Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing

Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect

(2021) Yu-Fan Chuang et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Prime editing – an update on the field

(2021) Janine Scholefield et al.   GENE THERAPY

Novel Engineered Programmable Systems for ADAR-Mediated RNA Editing

(2020) Guillermo Aquino-Jarquin   Molecular Therapy-Nucleic Acids

Variability in Genome Editing Outcomes: Challenges for Research Reproducibility and Clinical Safety

(2020) Lydia Teboul et al.   MOLECULAR THERAPY

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

(2020) Nicole D. Marino et al.   NATURE METHODS

CRISPR off-target detection with DISCOVER-seq

(2020) Beeke Wienert et al.   Nature Protocols

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

Progress and challenges towards CRISPR/Cas clinical translation

(2020) Daniel Rosenblum et al.   ADVANCED DRUG DELIVERY REVIEWS

Current Status and Challenges of DNA Base Editing Tools

(2020) You Kyeong Jeong et al.   MOLECULAR THERAPY

CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity

(2020) Cicera R. Lazzarotto et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

The delivery challenge: fulfilling the promise of therapeutic genome editing

(2020) Joost van Haasteren et al.   NATURE BIOTECHNOLOGY

Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

(2020) Mu-Sen Liu et al.   Nature Communications

Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing

(2020) Muhammad Naeem et al.   Cells

Anticipating and Identifying Collateral Damage in Genome Editing

(2020) Gaëtan Burgio et al.   TRENDS IN GENETICS

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

(2020) Matthew A. Coelho et al.   Nature Communications

Various Aspects of a Gene Editing System—CRISPR–Cas9

(2020) Edyta Janik et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR-Cas “Non-Target” Sites Inhibit On-Target Cutting Rates

(2020) Eirik A. Moreb et al.   CRISPR Journal

Methodologies for Improving HDR Efficiency

(2019) Mingjie Liu et al.   Frontiers in Genetics

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

(2019) Grégoire Cullot et al.   Nature Communications

A New Class of Medicines through DNA Editing

(2019) Matthew H. Porteus   NEW ENGLAND JOURNAL OF MEDICINE

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

(2019) Yalan Zhu et al.   MOLECULAR CELL

Adenine base editing in an adult mouse model of tyrosinaemia

(2019) Chun-Qing Song et al.   Nature Biomedical Engineering

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

Optimal sequencing depth design for whole genome re-sequencing in pigs

(2019) Yifan Jiang et al.   BMC BIOINFORMATICS

Engineering guide RNA to reduce the off-target effects of CRISPR

(2019) Jing Wu et al.   Journal of Genetics and Genomics

Got mutation? ‘Base editors’ fix genomes one nucleotide at a time

(2019) Sandeep Ravindran   NATURE

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

A prime alternative

(2019) Linda Koch   NATURE REVIEWS GENETICS

Improved Nucleic Acid Therapy with Advanced Nanoscale Biotechnology

(2019) Yuhua Weng et al.   Molecular Therapy-Nucleic Acids

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

UDiTaS™, a genome editing detection method for indels and genome rearrangements

(2018) Georgia Giannoukos et al.   BMC GENOMICS

CRISPR–Cas13 Precision Transcriptome Engineering in Cancer

(2018) Javier T. Granados-Riveron et al.   CANCER RESEARCH

Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

(2018) Seuk-Min Ryu et al.   NATURE BIOTECHNOLOGY

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

(2018) Hao Yin et al.   Nature Chemical Biology

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Initiation of homologous recombination at DNA nicks

(2018) Nancy Maizels et al.   NUCLEIC ACIDS RESEARCH

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

(2018) Christopher R. Cromwell et al.   Nature Communications

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

In Vivo Genome Editing as a Therapeutic Approach

(2018) Beatrice Ho et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

In vivo CRISPR editing with no detectable genome-wide off-target mutations

(2018) Pinar Akcakaya et al.   NATURE

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants

(2018) Jianying Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Staphylococcus aureus Cas9 is a multiple-turnover enzyme

(2018) Paul Yourik et al.   RNA

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein

(2017) De Dong et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

(2017) Shengdar Q Tsai et al.   NATURE METHODS

Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

(2017) Daniel E Ryan et al.   NUCLEIC ACIDS RESEARCH

Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome Editing

(2017) Namita Bisaria et al.   Cell Systems

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

A genome-editing strategy to treat β-hemoglobinopathies that recapitulates a mutation associated with a benign genetic condition

(2016) Elizabeth A Traxler et al.   NATURE MEDICINE

Detecting DNA double-stranded breaks in mammalian genomes by linear amplification–mediated high-throughput genome-wide translocation sequencing

(2016) Jiazhi Hu et al.   Nature Protocols

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells

(2016) M. A. DeWitt et al.   Science Translational Medicine

Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation

(2016) James I. McDonald et al.   Biology Open

CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

(2016) Ting-Wei Will Chiang et al.   Scientific Reports

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

(2016) Jian-Ping Zhang et al.   Scientific Reports

Genome Editing Technologies: Defining a Path to Clinic

(2015) Jacqueline Corrigan-Curay et al.   MOLECULAR THERAPY

Chromothripsis from DNA damage in micronuclei

(2015) Cheng-Zhong Zhang et al.   NATURE

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

(2015) Daesik Kim et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice

(2015) Katerina Kraft et al.   Cell Reports

siRNA Versus miRNA as Therapeutics for Gene Silencing

(2015) Jenny K W Lam et al.   Molecular Therapy-Nucleic Acids

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

(2014) Richard L Frock et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

(2014) Y. Lin et al.   NUCLEIC ACIDS RESEARCH

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

(2013) Thomas J. Cradick et al.   NUCLEIC ACIDS RESEARCH

Mammalian Base Excision Repair: the Forgotten Archangel

(2013) Grigory L. Dianov et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

(2012) Joe Bondy-Denomy et al.   NATURE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM