Clustering of Aromatic Residues in Prion-like Domains Can Tune the Formation, State, and Organization of Biomolecular Condensates

Designer Condensates: A Toolkit for the Biomolecular Architect

(2021) Renee L. Hastings et al.   JOURNAL OF MOLECULAR BIOLOGY

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

(2021) Georg Krainer et al.   Nature Communications

Metapredict: a fast, accurate, and easy-to-use predictor of consensus disorder and structure

(2021) Ryan J. Emenecker et al.   BIOPHYSICAL JOURNAL

A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation

(2021) Yanniv Dorone et al.   CELL

Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis

(2021) Ryan J. Emenecker et al.   Cell Communication and Signaling

Phase separation vs aggregation behavior for model disordered proteins

(2021) Ushnish Rana et al.   JOURNAL OF CHEMICAL PHYSICS

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Analyses on clustering of the conserved residues at protein-RNA interfaces and its application in binding site identification

(2020) Zhen Yang et al.   BMC BIOINFORMATICS

Model for disordered proteins with strongly sequence-dependent liquid phase behavior

(2020) Antonia Statt et al.   JOURNAL OF CHEMICAL PHYSICS

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization

(2020) David W. Sanders et al.   CELL

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior

(2020) Benjamin S. Schuster et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic metastable long-living droplets formed by sticker-spacer proteins

(2020) Srivastav Ranganathan et al.   eLife

Connecting Coil-to-Globule Transitions to Full Phase Diagrams for Intrinsically Disordered Proteins

(2020) Xiangze Zeng et al.   BIOPHYSICAL JOURNAL

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

Tunable multiphase dynamics of arginine and lysine liquid condensates

(2020) Rachel S. Fisher et al.   Nature Communications

A Prion-like Domain in Transcription Factor EBF1 Promotes Phase Separation and Enables B Cell Programming of Progenitor Chromatin

(2020) Yuanting Wang et al.   IMMUNITY

Revealing the Hidden Sensitivity of Intrinsically Disordered Proteins to their Chemical Environment

(2020) David Moses et al.   Journal of Physical Chemistry Letters

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Complete Phase Diagram for Liquid–Liquid Phase Separation of Intrinsically Disordered Proteins

(2019) James McCarty et al.   Journal of Physical Chemistry Letters

Cellular sensing by phase separation: Using the process, not just the products

(2019) Haneul Yoo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single Amino Acid Substitutions in Stickers, but Not Spacers, Substantially Alter UBQLN2 Phase Transitions and Dense Phase Material Properties

(2019) Yiran Yang et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

(2019) Giorgia Celetti et al.   JOURNAL OF CELL BIOLOGY

Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana

(2019) Samantha K. Powers et al.   MOLECULAR CELL

The Control Centers of Biomolecular Phase Separation: How Membrane Surfaces, PTMs, and Active Processes Regulate Condensation

(2019) Wilton T. Snead et al.   MOLECULAR CELL

Loss of Dynamic RNA Interaction and Aberrant Phase Separation Induced by Two Distinct Types of ALS/FTD-Linked FUS Mutations

(2019) Amirhossein Ghanbari Niaki et al.   MOLECULAR CELL

PhaSepDB: a database of liquid–liquid phase separation related proteins

(2019) Kaiqiang You et al.   NUCLEIC ACIDS RESEARCH

Effective concentrations enforced by intrinsically disordered linkers are governed by polymer physics

(2019) Charlotte S. Sørensen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The mutational landscape of a prion-like domain

(2019) Benedetta Bolognesi et al.   Nature Communications

Phase separation-deficient TDP43 remains functional in splicing

(2019) Hermann Broder Schmidt et al.   Nature Communications

LASSI: A lattice model for simulating phase transitions of multivalent proteins

(2019) Jeong-Mo Choi et al.   PLoS Computational Biology

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites

(2018) Takuya Yoshizawa et al.   CELL

Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation

(2018) Mario Hofweber et al.   CELL

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

A Lattice Model of Charge-Pattern-Dependent Polyampholyte Phase Separation

(2018) Suman Das et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks

(2018) Michael P. Hughes et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Organization and Function of Non-dynamic Biomolecular Condensates

(2018) Jeffrey B. Woodruff et al.   TRENDS IN BIOCHEMICAL SCIENCES

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Pi-Pi contacts are an overlooked protein feature relevant to phase separation

(2018) Robert McCoy Vernon et al.   eLife

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

(2018) Benjamin S. Schuster et al.   Nature Communications

Intrinsically disordered sequences enable modulation of protein phase separation through distributed tyrosine motifs

(2017) Yuan Lin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics

(2017) Steven Boeynaems et al.   MOLECULAR CELL

Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation

(2017) Thomas C. Boothby et al.   MOLECULAR CELL

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins

(2017) Tyler S Harmon et al.   eLife

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

The balbiani body and the concept of physiological amyloids

(2016) Elvan Boke et al.   CELL CYCLE

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles

(2016) Yi-Hsuan Lin et al.   PHYSICAL REVIEW LETTERS

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

Transport Selectivity of Nuclear Pores, Phase Separation, and Membraneless Organelles

(2016) H. Broder Schmidt et al.   TRENDS IN BIOCHEMICAL SCIENCES

In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes

(2016) Hermann Broder Schmidt et al.   Cell Reports

A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

(2016) Matthias Christoph Munder et al.   eLife

Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors

(2015) Sigrid Milles et al.   CELL

The LC Domain of hnRNPA2 Adopts Similar Conformations in Hydrogel Polymers, Liquid-like Droplets, and Nuclei

(2015) Siheng Xiang et al.   CELL

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

(2015) Felipe García Quiroz et al.   NATURE MATERIALS

ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function

(2015) Tetsuro Murakami et al.   NEURON

RNA transcription modulates phase transition-driven nuclear body assembly

(2015) Joel Berry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulated assembly of a supramolecular centrosome scaffold in vitro

(2015) J. B. Woodruff et al.   SCIENCE

Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity

(2015) Hermann Broder Schmidt et al.   eLife

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

PLAAC: a web and command-line application to identify proteins with prion-like amino acid composition

(2014) A. K. Lancaster et al.   BIOINFORMATICS

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

(2014) Jennifer T Wang et al.   eLife

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

(2013) Arnaud Hubstenberger et al.   DEVELOPMENTAL CELL

Mutations in prion-like domains in hnRNPA2B1 and hnRNPA1 cause multisystem proteinopathy and ALS

(2013) Hong Joo Kim et al.   NATURE

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Getting RNA and Protein in Phase

(2012) Stephanie C. Weber et al.   CELL

Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies

(2012) Tina W. Han et al.   CELL

Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes

(2012) Aksana A Labokha et al.   EMBO JOURNAL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Desiccation-Induced Structuralization and Glass Formation of Group 3 Late Embryogenesis Abundant Protein Model Peptides

(2009) Tempei Shimizu et al.   BIOCHEMISTRY

A Systematic Survey Identifies Prions and Illuminates Sequence Features of Prionogenic Proteins

(2009) Simon Alberti et al.   CELL

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE