Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-24148-y
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Funding
- Novo Nordisk Foundation [NNF18SA0034956]
- Lundbeck Foundation
- Foundation: Lundbeck
- Foundation: Knut and Alice Wallenberg
- Foundation: Carlsberg
- Foundation: Novo-Nordisk
- Foundation: Brodrene Hartmann
- Foundation: Agnes og Poul Friis
- Foundation: Augustinus
- Foundation: Crafoord
- Foundation: The Per-Eric and Ulla Schyberg
- Independent Research Fund Denmark
- Swedish Research Council
- Michaelsen scholarship
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In this study, cryo-EM structures of a yeast homolog of human ATP13A2-5, Ypk9, were used to depict three separate transport cycle intermediates, including spermine-bound conformations. The findings suggest a shared principle of transport and regulation among evolutionary related P4-, P5A- and P5B-ATPases, providing insights for the analysis of associated disorders such as Parkinson's disease.
In human cells, P5B-ATPases execute export of spermine from lysosomes to the cytosol, but the mechanisms of spermine recognition, uptake and transport remain elusive. Here the authors present cryo-EM structures of a yeast homolog of human ATP13A2-5, Ypk9, which depict three separate transport cycle intermediates, including spermine-bound conformations In human cells, P5B-ATPases execute the active export of physiologically important polyamines such as spermine from lysosomes to the cytosol, a function linked to a palette of disorders. Yet, the overall shape of P5B-ATPases and the mechanisms of polyamine recognition, uptake and transport remain elusive. Here we describe a series of cryo-electron microscopy structures of a yeast homolog of human ATP13A2-5, Ypk9, determined at resolutions reaching 3.4 angstrom, and depicting three separate transport cycle intermediates, including spermine-bound conformations. Surprisingly, in the absence of cargo, Ypk9 rests in a phosphorylated conformation auto-inhibited by the N-terminus. Spermine uptake is accomplished through an electronegative cleft lined by transmembrane segments 2, 4 and 6. Despite the dramatically different nature of the transported cargo, these findings pinpoint shared principles of transport and regulation among the evolutionary related P4-, P5A- and P5B-ATPases. The data also provide a framework for analysis of associated maladies, such as Parkinson's disease.
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