SPRINT: a Cas13a-based platform for detection of small molecules

Structure-Specific Cleavage of an RNA Repeat Expansion with a Dimeric Small Molecule Is Advantageous over Sequence-Specific Recognition by an Oligonucleotide

(2020) Raphael I. Benhamou et al.   ACS Chemical Biology

Application of aptamers as molecular recognition elements in lateral flow assays

(2020) Ruth Reid et al.   ANALYTICAL BIOCHEMISTRY

High Affinity Binding of N2-Modified Guanine Derivatives Significantly Disrupts the Ligand Binding Pocket of the Guanine Riboswitch

(2020) Michal M. Matyjasik et al.   MOLECULES

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

A progesterone biosensor derived from microbial screening

(2020) Chloé Grazon et al.   Nature Communications

Applying CRISPR-Cas12a as a Signal Amplifier to Construct Biosensors for Non-DNA Targets in Ultralow Concentrations

(2020) Junjie Li et al.   ACS Sensors

A review: Recent advances in ultrasensitive and highly specific recognition aptasensors with various detection strategies

(2020) Shu-Rong Yan et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Cell-free biosensors for rapid detection of water contaminants

(2020) Jaeyoung K. Jung et al.   NATURE BIOTECHNOLOGY

Tuning the Binding Affinity of Heme-Responsive Biosensor for Precise and Dynamic Pathway Regulation

(2020) Jian Zhang et al.   iScience

Mechanisms of antibiotics inhibiting bacterial RNA polymerase

(2019) Hamed Mosaei et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Anti-PqsR compounds as next-generation antibacterial agents against Pseudomonas aeruginosa: A review

(2019) Vahid Soheili et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Face-time with TAR: Portraits of an HIV-1 RNA with diverse modes of effector recognition relevant for drug discovery

(2019) Sai Shashank Chavali et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Next-level riboswitch development—implementation of Capture-SELEX facilitates identification of a new synthetic riboswitch

(2019) Adrien Boussebayle et al.   NUCLEIC ACIDS RESEARCH

Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch

(2019) Mohammed Dwidar et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors

(2019) Peter L. Voyvodic et al.   Nature Communications

Automated Design of Diverse Stand-Alone Riboswitches

(2019) Michelle J. Wu et al.   ACS Synthetic Biology

Riboswitch distribution, structure, and function in bacteria

(2019) Nikolet Pavlova et al.   GENE

Evolution-guided engineering of small-molecule biosensors

(2019) Tim Snoek et al.   NUCLEIC ACIDS RESEARCH

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes

(2019) Leopoldo F. M. Machado et al.   Journal of Biological Engineering

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

(2019) Ying Xiong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A low-cost fluorescence reader for in vitro transcription and nucleic acid detection with Cas13a

(2019) Florian Katzmeier et al.   PLoS One

Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor

(2019) Walter Thavarajah et al.   ACS Synthetic Biology

Purine analogs targeting the guanine riboswitch as potential antibiotics against Clostridioides difficile

(2018) Lok-Hang Yan et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Transcriptional regulators: valuable targets for novel antibacterial strategies

(2018) Andrés González et al.   Future Medicinal Chemistry

Principles for targeting RNA with drug-like small molecules

(2018) Katherine Deigan Warner et al.   NATURE REVIEWS DRUG DISCOVERY

Challenges of ligand identification for the second wave of orphan riboswitch candidates

(2018) Etienne B. Greenlee et al.   RNA Biology

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Riboswitches and Translation Control

(2018) Ronald R. Breaker   Cold Spring Harbor Perspectives in Biology

A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

(2018) Erik K. R. Hanko et al.   ACS Synthetic Biology

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Global Epidemiology of Tuberculosis

(2018) Katherine Floyd et al.   SEMINARS IN RESPIRATORY AND CRITICAL CARE MEDICINE

Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators

(2018) Javier F. Juárez et al.   Nature Communications

Drugging the RNA World

(2018) Matthew D. Disney et al.   Cold Spring Harbor Perspectives in Biology

The Ecstasy and Agony of Assay Interference Compounds

(2017) Courtney Aldrich et al.   Journal of Chemical Information and Modeling

Recurrent RNA motifs as scaffolds for genetically encodable small-molecule biosensors

(2017) Ely B Porter et al.   Nature Chemical Biology

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Design of Artificial Riboswitches as Biosensors

(2017) Sven Findeiß et al.   SENSORS

Aptamer-based point-of-care diagnostic platforms

(2017) Abhijeet Dhiman et al.   SENSORS AND ACTUATORS B-CHEMICAL

A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria

(2017) Hung-Ju Chang et al.   ACS Synthetic Biology

A Fluorescent Split Aptamer for Visualizing RNA–RNA Assembly In Vivo

(2017) Khalid K. Alam et al.   ACS Synthetic Biology

Design and application of a lactulose biosensor

(2017) Jieyuan Wu et al.   Scientific Reports

Aptamers in analytics

(2016) Muslum Ilgu et al.   ANALYST

Nucleic Acid Biosensors: Recent Advances and Perspectives

(2016) Yan Du et al.   ANALYTICAL CHEMISTRY

Riboswitches: From living biosensors to novel targets of antibiotics

(2016) Elnaz Mehdizadeh Aghdam et al.   GENE

Tailor-made transcriptional biosensors for optimizing microbial cell factories

(2016) Brecht De Paepe et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

An in vitro selection for small molecule induced switching RNA molecules

(2016) Laura Martini et al.   METHODS

Bacterial Transcription as a Target for Antibacterial Drug Development

(2016) Cong Ma et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Non-coding RNAs as drug targets

(2016) Masayuki Matsui et al.   NATURE REVIEWS DRUG DISCOVERY

Atomic resolution mechanistic studies of ribocil: A highly selective unnatural ligand mimic of theE. coliFMN riboswitch

(2016) John A. Howe et al.   RNA Biology

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

(Dis)similar Analogues of Riboswitch Metabolites as Antibacterial Lead Compounds

(2015) Daniel Matzner et al.   JOURNAL OF MEDICINAL CHEMISTRY

A Highly Coupled Network of Tertiary Interactions in the SAM-I Riboswitch and Their Role in Regulatory Tuning

(2015) Christopher Wostenberg et al.   JOURNAL OF MOLECULAR BIOLOGY

Identification of inhibitors of bacterial RNA polymerase

(2015) Xiao Yang et al.   METHODS

Selective small-molecule inhibition of an RNA structural element

(2015) John A. Howe et al.   NATURE

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Transcription factor-based biosensors enlightened by the analyte

(2015) Raul Fernandez-López et al.   Frontiers in Microbiology

Establishment and Validation of a Non-Radioactive Method for In Vitro Transcription Assay Using Primer Extension and Quantitative Real Time PCR

(2015) Juan Wang et al.   PLoS One

A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity

(2014) Cristina Voss et al.   BMC MOLECULAR BIOLOGY

Engineering allostery

(2014) Srivatsan Raman et al.   TRENDS IN GENETICS

Older and new purine nucleoside analogs for patients with acute leukemias

(2013) Pawel Robak et al.   CANCER TREATMENT REVIEWS

The promise of riboswitches as potential antibacterial drug targets

(2013) Christina E. Lünse et al.   INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY

Engineering modular ‘ON’ RNA switches using biological components

(2013) Pablo Ceres et al.   NUCLEIC ACIDS RESEARCH

Modularity of Select Riboswitch Expression Platforms Enables Facile Engineering of Novel Genetic Regulatory Devices

(2013) Pablo Ceres et al.   ACS Synthetic Biology

Development of a 2,4-Dinitrotoluene-Responsive Synthetic Riboswitch in E. coli Cells

(2012) Molly E. Davidson et al.   ACS Chemical Biology

Cations in charge: magnesium ions in RNA folding and catalysis

(2012) Jessica C Bowman et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

De novo design of a synthetic riboswitch that regulates transcription termination

(2012) M. Wachsmuth et al.   NUCLEIC ACIDS RESEARCH

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

(2011) Jiayuan Quan et al.   Nature Protocols

Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride

(2011) J. L. Baker et al.   SCIENCE

Applications of Aptamers as Sensors

(2010) Eun Jeong Cho et al.   Annual Review of Analytical Chemistry

Roseoflavin is a natural antibacterial compound that binds to FMN riboswitches and regulates gene expression

(2010) Elaine R. Lee et al.   RNA Biology

Riboswitches: Structures and Mechanisms

(2010) A. D. Garst et al.   Cold Spring Harbor Perspectives in Biology

Riboswitches and the RNA World

(2010) R. R. Breaker   Cold Spring Harbor Perspectives in Biology

Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine-Related Metabolic Pathways

(2010) Jérôme Mulhbacher et al.   PLoS Pathogens

Adaptive Ligand Binding by the Purine Riboswitch in the Recognition of Guanine and Adenine Analogs

(2009) Sunny D. Gilbert et al.   STRUCTURE

In vitro approaches to analysis of transcription termination

(2008) Irina Artsimovitch et al.   METHODS