al.   SCIENCE

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

(2019) Richard Bruch et al.   ADVANCED MATERIALS

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing

(2019) Pay Giesselmann et al.   NATURE BIOTECHNOLOGY

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization performance

(2019) Hui Kwon Kim et al.   Science Advances

CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection

(2019) Qian Chen et al.   ANALYTICAL CHEMISTRY

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

(2019) Ying Xiong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a

(2019) Florian Katzmeier et al.   PLoS One

Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples

(2018) Elizabeth A. Phillips et al.   ANALYTICAL CHEMISTRY

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

(2018) Mengqi Huang et al.   ANALYTICAL CHEMISTRY

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

EASL Recommendations on Treatment of Hepatitis C 2018

(2018) Jean-Michel Pawlotsky et al.   JOURNAL OF HEPATOLOGY

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

Molecular testing for BRAF mutations to inform melanoma treatment decisions: a move toward precision medicine

(2017) Liang Cheng et al.   MODERN PATHOLOGY

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Multiplex recording of cellular events over time on CRISPR biological tape

(2017) Ravi U. Sheth et al.   SCIENCE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care

(2016) Lorraine Lillis et al.   MOLECULAR AND CELLULAR PROBES

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

(2016) Yihao Zhang et al.   ACS Synthetic Biology

Is increased time to diagnosis and treatment in symptomatic cancer associated with poorer outcomes? Systematic review

(2015) R D Neal et al.   BRITISH JOURNAL OF CANCER

Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

(2015) De-Guo Wang et al.   MOLECULES

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Noninvasive in vivo monitoring of tissue-specific global gene expression in humans

(2014) W. Koh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

British HIV Association guidelines for the routine investigation and monitoring of adult HIV-1-infected individuals 2011

(2011) D Asboe et al.   HIV MEDICINE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples

(2010) A. M. Smith et al.   NUCLEIC ACIDS RESEARCH

Cost Analysis of Multiplex PCR Testing for Diagnosing Respiratory Virus Infections

(2009) J. B. Mahony et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols