Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis

The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

(2021) Jasmine Cubuk et al.   Nature Communications

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Controlling Structural Bias in Intrinsically Disordered Proteins Using Solution Space Scanning

(2020) Alex S. Holehouse et al.   Journal of Chemical Theory and Computation

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior

(2020) Benjamin S. Schuster et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Making the Case for Disordered Proteins and Biomolecular Condensates in Bacteria

(2020) Megan C. Cohan et al.   TRENDS IN BIOCHEMICAL SCIENCES

Emerging Roles for Phase Separation in Plants

(2020) Ryan J. Emenecker et al.   DEVELOPMENTAL CELL

Revealing the Hidden Sensitivity of Intrinsically Disordered Proteins to their Chemical Environment

(2020) David Moses et al.   Journal of Physical Chemistry Letters

Protein condensates as aging Maxwell fluids

(2020) Louise Jawerth et al.   SCIENCE

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ALS-Linked Mutations Affect UBQLN2 Oligomerization and Phase Separation in a Position- and Amino Acid-Dependent Manner

(2019) Thuy P. Dao et al.   STRUCTURE

Cryo-EM structures of four polymorphic TDP-43 amyloid cores

(2019) Qin Cao et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana

(2019) Samantha K. Powers et al.   MOLECULAR CELL

The mutational landscape of a prion-like domain

(2019) Benedetta Bolognesi et al.   Nature Communications

Phase separation-deficient TDP43 remains functional in splicing

(2019) Hermann Broder Schmidt et al.   Nature Communications

Regulation of auxin transcriptional responses

(2019) Samantha K. Powers et al.   DEVELOPMENTAL DYNAMICS

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing

(2018) Ailin Wang et al.   EMBO JOURNAL

Prion-like low-complexity sequences: Key regulators of protein solubility and phase behavior

(2018) Titus Franzmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Profilin reduces aggregation and phase separation of huntingtin N-terminal fragments by preferentially binding to soluble monomers and oligomers

(2018) Ammon E. Posey et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Liquid to Solid Phase Transition Underlying Pathological Huntingtin Exon1 Aggregation

(2018) Thomas R. Peskett et al.   MOLECULAR CELL

Atomic structures of TDP-43 LCD segments and insights into reversible or pathogenic aggregation

(2018) Elizabeth L. Guenther et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Organization and Function of Non-dynamic Biomolecular Condensates

(2018) Jeffrey B. Woodruff et al.   TRENDS IN BIOCHEMICAL SCIENCES

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Atomic structures of TDP-43 LCD segments and insights into reversible or pathogenic aggregation

(2018) Elizabeth L. Guenther et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Origin and evolution of the nuclear auxin response system

(2018) Sumanth K Mutte et al.   eLife

Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments

(2018) Jill J. Bouchard et al.   MOLECULAR CELL

Intramolecular Interactions Overcome Hydration to Drive the Collapse Transition of Gly15

(2017) D. Asthagiri et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Phase separation drives heterochromatin domain formation

(2017) Amy R. Strom et al.   NATURE

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin

(2017) Adam G. Larson et al.   NATURE

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Affinity of IDPs to their targets is modulated by ion-specific changes in kinetics and residual structure

(2017) Basile I. M. Wicky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Amyloid-like Self-Assembly of a Cellular Compartment

(2016) Elvan Boke et al.   CELL

Adaptation to Stressors by Systemic Protein Amyloidogenesis

(2016) Timothy E. Audas et al.   DEVELOPMENTAL CELL

Higher‐order oligomerization promotes localization of SPOP to liquid nuclear speckles

(2016) Melissa R Marzahn et al.   EMBO JOURNAL

Insights into Coupled Folding and Binding Mechanisms from Kinetic Studies

(2016) Sarah L. Shammas et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

Dynamic microfluidic control of supramolecular peptide self-assembly

(2016) Zohar A. Arnon et al.   Nature Communications

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Relating sequence encoded information to form and function of intrinsically disordered proteins

(2015) Rahul K Das et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Quantitative Assessments of the Distinct Contributions of Polypeptide Backbone Amides versus Side Chain Groups to Chain Expansion via Chemical Denaturation

(2015) Alex S. Holehouse et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein collapse driven against solvation free energy without H-bonds

(2015) Deepti Karandur et al.   PROTEIN SCIENCE

Regulated assembly of a supramolecular centrosome scaffold in vitro

(2015) J. B. Woodruff et al.   SCIENCE

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

PLAAC: a web and command-line application to identify proteins with prion-like amino acid composition

(2014) A. K. Lancaster et al.   BIOINFORMATICS

Classification of Intrinsically Disordered Regions and Proteins

(2014) Robin van der Lee et al.   CHEMICAL REVIEWS

Coupled Folding and Binding of the Disordered Protein PUMA Does Not Require Particular Residual Structure

(2014) Joseph M. Rogers et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression

(2014) D. A. Korasick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Translation Repressors, an RNA Helicase, and Developmental Cues Control RNP Phase Transitions during Early Development

(2013) Arnaud Hubstenberger et al.   DEVELOPMENTAL CELL

Unmasking the roles of N- and C-terminal flanking sequences from exon 1 of huntingtin as modulators of polyglutamine aggregation

(2013) S. L. Crick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies

(2012) Tina W. Han et al.   CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

The complexity and implications of yeast prion domains

(2012) Zhiqiang Du   Prion

Backbone-Driven Collapse in Unfolded Protein Chains

(2011) Daniel P. Teufel et al.   JOURNAL OF MOLECULAR BIOLOGY

Essential Role of Coiled Coils for Aggregation and Activity of Q/N-Rich Prions and PolyQ Proteins

(2010) Ferdinando Fiumara et al.   CELL

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

(2009) Fu-Hui Wu et al.   Plant Methods

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

A polymer physics perspective on driving forces and mechanisms for protein aggregation

(2007) Rohit V. Pappu et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL