Cryo-EM of the ATP11C flippase reconstituted in Nanodiscs shows a distended phospholipid bilayer inner membrane around transmembrane helix 2

Visualization of the mechanosensitive ion channel MscS under membrane tension

(2021) Yixiao Zhang et al.   NATURE

Structure and transport mechanism of P5B-ATPases

(2021) Ping Li et al.   Nature Communications

Cryo-EM reveals mechanistic insights into lipid-facilitated polyamine export by human ATP13A2

(2021) Atsuhiro Tomita et al.   MOLECULAR CELL

Structures of a P4-ATPase lipid flippase in lipid bilayers

(2020) Yilin He et al.   Protein & Cell

Crystal structure of a human plasma membrane phospholipid flippase

(2020) Hanayo Nakanishi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transport Cycle of Plasma Membrane Flippase ATP11C by Cryo-EM

(2020) Hanayo Nakanishi et al.   Cell Reports

Nanodiscs: A toolkit for membrane protein science

(2020) Stephen G. Sligar et al.   PROTEIN SCIENCE

Transport mechanism of P4 ATPase phosphatidylcholine flippases

(2020) Lin Bai et al.   eLife

Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase

(2019) Maria E Falzone et al.   eLife

Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM

(2019) Valeria Kalienkova et al.   eLife

Structure and autoregulation of a P4-ATPase lipid flippase

(2019) Milena Timcenko et al.   NATURE

Cryo-EM structures capture the transport cycle of the P4-ATPase flippase

(2019) Masahiro Hiraizumi et al.   SCIENCE

A single K+-binding site in the crystal structure of the gastric proton pump

(2019) Kenta Yamamoto et al.   eLife

Distribution, dynamics and functional roles of phosphatidylserine within the cell

(2019) Jason G. Kay et al.   Cell Communication and Signaling

Autoinhibition and activation mechanisms of the eukaryotic lipid flippase Drs2p-Cdc50p

(2019) Lin Bai et al.   Nature Communications

Structure of the human lipid exporter ABCB4 in a lipid environment

(2019) Jeppe A. Olsen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phospholipid subcellular localization and dynamics

(2018) Yanbo Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal structures of the gastric proton pump

(2018) Kazuhiro Abe et al.   NATURE

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

(2018) Jasenko Zivanov et al.   IUCrJ

Nanodisc-based kinetic assays reveal distinct effects of phospholipid headgroups on the phosphoenzyme transition of sarcoplasmic reticulum Ca2+-ATPase

(2017) Kazuo Yamasaki et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Protein–phospholipid interplay revealed with crystals of a calcium pump

(2017) Yoshiyuki Norimatsu et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles: Brominated Phospholipids as Tools to Follow Its Kinetics

(2017) Cédric Montigny et al.   PLoS One

Getting to the Outer Leaflet: Physiology of Phosphatidylserine Exposure at the Plasma Membrane

(2016) Edouard M. Bevers et al.   PHYSIOLOGICAL REVIEWS

Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure

(2016) Jun Suzuki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping

(2016) Thomas Günther Pomorski et al.   PROGRESS IN LIPID RESEARCH

P4-ATPases as Phospholipid Flippases—Structure, Function, and Enigmas

(2016) Jens P. Andersen et al.   Frontiers in Physiology

Interaction Analysis of a Two-Component System Using Nanodiscs

(2016) Patrick Hörnschemeyer et al.   PLoS One

Rapid transfer of overexpressed integral membrane protein from the host membrane into soluble lipid nanodiscs without previous purification

(2015) Nazhat Shirzad-Wasei et al.   BIOLOGICAL CHEMISTRY

Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins

(2015) Y. Katoh et al.   JOURNAL OF CELL SCIENCE

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

An Apoptotic ‘Eat Me’ Signal: Phosphatidylserine Exposure

(2015) Katsumori Segawa et al.   TRENDS IN CELL BIOLOGY

A review on phospholipids and their main applications in drug delivery systems

(2015) Jing Li et al.   Asian Journal of Pharmaceutical Sciences

X-ray structure of a calcium-activated TMEM16 lipid scramblase

(2014) Janine D. Brunner et al.   NATURE

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure

(2014) K. Segawa et al.   SCIENCE

Role of Flippases, Scramblases and Transfer Proteins in Phosphatidylserine Subcellular Distribution

(2014) Hannah M. Hankins et al.   TRAFFIC

Phospholipid flippases: Building asymmetric membranes and transport vesicles

(2012) Tessy T. Sebastian et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Critical role of a transmembrane lysine in aminophospholipid transport by mammalian photoreceptor P4-ATPase ATP8A2

(2012) J. A. Coleman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The Distribution and Function of Phosphatidylserine in Cellular Membranes

(2010) Peter A. Leventis et al.   Annual Review of Biophysics

Calcium-dependent phospholipid scrambling by TMEM16F

(2010) Jun Suzuki et al.   NATURE

Structural and thermodynamic analysis of the GFP:GFP-nanobody complex

(2010) Marta H. Kubala et al.   PROTEIN SCIENCE

Structural and functional characterization of H+,K+-ATPase with bound fluorinated phosphate analogs

(2009) Kazuhiro Abe et al.   JOURNAL OF STRUCTURAL BIOLOGY

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION