Cryo-EM structures capture the transport cycle of the P4-ATPase flippase

Asparagine-905 of the mammalian phospholipid flippase ATP8A2 is essential for lipid substrate-induced activation of ATP8A2 dephosphorylation

(2019) Stine A. Mikkelsen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolution and a revised nomenclature of P4 ATPases, a eukaryotic family of lipid flippases

(2019) Michael Palmgren et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Phospholipid subcellular localization and dynamics

(2018) Yanbo Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal structures of the gastric proton pump

(2018) Kazuhiro Abe et al.   NATURE

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

New tools for the analysis and validation of cryo-EM maps and atomic models

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

Substrates of P4-ATPases: beyond aminophospholipids (phosphatidylserine and phosphatidylethanolamine)

(2018) Hye-Won Shin et al.   FASEB JOURNAL

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

(2018) Jasenko Zivanov et al.   IUCrJ

The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane

(2017) Katsumori Segawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity

(2017) Madhavan Chalat et al.   MOLECULAR BIOLOGY OF THE CELL

Protein–phospholipid interplay revealed with crystals of a calcium pump

(2017) Yoshiyuki Norimatsu et al.   NATURE

Dynamics of P-type ATPase transport revealed by single-molecule FRET

(2017) Mateusz Dyla et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Disease mutations reveal residues critical to the interaction of P4-ATPases with lipid substrates

(2017) Rasmus H. Gantzel et al.   Scientific Reports

On the molecular mechanism of flippase- and scramblase-mediated phospholipid transport

(2016) Cédric Montigny et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase

(2016) Bartholomew P. Roland et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta

(2016) Ray Yu-Ruei Wang et al.   eLife

P4-ATPases as Phospholipid Flippases—Structure, Function, and Enigmas

(2016) Jens P. Andersen et al.   Frontiers in Physiology

Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase

(2015) S. Lee et al.   EMBO JOURNAL

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex

(2015) Mette Laursen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

(2015) Alan Brown et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Critical roles of isoleucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2

(2014) A. L. Vestergaard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure

(2014) K. Segawa et al.   SCIENCE

Role of Flippases, Scramblases and Transfer Proteins in Phosphatidylserine Subcellular Distribution

(2014) Hannah M. Hankins et al.   TRAFFIC

P4 ATPases: Flippases in Health and Disease

(2013) Vincent van der Mark et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Auto-inhibition of Drs2p, a Yeast Phospholipid Flippase, by Its Carboxyl-terminal Tail

(2013) Xiaoming Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal structures of the calcium pump and sarcolipin in the Mg2+-bound E1 state

(2013) Chikashi Toyoshima et al.   NATURE

Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases

(2013) R. D. Baldridge et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Missense mutation in the ATPase, aminophospholipid transporter protein ATP8A2 is associated with cerebellar atrophy and quadrupedal locomotion

(2012) Onur Emre Onat et al.   EUROPEAN JOURNAL OF HUMAN GENETICS

Role for Phospholipid Flippase Complex of ATP8A1 and CDC50A Proteins in Cell Migration

(2012) Utako Kato et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphatidylserine Stimulation of Drs2p·Cdc50p Lipid Translocase Dephosphorylation Is Controlled by Phosphatidylinositol-4-phosphate

(2012) Aurore Jacquot et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Critical role of a transmembrane lysine in aminophospholipid transport by mammalian photoreceptor P4-ATPase ATP8A2

(2012) J. A. Coleman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases

(2012) R. D. Baldridge et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Critical Role of the β-Subunit CDC50A in the Stable Expression, Assembly, Subcellular Localization, and Lipid Transport Activity of the P4-ATPase ATP8A2

(2011) Jonathan A. Coleman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Atp8a1 deficiency is associated with phosphatidylserine externalization in hippocampus and delayed hippocampus-dependent learning

(2011) Kelly Levano et al.   JOURNAL OF NEUROCHEMISTRY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

CDC50 Proteins Are Critical Components of the Human Class-1 P4-ATPase Transport Machinery

(2010) Susanne Bryde et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Localization, Purification, and Functional Reconstitution of the P4-ATPase Atp8a2, a Phosphatidylserine Flippase in Photoreceptor Disc Membranes

(2009) Jonathan A. Coleman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of a Golgi flippase by phosphoinositides and an ArfGEF

(2009) Paramasivam Natarajan et al.   NATURE CELL BIOLOGY

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY